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The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum.
Fungal Genet Biol. 2014 Jun; 67:58-70.FG

Abstract

Chitin is an essential component of the fungal cell wall and a potential target in the development of new antifungal compounds, due to its presence in fungi and not in plants or vertebrates. Chitin synthase genes (chs) constitute a complex family in filamentous fungi and are involved in fungal development, morphogenesis, pathogenesis and virulence. In this study, additional chs genes in the citrus postharvest pathogen Penicillium digitatum have been identified. Comparative analyses included each PdChs in each one of the classes I to VII previously established, and support the grouping of these into three divisions. Disruption of the gene coding PdChsVII, which contains a short version of a myosin motor domain, has been achieved by using Agrobacterium tumefaciens-mediated transformation and revealed its role in the life cycle of the fungus. Disruption strains were viable but showed reduced growth and conidia production. Moreover, Pdchs mutants developed morphological defects as balloon-like enlarged cells and increased chitin content, indicative of an altered cell wall structure. Gene disruption also increased susceptibility to antifungal compounds such as calcofluor white (CFW), sodium dodecyl sulfate (SDS), hydroxide peroxide (H2O2) and commercial fungicides, but significantly no change was observed in the sensitivity to antifungal peptides. The PdchsVII mutants were able to infect citrus fruit and produced tissue maceration, although had reduced virulence and most importantly were greatly impaired in the production of visible mycelium and conidia on the fruit.

Authors+Show Affiliations

Food Science Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address: mgandia@iata.csic.es.Food Science Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain.Food Science Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24727399

Citation

Gandía, Mónica, et al. "The Myosin Motor Domain-containing Chitin Synthase PdChsVII Is Required for Development, Cell Wall Integrity and Virulence in the Citrus Postharvest Pathogen Penicillium Digitatum." Fungal Genetics and Biology : FG & B, vol. 67, 2014, pp. 58-70.
Gandía M, Harries E, Marcos JF. The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum. Fungal Genet Biol. 2014;67:58-70.
Gandía, M., Harries, E., & Marcos, J. F. (2014). The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum. Fungal Genetics and Biology : FG & B, 67, 58-70. https://doi.org/10.1016/j.fgb.2014.04.002
Gandía M, Harries E, Marcos JF. The Myosin Motor Domain-containing Chitin Synthase PdChsVII Is Required for Development, Cell Wall Integrity and Virulence in the Citrus Postharvest Pathogen Penicillium Digitatum. Fungal Genet Biol. 2014;67:58-70. PubMed PMID: 24727399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum. AU - Gandía,Mónica, AU - Harries,Eleonora, AU - Marcos,Jose F, Y1 - 2014/04/12/ PY - 2013/11/18/received PY - 2014/03/21/revised PY - 2014/04/01/accepted PY - 2014/4/15/entrez PY - 2014/4/15/pubmed PY - 2015/4/23/medline KW - Cell wall KW - Chitin synthase KW - Citrus KW - Penicillium digitatum KW - Postharvest pathology SP - 58 EP - 70 JF - Fungal genetics and biology : FG & B JO - Fungal Genet. Biol. VL - 67 N2 - Chitin is an essential component of the fungal cell wall and a potential target in the development of new antifungal compounds, due to its presence in fungi and not in plants or vertebrates. Chitin synthase genes (chs) constitute a complex family in filamentous fungi and are involved in fungal development, morphogenesis, pathogenesis and virulence. In this study, additional chs genes in the citrus postharvest pathogen Penicillium digitatum have been identified. Comparative analyses included each PdChs in each one of the classes I to VII previously established, and support the grouping of these into three divisions. Disruption of the gene coding PdChsVII, which contains a short version of a myosin motor domain, has been achieved by using Agrobacterium tumefaciens-mediated transformation and revealed its role in the life cycle of the fungus. Disruption strains were viable but showed reduced growth and conidia production. Moreover, Pdchs mutants developed morphological defects as balloon-like enlarged cells and increased chitin content, indicative of an altered cell wall structure. Gene disruption also increased susceptibility to antifungal compounds such as calcofluor white (CFW), sodium dodecyl sulfate (SDS), hydroxide peroxide (H2O2) and commercial fungicides, but significantly no change was observed in the sensitivity to antifungal peptides. The PdchsVII mutants were able to infect citrus fruit and produced tissue maceration, although had reduced virulence and most importantly were greatly impaired in the production of visible mycelium and conidia on the fruit. SN - 1096-0937 UR - https://www.unboundmedicine.com/medline/citation/24727399/The_myosin_motor_domain_containing_chitin_synthase_PdChsVII_is_required_for_development_cell_wall_integrity_and_virulence_in_the_citrus_postharvest_pathogen_Penicillium_digitatum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1087-1845(14)00056-5 DB - PRIME DP - Unbound Medicine ER -