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Gibberellin application at pre-bloom in grapevines down-regulates the expressions of VvIAA9 and VvARF7, negative regulators of fruit set initiation, during parthenocarpic fruit development.
PLoS One. 2014; 9(4):e95634.Plos

Abstract

Fruit set is initiated only after fertilization and is tightly regulated primarily by gibberellins (GAs) and auxins. The application of either of these hormones induces parthenocarpy, fruit set without fertilization, but the molecular mechanism underlying this induction is poorly understood. In the present study, we have shown that the parthenocarpic fruits induced by GA application at pre-bloom result from the interaction of GA with auxin signaling. The transcriptional levels of the putative negative regulators of fruit set initiation, including Vitis auxin/indole-3-acetic acid transcription factor 9 (VvIAA9), Vitis auxin response factor 7 (VvARF7), and VvARF8 were monitored during inflorescence development in seeded diploid 'Tamnara' grapevines with or without GA application. Without GA application, VvIAA9, VvARF7, and VvARF8 were expressed at a relatively high level before full bloom, but decreased thereafter following pollination. After GA application at 14 days before full bloom (DBF); however, the expression levels of VvIAA9 and VvARF7 declined at 5 DBF prior to pollination. The effects of GA application on auxin levels or auxin signaling were also analyzed by monitoring the expression patterns of auxin biosynthesis genes and auxin-responsive genes with or without GA application. Transcription levels of the auxin biosynthesis genes Vitis anthranilate synthase β subunit (VvASB1-like), Vitis YUCCA2 (VvYUC2), and VvYUC6 were not significantly changed by GA application. However, the expressions of Vitis Gretchen Hagen3.2 (VvGH3.2) and VvGH3.3, auxin-responsive genes, were up-regulated from 2 DBF to full bloom with GA application. Furthermore, the Vitis GA signaling gene, VvDELLA was up-regulated by GA application during 12 DBF to 7 DBF, prior to down-regulation of VvIAA9 and VvARF7. These results suggest that VvIAA9 and VvARF7 are negative regulators of fruit set initiation in grapevines, and GA signaling is integrated with auxin signaling via VvDELLA during parthenocarpic fruit development in grapevines.

Authors+Show Affiliations

Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, Republic of Korea.Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, Republic of Korea; Department of Plant Science, Seoul National University, Seoul, Republic of Korea.Department of Life Sciences, The Catholic University of Korea, Bucheon, Republic of Korea.Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, Republic of Korea.Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, Republic of Korea.Department of Plant Science, Seoul National University, Seoul, Republic of Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24743886

Citation

Jung, Chan Jin, et al. "Gibberellin Application at Pre-bloom in Grapevines Down-regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, During Parthenocarpic Fruit Development." PloS One, vol. 9, no. 4, 2014, pp. e95634.
Jung CJ, Hur YY, Yu HJ, et al. Gibberellin application at pre-bloom in grapevines down-regulates the expressions of VvIAA9 and VvARF7, negative regulators of fruit set initiation, during parthenocarpic fruit development. PLoS One. 2014;9(4):e95634.
Jung, C. J., Hur, Y. Y., Yu, H. J., Noh, J. H., Park, K. S., & Lee, H. J. (2014). Gibberellin application at pre-bloom in grapevines down-regulates the expressions of VvIAA9 and VvARF7, negative regulators of fruit set initiation, during parthenocarpic fruit development. PloS One, 9(4), e95634. https://doi.org/10.1371/journal.pone.0095634
Jung CJ, et al. Gibberellin Application at Pre-bloom in Grapevines Down-regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, During Parthenocarpic Fruit Development. PLoS One. 2014;9(4):e95634. PubMed PMID: 24743886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gibberellin application at pre-bloom in grapevines down-regulates the expressions of VvIAA9 and VvARF7, negative regulators of fruit set initiation, during parthenocarpic fruit development. AU - Jung,Chan Jin, AU - Hur,Youn Young, AU - Yu,Hee-Ju, AU - Noh,Jung-Ho, AU - Park,Kyo-Sun, AU - Lee,Hee Jae, Y1 - 2014/04/17/ PY - 2014/01/19/received PY - 2014/03/28/accepted PY - 2014/4/19/entrez PY - 2014/4/20/pubmed PY - 2015/6/6/medline SP - e95634 EP - e95634 JF - PloS one JO - PLoS One VL - 9 IS - 4 N2 - Fruit set is initiated only after fertilization and is tightly regulated primarily by gibberellins (GAs) and auxins. The application of either of these hormones induces parthenocarpy, fruit set without fertilization, but the molecular mechanism underlying this induction is poorly understood. In the present study, we have shown that the parthenocarpic fruits induced by GA application at pre-bloom result from the interaction of GA with auxin signaling. The transcriptional levels of the putative negative regulators of fruit set initiation, including Vitis auxin/indole-3-acetic acid transcription factor 9 (VvIAA9), Vitis auxin response factor 7 (VvARF7), and VvARF8 were monitored during inflorescence development in seeded diploid 'Tamnara' grapevines with or without GA application. Without GA application, VvIAA9, VvARF7, and VvARF8 were expressed at a relatively high level before full bloom, but decreased thereafter following pollination. After GA application at 14 days before full bloom (DBF); however, the expression levels of VvIAA9 and VvARF7 declined at 5 DBF prior to pollination. The effects of GA application on auxin levels or auxin signaling were also analyzed by monitoring the expression patterns of auxin biosynthesis genes and auxin-responsive genes with or without GA application. Transcription levels of the auxin biosynthesis genes Vitis anthranilate synthase β subunit (VvASB1-like), Vitis YUCCA2 (VvYUC2), and VvYUC6 were not significantly changed by GA application. However, the expressions of Vitis Gretchen Hagen3.2 (VvGH3.2) and VvGH3.3, auxin-responsive genes, were up-regulated from 2 DBF to full bloom with GA application. Furthermore, the Vitis GA signaling gene, VvDELLA was up-regulated by GA application during 12 DBF to 7 DBF, prior to down-regulation of VvIAA9 and VvARF7. These results suggest that VvIAA9 and VvARF7 are negative regulators of fruit set initiation in grapevines, and GA signaling is integrated with auxin signaling via VvDELLA during parthenocarpic fruit development in grapevines. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24743886/Gibberellin_application_at_pre_bloom_in_grapevines_down_regulates_the_expressions_of_VvIAA9_and_VvARF7_negative_regulators_of_fruit_set_initiation_during_parthenocarpic_fruit_development_ L2 - https://dx.plos.org/10.1371/journal.pone.0095634 DB - PRIME DP - Unbound Medicine ER -