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Long-chain fatty acid oxidation changes in a β2 glycoprotein I-induced preeclampsia-like mouse model.
Placenta. 2014 Jun; 35(6):392-7.P

Abstract

INTRODUCTION

Abnormal fatty acid oxidation (FAO) and lipid metabolism have been found related to preeclampsia (PE). Antiphospholipid syndrome (APS) as a clinical risk factor for PE has also been reported with abnormal lipid metabolism. However, the role of FAO in PE accompanied with APS is unknown. We aimed to investigate long-chain FAO changes in a PE-like rodent model induced by beta 2-glycoprotein I (β2GPI).

METHODS

The PE-like model was established by injection of β2GPI (β2GPI group) or normal saline (control group) into C57BL/6J mice which were sacrificed on day 14 or 18 of gestation. Serum levels of anti-cardiolipin antibodies (aCL), anti-β2GPI antibodies (aβ2GPI) and serum lipids were assayed. Lipid deposition in the placenta and maternal liver was detected by lipid staining. Long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) mRNA and protein expression in the placenta and maternal liver was analyzed.

RESULTS

The β2GPI group showed PE-like symptoms including hypertension, proteinuria and adverse pregnancy outcomes. Serum aCL, aβ2GPI, free fatty acid (FFA) and triglyceride (TG) levels in the β2GPI group were significantly elevated compared with the corresponding control group (P < 0.05), while cholesterol showed no significant changes. Placenta and maternal liver fatty infiltration was found in the β2GPI group. LCHAD mRNA and protein expression in the placenta and maternal liver in the β2GPI group were significantly elevated compared with the corresponding control group (P < 0.05).

CONCLUSION

β2GPI can induce PE-like symptoms, elevated serum FFA and TG, and abnormal LCHAD expression in pregnant mice. Changes in long-chain FAO could be a factor linking PE and APS.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China.Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China. Electronic address: zi_yang@email.com.Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China.Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24713206

Citation

Ding, X, et al. "Long-chain Fatty Acid Oxidation Changes in a Β2 Glycoprotein I-induced Preeclampsia-like Mouse Model." Placenta, vol. 35, no. 6, 2014, pp. 392-7.
Ding X, Yang Z, Han Y, et al. Long-chain fatty acid oxidation changes in a β2 glycoprotein I-induced preeclampsia-like mouse model. Placenta. 2014;35(6):392-7.
Ding, X., Yang, Z., Han, Y., & Yu, H. (2014). Long-chain fatty acid oxidation changes in a β2 glycoprotein I-induced preeclampsia-like mouse model. Placenta, 35(6), 392-7. https://doi.org/10.1016/j.placenta.2014.03.013
Ding X, et al. Long-chain Fatty Acid Oxidation Changes in a Β2 Glycoprotein I-induced Preeclampsia-like Mouse Model. Placenta. 2014;35(6):392-7. PubMed PMID: 24713206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-chain fatty acid oxidation changes in a β2 glycoprotein I-induced preeclampsia-like mouse model. AU - Ding,X, AU - Yang,Z, AU - Han,Y, AU - Yu,H, Y1 - 2014/03/26/ PY - 2013/12/20/received PY - 2014/03/12/revised PY - 2014/03/18/accepted PY - 2014/4/10/entrez PY - 2014/4/10/pubmed PY - 2015/1/13/medline KW - Antiphospholipid syndrome KW - Fatty acid oxidation KW - Preeclampsia SP - 392 EP - 7 JF - Placenta JO - Placenta VL - 35 IS - 6 N2 - INTRODUCTION: Abnormal fatty acid oxidation (FAO) and lipid metabolism have been found related to preeclampsia (PE). Antiphospholipid syndrome (APS) as a clinical risk factor for PE has also been reported with abnormal lipid metabolism. However, the role of FAO in PE accompanied with APS is unknown. We aimed to investigate long-chain FAO changes in a PE-like rodent model induced by beta 2-glycoprotein I (β2GPI). METHODS: The PE-like model was established by injection of β2GPI (β2GPI group) or normal saline (control group) into C57BL/6J mice which were sacrificed on day 14 or 18 of gestation. Serum levels of anti-cardiolipin antibodies (aCL), anti-β2GPI antibodies (aβ2GPI) and serum lipids were assayed. Lipid deposition in the placenta and maternal liver was detected by lipid staining. Long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) mRNA and protein expression in the placenta and maternal liver was analyzed. RESULTS: The β2GPI group showed PE-like symptoms including hypertension, proteinuria and adverse pregnancy outcomes. Serum aCL, aβ2GPI, free fatty acid (FFA) and triglyceride (TG) levels in the β2GPI group were significantly elevated compared with the corresponding control group (P < 0.05), while cholesterol showed no significant changes. Placenta and maternal liver fatty infiltration was found in the β2GPI group. LCHAD mRNA and protein expression in the placenta and maternal liver in the β2GPI group were significantly elevated compared with the corresponding control group (P < 0.05). CONCLUSION: β2GPI can induce PE-like symptoms, elevated serum FFA and TG, and abnormal LCHAD expression in pregnant mice. Changes in long-chain FAO could be a factor linking PE and APS. SN - 1532-3102 UR - https://www.unboundmedicine.com/medline/citation/24713206/Long_chain_fatty_acid_oxidation_changes_in_a_β2_glycoprotein_I_induced_preeclampsia_like_mouse_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(14)00122-2 DB - PRIME DP - Unbound Medicine ER -