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Effects of resveratrol and other wine polyphenols on the proliferation, apoptosis and androgen receptor expression in LNCaP cells.
Actas Urol Esp. 2014 Jul-Aug; 38(6):397-404.AU

Abstract

PURPOSE

To address the effect of resveratrol and other red wine polyphenols on cell proliferation, apoptosis and androgen receptor (AR) expression in human prostate cancer LNCaP cells.

MATERIALS AND METHODS

LNCaP cells (5 × 102) were cultured in microtiter plate modules and treated with gallic acid, tannic acid and quercetin (1, 5 and 10 μM), rutin and morin (25, 50 and 75 μM) and resveratrol (5, 10 and 25 μM). To address the extent of proliferation at 24, 48, 72 and 96 hours, a colorimetric immunoassay method was used. An activity caspase 3/7 detection assay was used to disclose apoptosis at 24, 48 and 72 hours. AR mARN levels were determined by real time RT-PCR.

RESULTS

All polyphenols studied significantly inhibited (P<.05) cell proliferation compared to control. However, there were moderate differences between them. Resveratrol was the strongest inhibitor at different times and doses. Also, caspase-3 and caspase-7 activity was significantly higher (P<.05) than control in the presence of all the compounds, but the earlier response was achieved by resveratrol. Resveratrol, quercetin and morin were the only nutrients that significantly inhibited AR mRNA expression. Again resveratrol produced the highest inhibition (90-250 times less than control), followed by morin (67-100 times) and quercetin (55-91 times).

CONCLUSIONS

All polyphenols studied showed important antiproliferative effects and induced apoptosis when added to LNCaP cells culture. We confirm that resveratrol, morin and quercetin may achieve such effect through reduced expression of AR. The synergistic effects of these compounds and their potential to prevent progression of hormone-dependent prostate cancer merit further study.

Authors+Show Affiliations

Unidad de Investigación, Fundación para la Investigación Biomédica, Hospital Universitario de Getafe, Madrid, España.Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España.Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España.Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España.Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España.Servicio de Urología, Hospital Universitario de Getafe, Departamento Clínico, Facultad de Ciencias Biomédicas, Universidad Europea de Madrid, Madrid, España. Electronic address: javier.angulo@salud.madrid.org.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng spa

PubMed ID

24726691

Citation

Ferruelo, A, et al. "Effects of Resveratrol and Other Wine Polyphenols On the Proliferation, Apoptosis and Androgen Receptor Expression in LNCaP Cells." Actas Urologicas Espanolas, vol. 38, no. 6, 2014, pp. 397-404.
Ferruelo A, Romero I, Cabrera PM, et al. Effects of resveratrol and other wine polyphenols on the proliferation, apoptosis and androgen receptor expression in LNCaP cells. Actas Urol Esp. 2014;38(6):397-404.
Ferruelo, A., Romero, I., Cabrera, P. M., Arance, I., Andrés, G., & Angulo, J. C. (2014). Effects of resveratrol and other wine polyphenols on the proliferation, apoptosis and androgen receptor expression in LNCaP cells. Actas Urologicas Espanolas, 38(6), 397-404. https://doi.org/10.1016/j.acuro.2014.02.012
Ferruelo A, et al. Effects of Resveratrol and Other Wine Polyphenols On the Proliferation, Apoptosis and Androgen Receptor Expression in LNCaP Cells. Actas Urol Esp. 2014 Jul-Aug;38(6):397-404. PubMed PMID: 24726691.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of resveratrol and other wine polyphenols on the proliferation, apoptosis and androgen receptor expression in LNCaP cells. AU - Ferruelo,A, AU - Romero,I, AU - Cabrera,P M, AU - Arance,I, AU - Andrés,G, AU - Angulo,J C, Y1 - 2014/04/13/ PY - 2014/02/10/received PY - 2014/02/19/accepted PY - 2014/4/15/entrez PY - 2014/4/15/pubmed PY - 2015/12/29/medline KW - Androgen receptor KW - Apoptosis KW - Cell proliferation KW - LNCaP KW - LNCaP, Resveratrol KW - Polifenoles KW - Polyphenols KW - Proliferación celular KW - Receptor androgénico KW - Resveratrol SP - 397 EP - 404 JF - Actas urologicas espanolas JO - Actas Urol Esp VL - 38 IS - 6 N2 - PURPOSE: To address the effect of resveratrol and other red wine polyphenols on cell proliferation, apoptosis and androgen receptor (AR) expression in human prostate cancer LNCaP cells. MATERIALS AND METHODS: LNCaP cells (5 × 102) were cultured in microtiter plate modules and treated with gallic acid, tannic acid and quercetin (1, 5 and 10 μM), rutin and morin (25, 50 and 75 μM) and resveratrol (5, 10 and 25 μM). To address the extent of proliferation at 24, 48, 72 and 96 hours, a colorimetric immunoassay method was used. An activity caspase 3/7 detection assay was used to disclose apoptosis at 24, 48 and 72 hours. AR mARN levels were determined by real time RT-PCR. RESULTS: All polyphenols studied significantly inhibited (P<.05) cell proliferation compared to control. However, there were moderate differences between them. Resveratrol was the strongest inhibitor at different times and doses. Also, caspase-3 and caspase-7 activity was significantly higher (P<.05) than control in the presence of all the compounds, but the earlier response was achieved by resveratrol. Resveratrol, quercetin and morin were the only nutrients that significantly inhibited AR mRNA expression. Again resveratrol produced the highest inhibition (90-250 times less than control), followed by morin (67-100 times) and quercetin (55-91 times). CONCLUSIONS: All polyphenols studied showed important antiproliferative effects and induced apoptosis when added to LNCaP cells culture. We confirm that resveratrol, morin and quercetin may achieve such effect through reduced expression of AR. The synergistic effects of these compounds and their potential to prevent progression of hormone-dependent prostate cancer merit further study. SN - 1699-7980 UR - https://www.unboundmedicine.com/medline/citation/24726691/Effects_of_resveratrol_and_other_wine_polyphenols_on_the_proliferation_apoptosis_and_androgen_receptor_expression_in_LNCaP_cells_ L2 - https://medlineplus.gov/prostatecancer.html DB - PRIME DP - Unbound Medicine ER -