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Transcriptome comparison of global distinctive features between pollination and parthenocarpic fruit set reveals transcriptional phytohormone cross-talk in cucumber (Cucumis sativus L.).
Plant Cell Physiol. 2014 Jul; 55(7):1325-42.PC

Abstract

Parthenocarpy is an important trait determining yield and quality of fruit crops. However, the understanding of the mechanisms underlying parthenocarpy induction is limited. Cucumber (Cucumis sativus L.) is abundant in parthenocarpic germplasm resources and is an excellent model organism for parthenocarpy studies. In this study, the transcriptome of cucumber fruits was studied using RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) of set fruits were compared against aborted fruits. Distinctive features of parthenocarpic and pollinated fruits were revealed by combining the analysis of the transcriptome together with cytomorphological and physiological analysis. Cell division and the transcription of cell division genes were found to be more active in parthenocarpic fruit. The study also indicated that parthenocarpic fruit set is a high sugar-consuming process which is achieved via enhanced carbohydrate degradation through transcription of genes that lead to the breakdown of carbohydrates. Furthermore, the evidence provided by this work supports a hypothesis that parthenocarpic fruit set is induced by mimicking the processes of pollination/fertilization at the transcriptional level, i.e. by performing the same transcriptional patterns of genes inducing pollination and gametophyte development as in pollinated fruit. Based on the RNA-Seq and ovary transient expression results, 14 genes were predicted as putative parthenocarpic genes. The transcription analysis of these candidate genes revealed auxin, cytokinin and gibberellin cross-talk at the transcriptional level during parthenocarpic fruit set.

Authors+Show Affiliations

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaThese authors contributed equally to this work.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Shanxi Agricultural University, Shanxi 030801, ChinaThese authors contributed equally to this work.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, ChinaThese authors contributed equally to this work.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.Key Laboratory of Horticultural Crops Genetic Improvement of Ministry of Agriculture, Sino-Dutch Joint Lab of Horticultural Genomics Technology, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing 400044, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China jfchen@njau.edu.cn.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24733865

Citation

Li, Ji, et al. "Transcriptome Comparison of Global Distinctive Features Between Pollination and Parthenocarpic Fruit Set Reveals Transcriptional Phytohormone Cross-talk in Cucumber (Cucumis Sativus L.)." Plant & Cell Physiology, vol. 55, no. 7, 2014, pp. 1325-42.
Li J, Wu Z, Cui L, et al. Transcriptome comparison of global distinctive features between pollination and parthenocarpic fruit set reveals transcriptional phytohormone cross-talk in cucumber (Cucumis sativus L.). Plant Cell Physiol. 2014;55(7):1325-42.
Li, J., Wu, Z., Cui, L., Zhang, T., Guo, Q., Xu, J., Jia, L., Lou, Q., Huang, S., Li, Z., & Chen, J. (2014). Transcriptome comparison of global distinctive features between pollination and parthenocarpic fruit set reveals transcriptional phytohormone cross-talk in cucumber (Cucumis sativus L.). Plant & Cell Physiology, 55(7), 1325-42. https://doi.org/10.1093/pcp/pcu051
Li J, et al. Transcriptome Comparison of Global Distinctive Features Between Pollination and Parthenocarpic Fruit Set Reveals Transcriptional Phytohormone Cross-talk in Cucumber (Cucumis Sativus L.). Plant Cell Physiol. 2014;55(7):1325-42. PubMed PMID: 24733865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome comparison of global distinctive features between pollination and parthenocarpic fruit set reveals transcriptional phytohormone cross-talk in cucumber (Cucumis sativus L.). AU - Li,Ji, AU - Wu,Zhe, AU - Cui,Li, AU - Zhang,Tinglin, AU - Guo,Qinwei, AU - Xu,Jian, AU - Jia,Li, AU - Lou,Qunfeng, AU - Huang,Sanwen, AU - Li,Zhengguo, AU - Chen,Jinfeng, Y1 - 2014/04/14/ PY - 2014/4/16/entrez PY - 2014/4/16/pubmed PY - 2015/4/1/medline KW - Cucumber (Cucumis sativus L.) KW - Fruit set KW - Parthenocarpy KW - Transcriptome SP - 1325 EP - 42 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 55 IS - 7 N2 - Parthenocarpy is an important trait determining yield and quality of fruit crops. However, the understanding of the mechanisms underlying parthenocarpy induction is limited. Cucumber (Cucumis sativus L.) is abundant in parthenocarpic germplasm resources and is an excellent model organism for parthenocarpy studies. In this study, the transcriptome of cucumber fruits was studied using RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) of set fruits were compared against aborted fruits. Distinctive features of parthenocarpic and pollinated fruits were revealed by combining the analysis of the transcriptome together with cytomorphological and physiological analysis. Cell division and the transcription of cell division genes were found to be more active in parthenocarpic fruit. The study also indicated that parthenocarpic fruit set is a high sugar-consuming process which is achieved via enhanced carbohydrate degradation through transcription of genes that lead to the breakdown of carbohydrates. Furthermore, the evidence provided by this work supports a hypothesis that parthenocarpic fruit set is induced by mimicking the processes of pollination/fertilization at the transcriptional level, i.e. by performing the same transcriptional patterns of genes inducing pollination and gametophyte development as in pollinated fruit. Based on the RNA-Seq and ovary transient expression results, 14 genes were predicted as putative parthenocarpic genes. The transcription analysis of these candidate genes revealed auxin, cytokinin and gibberellin cross-talk at the transcriptional level during parthenocarpic fruit set. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/24733865/Transcriptome_comparison_of_global_distinctive_features_between_pollination_and_parthenocarpic_fruit_set_reveals_transcriptional_phytohormone_cross_talk_in_cucumber__Cucumis_sativus_L___ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcu051 DB - PRIME DP - Unbound Medicine ER -