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Colorimetric detection of melamine in milk by citrate-stabilized gold nanoparticles.
Anal Biochem. 2014 Jul 01; 456:43-9.AB

Abstract

Here, we report a simple and sensitive colorimetric method for detection of melamine in milk using gold nanoparticles (AuNPs). AuNPs of 21-nm size were synthesized by the citrate reduction method. The method is based on the principle that the melamine causes the aggregation of AuNPs and, hence, the wine red color of AuNPs changes to blue or purple. This change in color can be visualized with the naked eye or an ultraviolet-visible (UV-Vis) spectrometer. Under optimized conditions, AuNPs are highly specific for melamine and can detect melamine down to a concentration of 0.05 mg L(-1).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kumar N
Dairy Chemistry Division, National Dairy Research Institute, Karnal 132001, Haryana, India. Electronic address: nkft87@gmail.com.
Seth R
Dairy Chemistry Division, National Dairy Research Institute, Karnal 132001, Haryana, India.
Kumar H
Dairy Chemistry Division, National Dairy Research Institute, Karnal 132001, Haryana, India.

MeSH

AnimalsCalibrationCitric AcidColorColorimetryFood ContaminationGoldMetal NanoparticlesMilkTriazines

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24727351

Citation

Kumar, Naveen, et al. "Colorimetric Detection of Melamine in Milk By Citrate-stabilized Gold Nanoparticles." Analytical Biochemistry, vol. 456, 2014, pp. 43-9.
Kumar N, Seth R, Kumar H. Colorimetric detection of melamine in milk by citrate-stabilized gold nanoparticles. Anal Biochem. 2014;456:43-9.
Kumar, N., Seth, R., & Kumar, H. (2014). Colorimetric detection of melamine in milk by citrate-stabilized gold nanoparticles. Analytical Biochemistry, 456, 43-9. https://doi.org/10.1016/j.ab.2014.04.002
Kumar N, Seth R, Kumar H. Colorimetric Detection of Melamine in Milk By Citrate-stabilized Gold Nanoparticles. Anal Biochem. 2014 Jul 1;456:43-9. PubMed PMID: 24727351.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Colorimetric detection of melamine in milk by citrate-stabilized gold nanoparticles. AU - Kumar,Naveen, AU - Seth,Raman, AU - Kumar,Harish, Y1 - 2014/04/13/ PY - 2013/12/20/received PY - 2014/03/27/revised PY - 2014/04/02/accepted PY - 2014/4/15/entrez PY - 2014/4/15/pubmed PY - 2015/1/6/medline KW - Gold nanoparticles KW - Limit of detection KW - Melamine KW - Milk KW - Quantification SP - 43 EP - 9 JF - Analytical biochemistry JO - Anal Biochem VL - 456 N2 - Here, we report a simple and sensitive colorimetric method for detection of melamine in milk using gold nanoparticles (AuNPs). AuNPs of 21-nm size were synthesized by the citrate reduction method. The method is based on the principle that the melamine causes the aggregation of AuNPs and, hence, the wine red color of AuNPs changes to blue or purple. This change in color can be visualized with the naked eye or an ultraviolet-visible (UV-Vis) spectrometer. Under optimized conditions, AuNPs are highly specific for melamine and can detect melamine down to a concentration of 0.05 mg L(-1). SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/24727351/Colorimetric_detection_of_melamine_in_milk_by_citrate_stabilized_gold_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -