TY - JOUR T1 - Neuroprotective effect of melatonin against kainic acid-induced oxidative injury in hippocampal slice culture of rats. AU - Kim,Hyung A, AU - Lee,Kyung Hee, AU - Lee,Bae Hwan, Y1 - 2014/04/09/ PY - 2013/12/25/received PY - 2014/03/24/revised PY - 2014/03/31/accepted PY - 2014/4/12/entrez PY - 2014/4/12/pubmed PY - 2015/1/20/medline SP - 5940 EP - 51 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 15 IS - 4 N2 - Endogenous melatonin is a known free radical scavenger that removes reactive oxygen species (ROS), thus, alleviating oxidative stress. The purpose of this study was to demonstrate its effect against kainic acid (KA)-induced oxidative stress in organotypic hippocampal slice cultures (OHSCs). To observe neuroprotective effects of melatonin, different concentrations (0.01, 0.1 and 1 mM) of melatonin were administrated after KA treatment for 18 h in OHSCs of rat pups. Dose-response studies showed that neuronal cell death was significantly reduced after 0.1 and 1 mΜ melatonin treatments based on propidium iodide (PI) uptake and cresyl violet staining. The dichlorofluorescein (DCF) fluorescence which indicates ROS formation decreased more in the melatonin-treated group than in the KA group. The expression of 5-lipoxigenase (5-LO) and caspase-3 were reduced in the melatonin-treated groups compared to the KA group. These results suggest that melatonin may be an effective agent against KA-induced oxidative stress in the OHSC model. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/24722567/Neuroprotective_effect_of_melatonin_against_kainic_acid_induced_oxidative_injury_in_hippocampal_slice_culture_of_rats_ DB - PRIME DP - Unbound Medicine ER -