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Evaluation of the antigenic relatedness and cross-protective immunity of the neuraminidase between human influenza A (H1N1) virus and highly pathogenic avian influenza A (H5N1) virus.
Virology. 2014 Apr; 454-455:169-75.V

Abstract

To determine the genetic and antigenic relatedness as well as the cross-protective immunity of human H1N1 and avian H5N1 influenza virus neuraminidase (NA), we immunized rabbits with either a baculovirus-expressed recombinant NA from A/Beijing/262/95 (BJ/262) H1N1 or A/Hong Kong/483/97 (HK/483) H5N1 virus. Cross-reactive antibody responses were evaluated by multiple serological assays and cross-protection against H5N1 virus challenge was evaluated in mice. In a neuraminidase inhibition (NI) test, the antisera exhibited substantial inhibition of NA activity of the homologous virus, but failed to inhibit the NA activity of heterologous virus. However, these antisera exhibited low levels of cross-reactivity measured by plaque size reduction, replication inhibition, single radial hemolysis, and ELISA assays. Passive immunization with HK/483 NA-specific antisera significantly reduced virus replication and disease, and afforded almost complete protection against lethal homologous virus challenge in mice. However, passive immunization with BJ/262 (H1N1) NA-specific antisera was ineffective at providing cross-protection against lethal H5N1 virus challenge and only slightly reduced weight loss. Substantial amino acid variation among the NA antigenic sites was observed between BJ/262 and HK/483 virus, which was consistent with the lack of cross-reactive NI activity by the antibody and limited cross-protective immunity in mice. These results show a strong correlation between the lack of cross-protective immunity and low structural similarities of NA from a human seasonal H1N1 virus and an avian H5N1 influenza virus.

Authors+Show Affiliations

Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.Influenza Division, National Center for Immunization and Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. Electronic address: TTumpey@cdc.gov.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24725943

Citation

Lu, Xiuhua, et al. "Evaluation of the Antigenic Relatedness and Cross-protective Immunity of the Neuraminidase Between Human Influenza a (H1N1) Virus and Highly Pathogenic Avian Influenza a (H5N1) Virus." Virology, vol. 454-455, 2014, pp. 169-75.
Lu X, Liu F, Zeng H, et al. Evaluation of the antigenic relatedness and cross-protective immunity of the neuraminidase between human influenza A (H1N1) virus and highly pathogenic avian influenza A (H5N1) virus. Virology. 2014;454-455:169-75.
Lu, X., Liu, F., Zeng, H., Sheu, T., Achenbach, J. E., Veguilla, V., Gubareva, L. V., Garten, R., Smith, C., Yang, H., Stevens, J., Xu, X., Katz, J. M., & Tumpey, T. M. (2014). Evaluation of the antigenic relatedness and cross-protective immunity of the neuraminidase between human influenza A (H1N1) virus and highly pathogenic avian influenza A (H5N1) virus. Virology, 454-455, 169-75. https://doi.org/10.1016/j.virol.2014.02.011
Lu X, et al. Evaluation of the Antigenic Relatedness and Cross-protective Immunity of the Neuraminidase Between Human Influenza a (H1N1) Virus and Highly Pathogenic Avian Influenza a (H5N1) Virus. Virology. 2014;454-455:169-75. PubMed PMID: 24725943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the antigenic relatedness and cross-protective immunity of the neuraminidase between human influenza A (H1N1) virus and highly pathogenic avian influenza A (H5N1) virus. AU - Lu,Xiuhua, AU - Liu,Feng, AU - Zeng,Hui, AU - Sheu,Tiffany, AU - Achenbach,Jenna E, AU - Veguilla,Vic, AU - Gubareva,Larisa V, AU - Garten,Rebecca, AU - Smith,Catherine, AU - Yang,Hua, AU - Stevens,James, AU - Xu,Xiyan, AU - Katz,Jacqueline M, AU - Tumpey,Terrence M, Y1 - 2014/03/05/ PY - 2013/12/31/received PY - 2014/01/29/revised PY - 2014/02/10/accepted PY - 2014/4/15/entrez PY - 2014/4/15/pubmed PY - 2014/6/3/medline KW - Anti-neuraminidase antibodies KW - Antigenic and genetic analyses KW - BALB/c mice KW - Cross-protective immunity KW - Influenza virus KW - Passive immunization SP - 169 EP - 75 JF - Virology JO - Virology VL - 454-455 N2 - To determine the genetic and antigenic relatedness as well as the cross-protective immunity of human H1N1 and avian H5N1 influenza virus neuraminidase (NA), we immunized rabbits with either a baculovirus-expressed recombinant NA from A/Beijing/262/95 (BJ/262) H1N1 or A/Hong Kong/483/97 (HK/483) H5N1 virus. Cross-reactive antibody responses were evaluated by multiple serological assays and cross-protection against H5N1 virus challenge was evaluated in mice. In a neuraminidase inhibition (NI) test, the antisera exhibited substantial inhibition of NA activity of the homologous virus, but failed to inhibit the NA activity of heterologous virus. However, these antisera exhibited low levels of cross-reactivity measured by plaque size reduction, replication inhibition, single radial hemolysis, and ELISA assays. Passive immunization with HK/483 NA-specific antisera significantly reduced virus replication and disease, and afforded almost complete protection against lethal homologous virus challenge in mice. However, passive immunization with BJ/262 (H1N1) NA-specific antisera was ineffective at providing cross-protection against lethal H5N1 virus challenge and only slightly reduced weight loss. Substantial amino acid variation among the NA antigenic sites was observed between BJ/262 and HK/483 virus, which was consistent with the lack of cross-reactive NI activity by the antibody and limited cross-protective immunity in mice. These results show a strong correlation between the lack of cross-protective immunity and low structural similarities of NA from a human seasonal H1N1 virus and an avian H5N1 influenza virus. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/24725943/Evaluation_of_the_antigenic_relatedness_and_cross_protective_immunity_of_the_neuraminidase_between_human_influenza_A__H1N1__virus_and_highly_pathogenic_avian_influenza_A__H5N1__virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(14)00055-5 DB - PRIME DP - Unbound Medicine ER -