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Analytical strategies based on multiple headspace extraction for the quantitative analysis of aroma components in mushrooms.
Talanta. 2014 Jun; 123:207-17.T

Abstract

Headspace (HS) and headspace solid phase microextraction (HS-SPME) analysis by gas chromatography-mass spectrometry (GC/MS) have been found to be suitable methods for the analysis of volatile organic compounds. The objectives of this paper are to study the possibilities of multiple headspace extraction (MHE) for the quantitative determination of volatile compounds in mushroom samples and to compare the results obtained using three different sample treatment techniques. For this purpose, HS with two different injection techniques (pressure-loop system and gas-tight syringe autosampling system) and HS-SPME have been studied. Three processes were optimized for the analysis of 20 volatile compounds by experimental design technique based on Central Composite Design (CCD) and Full Factorial Design depending on the used methodology. Once the designs were finished, a trade off among optimum conditions for each compound analyzed was reached. At optimum conditions, appropriate extraction time and sample amount for the three techniques used were established. Finally, the methods were validated in terms of linearity, detection and quantitation limits and repeatability. The most suitable method was then applied to the quantitative analysis of seven mushroom samples. A detailed comparison of the analytical performance characteristics of HS and HS-SPME as sample treatment techniques for final GC/MS determination is given. In addition, MHE has been proved to be an adequate technique to avoid matrix effects in complex samples quantitation. Its applicability to the determination of volatile mushroom components, along with its limitations, is discussed in this work.

Authors+Show Affiliations

Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country/EHU, P.O. Box 644, 48080 Bilbao, Spain. Electronic address: itxasosanroman@hotmail.com.Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country/EHU, P.O. Box 644, 48080 Bilbao, Spain.Central Analysis Service (SGIker), Faculty of Science and Technology, University of the Basque Country/EHU, P.O. Box 644, 48080 Bilbao, Spain.Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country/EHU, P.O. Box 644, 48080 Bilbao, Spain.DTS-OABE Company, Orozko, Biscay, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24725884

Citation

San Román, I, et al. "Analytical Strategies Based On Multiple Headspace Extraction for the Quantitative Analysis of Aroma Components in Mushrooms." Talanta, vol. 123, 2014, pp. 207-17.
San Román I, Alonso ML, Bartolomé L, et al. Analytical strategies based on multiple headspace extraction for the quantitative analysis of aroma components in mushrooms. Talanta. 2014;123:207-17.
San Román, I., Alonso, M. L., Bartolomé, L., Alonso, R. M., & Fañanás, R. (2014). Analytical strategies based on multiple headspace extraction for the quantitative analysis of aroma components in mushrooms. Talanta, 123, 207-17. https://doi.org/10.1016/j.talanta.2014.01.021
San Román I, et al. Analytical Strategies Based On Multiple Headspace Extraction for the Quantitative Analysis of Aroma Components in Mushrooms. Talanta. 2014;123:207-17. PubMed PMID: 24725884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analytical strategies based on multiple headspace extraction for the quantitative analysis of aroma components in mushrooms. AU - San Román,I, AU - Alonso,M L, AU - Bartolomé,L, AU - Alonso,R M, AU - Fañanás,R, Y1 - 2014/02/06/ PY - 2013/10/29/received PY - 2014/01/13/revised PY - 2014/01/20/accepted PY - 2014/4/15/entrez PY - 2014/4/15/pubmed PY - 2015/4/22/medline KW - GC/MS KW - HS-SPME KW - Headspace analysis KW - Multiple headspace extraction KW - Mushroom KW - Volatile compounds SP - 207 EP - 17 JF - Talanta JO - Talanta VL - 123 N2 - Headspace (HS) and headspace solid phase microextraction (HS-SPME) analysis by gas chromatography-mass spectrometry (GC/MS) have been found to be suitable methods for the analysis of volatile organic compounds. The objectives of this paper are to study the possibilities of multiple headspace extraction (MHE) for the quantitative determination of volatile compounds in mushroom samples and to compare the results obtained using three different sample treatment techniques. For this purpose, HS with two different injection techniques (pressure-loop system and gas-tight syringe autosampling system) and HS-SPME have been studied. Three processes were optimized for the analysis of 20 volatile compounds by experimental design technique based on Central Composite Design (CCD) and Full Factorial Design depending on the used methodology. Once the designs were finished, a trade off among optimum conditions for each compound analyzed was reached. At optimum conditions, appropriate extraction time and sample amount for the three techniques used were established. Finally, the methods were validated in terms of linearity, detection and quantitation limits and repeatability. The most suitable method was then applied to the quantitative analysis of seven mushroom samples. A detailed comparison of the analytical performance characteristics of HS and HS-SPME as sample treatment techniques for final GC/MS determination is given. In addition, MHE has been proved to be an adequate technique to avoid matrix effects in complex samples quantitation. Its applicability to the determination of volatile mushroom components, along with its limitations, is discussed in this work. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/24725884/Analytical_strategies_based_on_multiple_headspace_extraction_for_the_quantitative_analysis_of_aroma_components_in_mushrooms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(14)00032-0 DB - PRIME DP - Unbound Medicine ER -