Tags

Type your tag names separated by a space and hit enter

ATF-2/CREB/IRF-3-targeted anti-inflammatory activity of Korean red ginseng water extract.
J Ethnopharmacol. 2014 May 28; 154(1):218-28.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Korean Red Ginseng (KRG) is one of the representative traditional herbal medicines prepared from Panax ginseng Meyer (Araliaceae) in Korea. It has been reported that KRG exhibits a lot of different biological actions such as anti-aging, anti-fatigue, anti-stress, anti-atherosclerosis, anti-diabetic, anti-cancer, and anti-inflammatory activities. Although systematic studies have investigated how KRG is able to ameliorate various inflammatory diseases, its molecular inhibitory mechanisms had not been carried out prior to this study.

MATERIALS AND METHODS

In order to investigate these mechanisms, we evaluated the effects of a water extract of Korean Red Ginseng (KRG-WE) on the in vitro inflammatory responses of activated RAW264.7 cells, and on in vivo gastritis and peritonitis models by analyzing the activation events of inflammation-inducing transcription factors and their upstream kinases.

RESULTS

KRG-WE reduced the production of nitric oxide (NO), protected cells against NO-induced apoptosis, suppressed mRNA levels of inducible NO synthase (iNOS), cyclooxygenase (COX)-2, and interferon (IFN)-β, ameliorated EtOH/HCl-induced gastritis, and downregulated peritoneal exudate-derived NO production from lipopolysaccharide (LPS)-injected mice. The inhibition of these inflammatory responses by KRG-WE was regulated through the suppression of p38, c-Jun N-terminal kinase (JNK), and TANK-binding kinase 1 (TBK1) and by subsequent inhibition of activating transcription factor (ATF)-2, cAMP response element-binding protein (CREB), and IRF-3 activation. Of ginsensides included in this extract, interestingly, G-Rc showed the highest inhibitory potency on IRF-3-mediated luciferase activity.

CONCLUSION

These results strongly suggest that the anti-inflammatory activities of KRG-WE could be due to its inhibition of the p38/JNK/TBK1 activation pathway.

Authors+Show Affiliations

Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Republic of Korea.Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Republic of Korea.Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea.Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.Ginseng Corporation Central Research Institute, Daejeon 305-805, Republic of Korea.Metabolic Engineering Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.Department of Veterinary Physiology, College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: jhkim1@chonbuk.ac.kr.Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: jaecho@skku.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24735861

Citation

Yang, Yanyan, et al. "ATF-2/CREB/IRF-3-targeted Anti-inflammatory Activity of Korean Red Ginseng Water Extract." Journal of Ethnopharmacology, vol. 154, no. 1, 2014, pp. 218-28.
Yang Y, Yang WS, Yu T, et al. ATF-2/CREB/IRF-3-targeted anti-inflammatory activity of Korean red ginseng water extract. J Ethnopharmacol. 2014;154(1):218-28.
Yang, Y., Yang, W. S., Yu, T., Sung, G. H., Park, K. W., Yoon, K., Son, Y. J., Hwang, H., Kwak, Y. S., Lee, C. M., Rhee, M. H., Kim, J. H., & Cho, J. Y. (2014). ATF-2/CREB/IRF-3-targeted anti-inflammatory activity of Korean red ginseng water extract. Journal of Ethnopharmacology, 154(1), 218-28. https://doi.org/10.1016/j.jep.2014.04.008
Yang Y, et al. ATF-2/CREB/IRF-3-targeted Anti-inflammatory Activity of Korean Red Ginseng Water Extract. J Ethnopharmacol. 2014 May 28;154(1):218-28. PubMed PMID: 24735861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ATF-2/CREB/IRF-3-targeted anti-inflammatory activity of Korean red ginseng water extract. AU - Yang,Yanyan, AU - Yang,Woo Seok, AU - Yu,Tao, AU - Sung,Gi-Ho, AU - Park,Kye Won, AU - Yoon,Keejung, AU - Son,Young-Jin, AU - Hwang,Hyunsik, AU - Kwak,Yi-Seong, AU - Lee,Chang-Muk, AU - Rhee,Man Hee, AU - Kim,Jong-Hoon, AU - Cho,Jae Youl, Y1 - 2014/04/13/ PY - 2014/01/12/received PY - 2014/03/04/revised PY - 2014/04/04/accepted PY - 2014/4/17/entrez PY - 2014/4/17/pubmed PY - 2015/1/16/medline KW - ATF-2 KW - Anti-inflammatory activity KW - CREB KW - IRF-3 KW - Korean red ginseng KW - Panax ginseng Meyer SP - 218 EP - 28 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 154 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Korean Red Ginseng (KRG) is one of the representative traditional herbal medicines prepared from Panax ginseng Meyer (Araliaceae) in Korea. It has been reported that KRG exhibits a lot of different biological actions such as anti-aging, anti-fatigue, anti-stress, anti-atherosclerosis, anti-diabetic, anti-cancer, and anti-inflammatory activities. Although systematic studies have investigated how KRG is able to ameliorate various inflammatory diseases, its molecular inhibitory mechanisms had not been carried out prior to this study. MATERIALS AND METHODS: In order to investigate these mechanisms, we evaluated the effects of a water extract of Korean Red Ginseng (KRG-WE) on the in vitro inflammatory responses of activated RAW264.7 cells, and on in vivo gastritis and peritonitis models by analyzing the activation events of inflammation-inducing transcription factors and their upstream kinases. RESULTS: KRG-WE reduced the production of nitric oxide (NO), protected cells against NO-induced apoptosis, suppressed mRNA levels of inducible NO synthase (iNOS), cyclooxygenase (COX)-2, and interferon (IFN)-β, ameliorated EtOH/HCl-induced gastritis, and downregulated peritoneal exudate-derived NO production from lipopolysaccharide (LPS)-injected mice. The inhibition of these inflammatory responses by KRG-WE was regulated through the suppression of p38, c-Jun N-terminal kinase (JNK), and TANK-binding kinase 1 (TBK1) and by subsequent inhibition of activating transcription factor (ATF)-2, cAMP response element-binding protein (CREB), and IRF-3 activation. Of ginsensides included in this extract, interestingly, G-Rc showed the highest inhibitory potency on IRF-3-mediated luciferase activity. CONCLUSION: These results strongly suggest that the anti-inflammatory activities of KRG-WE could be due to its inhibition of the p38/JNK/TBK1 activation pathway. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24735861/ATF_2/CREB/IRF_3_targeted_anti_inflammatory_activity_of_Korean_red_ginseng_water_extract_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(14)00283-9 DB - PRIME DP - Unbound Medicine ER -