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Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico.
J Helminthol. 2015 Jul; 89(4):458-64.JH

Abstract

In central Mexico, populations of the freshwater snail Physella cubensis were infected with metacercariae from a species of Echinoparyphium (Digenea: Echinostomatidae). In the current study, we describe both larval and adult stages of this species obtained from experimental and natural infections. A total 180 snails were collected from Patzcuaro Lake, Michoacan state in central Mexico in July 2012. In the laboratory snails were placed in individual vials and exposed to light with the aim of observing emergence of cercariae. To obtain metacercariae, uninfected snails (P. cubensis) were exposed to cercariae. Chicks were infected with metacercariae to obtain adults. Nine days post-infection, eggs were recovered and incubated in tap water at room temperature to observe miracidia. Adults obtained from natural and experimentally infected hosts possess a head collar with 45 spines in two alternating rows, confirming the identification as Echinoparyphium recurvatum von Linstow 1873. To test the conspecificity of all stages, sequences of nuclear internal transcribed spacer 1 (ITS1), 5.8S and ITS2 rDNA were obtained from two adult worms recovered from chicks and also a natural avian host, the shoveler duck Anas clypeata, together with five cercarial and four metacercarial isolates from nine snails. The genetic divergence estimated among the 13 isolates was very low, ranging from 0 to 0.6%. Phylogenetic analyses inferred by maximum likelihood and Bayesian methods showed that all isolates of E. recurvatum form a single clade with strong support. The presence of E. recurvatum in P. cubensis and A. clypeata from central Mexico represents new host reports, and extends the distribution range in the Americas.

Authors+Show Affiliations

Departamento de Zoología,Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, C. P. 04510, Distrito Federal,México.Departamento de Zoología,Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, C. P. 04510, Distrito Federal,México.Departamento de Zoología,Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, C. P. 04510, Distrito Federal,México.Departamento de Zoología,Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Ciudad Universitaria, C. P. 04510, Distrito Federal,México.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24739119

Citation

Sereno-Uribe, A L., et al. "Morphological and Molecular Analyses of Larval and Adult Stages of Echinoparyphium Recurvatum Von Linstow 1873 (Digenea: Echinostomatidae) From Central Mexico." Journal of Helminthology, vol. 89, no. 4, 2015, pp. 458-64.
Sereno-Uribe AL, Pinacho-Pinacho CD, Sanchéz Cordero V, et al. Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico. J Helminthol. 2015;89(4):458-64.
Sereno-Uribe, A. L., Pinacho-Pinacho, C. D., Sanchéz Cordero, V., & García-Varela, M. (2015). Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico. Journal of Helminthology, 89(4), 458-64. https://doi.org/10.1017/S0022149X14000297
Sereno-Uribe AL, et al. Morphological and Molecular Analyses of Larval and Adult Stages of Echinoparyphium Recurvatum Von Linstow 1873 (Digenea: Echinostomatidae) From Central Mexico. J Helminthol. 2015;89(4):458-64. PubMed PMID: 24739119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico. AU - Sereno-Uribe,A L, AU - Pinacho-Pinacho,C D, AU - Sanchéz Cordero,V, AU - García-Varela,M, Y1 - 2014/04/17/ PY - 2014/4/18/entrez PY - 2014/4/18/pubmed PY - 2016/3/22/medline SP - 458 EP - 64 JF - Journal of helminthology JO - J Helminthol VL - 89 IS - 4 N2 - In central Mexico, populations of the freshwater snail Physella cubensis were infected with metacercariae from a species of Echinoparyphium (Digenea: Echinostomatidae). In the current study, we describe both larval and adult stages of this species obtained from experimental and natural infections. A total 180 snails were collected from Patzcuaro Lake, Michoacan state in central Mexico in July 2012. In the laboratory snails were placed in individual vials and exposed to light with the aim of observing emergence of cercariae. To obtain metacercariae, uninfected snails (P. cubensis) were exposed to cercariae. Chicks were infected with metacercariae to obtain adults. Nine days post-infection, eggs were recovered and incubated in tap water at room temperature to observe miracidia. Adults obtained from natural and experimentally infected hosts possess a head collar with 45 spines in two alternating rows, confirming the identification as Echinoparyphium recurvatum von Linstow 1873. To test the conspecificity of all stages, sequences of nuclear internal transcribed spacer 1 (ITS1), 5.8S and ITS2 rDNA were obtained from two adult worms recovered from chicks and also a natural avian host, the shoveler duck Anas clypeata, together with five cercarial and four metacercarial isolates from nine snails. The genetic divergence estimated among the 13 isolates was very low, ranging from 0 to 0.6%. Phylogenetic analyses inferred by maximum likelihood and Bayesian methods showed that all isolates of E. recurvatum form a single clade with strong support. The presence of E. recurvatum in P. cubensis and A. clypeata from central Mexico represents new host reports, and extends the distribution range in the Americas. SN - 1475-2697 UR - https://www.unboundmedicine.com/medline/citation/24739119/Morphological_and_molecular_analyses_of_larval_and_adult_stages_of_Echinoparyphium_recurvatum_von_Linstow_1873__Digenea:_Echinostomatidae__from_central_Mexico_ L2 - https://www.cambridge.org/core/product/identifier/S0022149X14000297/type/journal_article DB - PRIME DP - Unbound Medicine ER -