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Genetic biodiversity of Italian olives (Olea europaea) germplasm analyzed by SSR markers.
ScientificWorldJournal. 2014; 2014:296590.S

Abstract

The olive is an important fruit species cultivated for oil and table olives in Italy and the Mediterranean basin. The conservation of cultivated plants in ex situ collections is essential for the optimal management and use of their genetic resources. The largest ex situ olive germplasm collection consists of approximately 500 Italian olive varieties and corresponding to 85% of the total Italian olive germplasm is maintained at the Consiglio per la Ricerca e sperimentazione per l'Agricoltura, Centro di Ricerca per l'Olivicoltura e l'Industria Olearia (CRA-OLI), in Italy. In this work, eleven preselected nuclear microsatellite markers were used to assess genetic diversity, population structure, and gene flows with the aim of assembling a core collection. The dendrogram obtained utilizing the unweighted pair group method highlights the presence of homonymy and synonymy in olive tree datasets analyzed in this study. 439 different unique genotype profiles were obtained with this combination of 11 loci nSSR, representing 89.8% of the varieties analyzed. The remaining 10.2% comprises different variety pairs in which both accessions are genetically indistinguishable. Clustering analysis performed using BAPS software detected seven groups in Italian olive germplasm and gene flows were determined among identified clusters. We proposed an Italian core collection of 23 olive varieties capturing all detected alleles at microsatellites. The information collected in this study regarding the CRA-OLI ex situ collection can be used for breeding programs, for germplasm conservation, and for optimizing a strategy for the management of olive gene pools.

Links

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Authors+Show Affiliations

Muzzalupo I
Consiglio per la Ricerca e Sperimentazione per l'Agricoltura, Centro di Ricerca per l'Olivicoltura e l'Industria Olearia (CRA-OLI), C.da Li Rocchi-Vermicelli, 87036 Rende, Italy.
Vendramin GG
Consiglio Nazionale delle Ricerche, Istituto di Bioscienze e BioRisorse, 50019 Sesto Fiorentino, Italy.
Chiappetta A
Università della Calabria, Dipartimento di Biologia, Ecologia e Scienza della Terra, Ponte P. Bucci, 87036 Arcavacata di Rende, Italy.

MeSH

Gene PoolGenetic VariationGenome, PlantMicrosatellite RepeatsOleaPolymorphism, Single NucleotideQuantitative Trait LociSpecies Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24723801

Citation

Muzzalupo, Innocenzo, et al. "Genetic Biodiversity of Italian Olives (Olea Europaea) Germplasm Analyzed By SSR Markers." TheScientificWorldJournal, vol. 2014, 2014, p. 296590.
Muzzalupo I, Vendramin GG, Chiappetta A. Genetic biodiversity of Italian olives (Olea europaea) germplasm analyzed by SSR markers. ScientificWorldJournal. 2014;2014:296590.
Muzzalupo, I., Vendramin, G. G., & Chiappetta, A. (2014). Genetic biodiversity of Italian olives (Olea europaea) germplasm analyzed by SSR markers. TheScientificWorldJournal, 2014, 296590. https://doi.org/10.1155/2014/296590
Muzzalupo I, Vendramin GG, Chiappetta A. Genetic Biodiversity of Italian Olives (Olea Europaea) Germplasm Analyzed By SSR Markers. ScientificWorldJournal. 2014;2014:296590. PubMed PMID: 24723801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic biodiversity of Italian olives (Olea europaea) germplasm analyzed by SSR markers. AU - Muzzalupo,Innocenzo, AU - Vendramin,Giuseppe Giovanni, AU - Chiappetta,Adriana, Y1 - 2014/02/27/ PY - 2013/12/19/received PY - 2014/01/22/accepted PY - 2014/4/12/entrez PY - 2014/4/12/pubmed PY - 2015/1/8/medline SP - 296590 EP - 296590 JF - TheScientificWorldJournal JO - ScientificWorldJournal VL - 2014 N2 - The olive is an important fruit species cultivated for oil and table olives in Italy and the Mediterranean basin. The conservation of cultivated plants in ex situ collections is essential for the optimal management and use of their genetic resources. The largest ex situ olive germplasm collection consists of approximately 500 Italian olive varieties and corresponding to 85% of the total Italian olive germplasm is maintained at the Consiglio per la Ricerca e sperimentazione per l'Agricoltura, Centro di Ricerca per l'Olivicoltura e l'Industria Olearia (CRA-OLI), in Italy. In this work, eleven preselected nuclear microsatellite markers were used to assess genetic diversity, population structure, and gene flows with the aim of assembling a core collection. The dendrogram obtained utilizing the unweighted pair group method highlights the presence of homonymy and synonymy in olive tree datasets analyzed in this study. 439 different unique genotype profiles were obtained with this combination of 11 loci nSSR, representing 89.8% of the varieties analyzed. The remaining 10.2% comprises different variety pairs in which both accessions are genetically indistinguishable. Clustering analysis performed using BAPS software detected seven groups in Italian olive germplasm and gene flows were determined among identified clusters. We proposed an Italian core collection of 23 olive varieties capturing all detected alleles at microsatellites. The information collected in this study regarding the CRA-OLI ex situ collection can be used for breeding programs, for germplasm conservation, and for optimizing a strategy for the management of olive gene pools. SN - 1537-744X UR - https://www.unboundmedicine.com/medline/citation/24723801/Genetic_biodiversity_of_Italian_olives__Olea_europaea__germplasm_analyzed_by_SSR_markers_ DB - PRIME DP - Unbound Medicine ER -