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Osthole prevents intestinal ischemia-reperfusion-induced lung injury in a rodent model.
J Surg Res. 2014 Jun 15; 189(2):285-94.JS

Abstract

BACKGROUND

Intestinal ischemia-reperfusion (II/R) is associated with high morbidity and mortality. The aim of this study was to investigate the effects of osthole on lung injury and mortality induced by II/R.

METHODS

A rat model of II/R was induced by clamping the superior mesenteric artery for 90 min followed by reperfusion for 240 min. Osthole was administrated intraperitoneally at 30 min before intestinal ischemia (10 or 50 mg/kg). The survival rate and mean arterial pressure were observed. Blood samples were obtained for blood gas analyses. Lung injury was assessed by the histopathologic changes (hematoxylin and eosin staining), lung wet-to-dry weight ratio, and pulmonary permeability index. The levels of reactive oxygen species, malondialdehyde, interleukin 6, and tumor necrosis factor α, as well as the activities of superoxide dismutase and myeloperoxidase in lung were measured.

RESULTS

The survival rate, ratio of arterial oxygen tension to fraction of inspired oxygen, and mean arterial pressure decreased significantly after II/R. Results also indicated that II/R-induced severe lung injury evidenced by increase in pathologic scores, lung wet-to-dry weight ratio, and pulmonary permeability index, which was accompanied by increases in the levels of pulmonary reactive oxygen species, malondialdehyde, interleukin 6, tumor necrosis factor α, and the pulmonary myeloperoxidase activity and a decrease in superoxide dismutase activity. Osthole could significantly ameliorate lung injury and improve the previously mentioned variables.

CONCLUSIONS

These findings indicated that osthole could attenuate the lung injury induced by II/R in rats, at least in part, by inhibiting inflammatory response and oxidative stress.

Authors+Show Affiliations

Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.Department of Traditional Chinese Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China. Electronic address: zhoujun800615@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24726060

Citation

Mo, Li-Qun, et al. "Osthole Prevents Intestinal Ischemia-reperfusion-induced Lung Injury in a Rodent Model." The Journal of Surgical Research, vol. 189, no. 2, 2014, pp. 285-94.
Mo LQ, Chen Y, Song L, et al. Osthole prevents intestinal ischemia-reperfusion-induced lung injury in a rodent model. J Surg Res. 2014;189(2):285-94.
Mo, L. Q., Chen, Y., Song, L., Wu, G. M., Tang, N., Zhang, Y. Y., Wang, X. B., Liu, K. X., & Zhou, J. (2014). Osthole prevents intestinal ischemia-reperfusion-induced lung injury in a rodent model. The Journal of Surgical Research, 189(2), 285-94. https://doi.org/10.1016/j.jss.2014.03.026
Mo LQ, et al. Osthole Prevents Intestinal Ischemia-reperfusion-induced Lung Injury in a Rodent Model. J Surg Res. 2014 Jun 15;189(2):285-94. PubMed PMID: 24726060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Osthole prevents intestinal ischemia-reperfusion-induced lung injury in a rodent model. AU - Mo,Li-Qun, AU - Chen,Ye, AU - Song,Li, AU - Wu,Gang-Ming, AU - Tang,Ni, AU - Zhang,Ying-Ying, AU - Wang,Xiao-Bin, AU - Liu,Ke-Xuan, AU - Zhou,Jun, Y1 - 2014/03/15/ PY - 2013/11/23/received PY - 2014/02/09/revised PY - 2014/03/11/accepted PY - 2014/4/15/entrez PY - 2014/4/15/pubmed PY - 2014/8/8/medline KW - Inflammatory response KW - Intestinal ischemia–reperfusion KW - Lung injury KW - Osthole KW - Oxidative stress SP - 285 EP - 94 JF - The Journal of surgical research JO - J Surg Res VL - 189 IS - 2 N2 - BACKGROUND: Intestinal ischemia-reperfusion (II/R) is associated with high morbidity and mortality. The aim of this study was to investigate the effects of osthole on lung injury and mortality induced by II/R. METHODS: A rat model of II/R was induced by clamping the superior mesenteric artery for 90 min followed by reperfusion for 240 min. Osthole was administrated intraperitoneally at 30 min before intestinal ischemia (10 or 50 mg/kg). The survival rate and mean arterial pressure were observed. Blood samples were obtained for blood gas analyses. Lung injury was assessed by the histopathologic changes (hematoxylin and eosin staining), lung wet-to-dry weight ratio, and pulmonary permeability index. The levels of reactive oxygen species, malondialdehyde, interleukin 6, and tumor necrosis factor α, as well as the activities of superoxide dismutase and myeloperoxidase in lung were measured. RESULTS: The survival rate, ratio of arterial oxygen tension to fraction of inspired oxygen, and mean arterial pressure decreased significantly after II/R. Results also indicated that II/R-induced severe lung injury evidenced by increase in pathologic scores, lung wet-to-dry weight ratio, and pulmonary permeability index, which was accompanied by increases in the levels of pulmonary reactive oxygen species, malondialdehyde, interleukin 6, tumor necrosis factor α, and the pulmonary myeloperoxidase activity and a decrease in superoxide dismutase activity. Osthole could significantly ameliorate lung injury and improve the previously mentioned variables. CONCLUSIONS: These findings indicated that osthole could attenuate the lung injury induced by II/R in rats, at least in part, by inhibiting inflammatory response and oxidative stress. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/24726060/Osthole_prevents_intestinal_ischemia_reperfusion_induced_lung_injury_in_a_rodent_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(14)00256-X DB - PRIME DP - Unbound Medicine ER -