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s-Methyl cysteine enhanced survival of nerve growth factor differentiated PC12 cells under hypoxic conditions.
Food Funct. 2014 Jun; 5(6):1125-33.FF

Abstract

A nerve growth factor-differentiated PC12 cell line was used to investigate the protective effects of s-methyl cysteine (SMC) at 1, 2, 4, and 8 μM under oxygen-glucose deprivation (OGD) conditions. OGD decreased the cell viability. However, SMC pre-treatments at 2, 4 and 8 μM improved the cell viability, decreased cleaved caspase-3 and Bax expression, and reserved Bcl-2 expression. Furthermore, SMC maintained the mitochondrial membrane potential, lowered the intracellular Ca(2+) concentration and DNA fragmentation, and decreased the activity and expression of caspase-3 and caspase-8. OGD increased the reactive oxygen species (ROS) and 3-nitrotyrosine production, decreased glutathione peroxide (GPX) and glutathione reductase (GR) activities and the expression, enhanced nitric oxide synthase (NOS) activity and inducible NOS (iNOS) expression. SMC pre-treatments at 2, 4 and 8 μM lowered the ROS and 3-nitrotyrosine formation, maintained GPX and GR activities and expression, and decreased NOS activity and iNOS expression. OGD up-regulated hypoxia-inducible factor (HIF)-1α, nuclear transcription factor kappa (NF-κ) B p50, NF-κB p65 and p-p38 expression. SMC pre-treatments at 1-8 μM lowered HIF-1α expression and decreased p38 phosphorylation. SMC at 2, 4 and 8 μM suppressed the protein expression of NF-κB p50 and NF-κB p65. When YC-1 (HIF-1α inhibitor), pyrrolidine dithiocarbamate (NF-κB inhibitor) or SB203580 (p38MAPK inhibitor) were used to block the activation of HIF-1α, NF-κB and p38, SMC pre-treatments did not affect the protein expression of HIF-1α, NF-κB and p-p38. These results indicated that SMC was a potent neuro-protective agent.

Authors+Show Affiliations

Department of Neurosurgery, China Medical University Hospital, Taichung City, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24710107

Citation

Liu, Chun-Lin, et al. "S-Methyl Cysteine Enhanced Survival of Nerve Growth Factor Differentiated PC12 Cells Under Hypoxic Conditions." Food & Function, vol. 5, no. 6, 2014, pp. 1125-33.
Liu CL, Hsia TC, Yin MC. S-Methyl cysteine enhanced survival of nerve growth factor differentiated PC12 cells under hypoxic conditions. Food Funct. 2014;5(6):1125-33.
Liu, C. L., Hsia, T. C., & Yin, M. C. (2014). S-Methyl cysteine enhanced survival of nerve growth factor differentiated PC12 cells under hypoxic conditions. Food & Function, 5(6), 1125-33. https://doi.org/10.1039/c3fo60689a
Liu CL, Hsia TC, Yin MC. S-Methyl Cysteine Enhanced Survival of Nerve Growth Factor Differentiated PC12 Cells Under Hypoxic Conditions. Food Funct. 2014;5(6):1125-33. PubMed PMID: 24710107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - s-Methyl cysteine enhanced survival of nerve growth factor differentiated PC12 cells under hypoxic conditions. AU - Liu,Chun-Lin, AU - Hsia,Te-Chun, AU - Yin,Mei-Chin, Y1 - 2014/04/08/ PY - 2014/4/9/entrez PY - 2014/4/9/pubmed PY - 2015/1/30/medline SP - 1125 EP - 33 JF - Food & function JO - Food Funct VL - 5 IS - 6 N2 - A nerve growth factor-differentiated PC12 cell line was used to investigate the protective effects of s-methyl cysteine (SMC) at 1, 2, 4, and 8 μM under oxygen-glucose deprivation (OGD) conditions. OGD decreased the cell viability. However, SMC pre-treatments at 2, 4 and 8 μM improved the cell viability, decreased cleaved caspase-3 and Bax expression, and reserved Bcl-2 expression. Furthermore, SMC maintained the mitochondrial membrane potential, lowered the intracellular Ca(2+) concentration and DNA fragmentation, and decreased the activity and expression of caspase-3 and caspase-8. OGD increased the reactive oxygen species (ROS) and 3-nitrotyrosine production, decreased glutathione peroxide (GPX) and glutathione reductase (GR) activities and the expression, enhanced nitric oxide synthase (NOS) activity and inducible NOS (iNOS) expression. SMC pre-treatments at 2, 4 and 8 μM lowered the ROS and 3-nitrotyrosine formation, maintained GPX and GR activities and expression, and decreased NOS activity and iNOS expression. OGD up-regulated hypoxia-inducible factor (HIF)-1α, nuclear transcription factor kappa (NF-κ) B p50, NF-κB p65 and p-p38 expression. SMC pre-treatments at 1-8 μM lowered HIF-1α expression and decreased p38 phosphorylation. SMC at 2, 4 and 8 μM suppressed the protein expression of NF-κB p50 and NF-κB p65. When YC-1 (HIF-1α inhibitor), pyrrolidine dithiocarbamate (NF-κB inhibitor) or SB203580 (p38MAPK inhibitor) were used to block the activation of HIF-1α, NF-κB and p38, SMC pre-treatments did not affect the protein expression of HIF-1α, NF-κB and p-p38. These results indicated that SMC was a potent neuro-protective agent. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/24710107/s_Methyl_cysteine_enhanced_survival_of_nerve_growth_factor_differentiated_PC12_cells_under_hypoxic_conditions_ L2 - https://doi.org/10.1039/c3fo60689a DB - PRIME DP - Unbound Medicine ER -