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Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.
Am J Trop Med Hyg. 2014 Jun; 90(6):1043-6.AJ

Abstract

Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.

Authors+Show Affiliations

Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand amh9@cdc.gov.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24710616

Citation

Duval, Brea D., et al. "Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia Pseudomallei and Burkholderia Mallei." The American Journal of Tropical Medicine and Hygiene, vol. 90, no. 6, 2014, pp. 1043-6.
Duval BD, Elrod MG, Gee JE, et al. Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei. Am J Trop Med Hyg. 2014;90(6):1043-6.
Duval, B. D., Elrod, M. G., Gee, J. E., Chantratita, N., Tandhavanant, S., Limmathurotsakul, D., & Hoffmaster, A. R. (2014). Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei. The American Journal of Tropical Medicine and Hygiene, 90(6), 1043-6. https://doi.org/10.4269/ajtmh.14-0025
Duval BD, et al. Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia Pseudomallei and Burkholderia Mallei. Am J Trop Med Hyg. 2014;90(6):1043-6. PubMed PMID: 24710616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei. AU - Duval,Brea D, AU - Elrod,Mindy G, AU - Gee,Jay E, AU - Chantratita,Narisara, AU - Tandhavanant,Sarunporn, AU - Limmathurotsakul,Direk, AU - Hoffmaster,Alex R, Y1 - 2014/04/07/ PY - 2014/4/9/entrez PY - 2014/4/9/pubmed PY - 2014/8/27/medline SP - 1043 EP - 6 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 90 IS - 6 N2 - Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/24710616/Evaluation_of_a_latex_agglutination_assay_for_the_identification_of_Burkholderia_pseudomallei_and_Burkholderia_mallei_ L2 - http://www.ajtmh.org/content/journals/10.4269/ajtmh.14-0025?crawler=true&mimetype=application/pdf DB - PRIME DP - Unbound Medicine ER -