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Molecular investigation for bacterial and protozoan tick-borne pathogens in wild boars (Sus scrofa) from southern Germany.
Vector Borne Zoonotic Dis. 2014 May; 14(5):371-3.VB

Abstract

Wild boars (Sus scrofa) have been suggested to be involved in the enzootic cycle of the tick-borne pathogen Anaplasma phagocytophilum. This observation raises the question whether they serve as reservoir hosts for A. phagocytophilum and potentially for other tick-borne pathogens of public health relevance. The aim of this study was to investigate wild boars and their ticks from a forest site in southern Germany for the presence of A. phagocytophilum, Candidatus Neoehrlichia mikurensis, Rickettsia spp., Borrelia burgdorferi sensu lato (s.l.), Borrelia spp. of the relapsing fever group, and Babesia spp. Therefore, 24 wild boars collected from October, 2010, to February, 2013, were investigated by molecular methods. DNA of A. phagocytophilum was detected in three out of 24 (12.5%) wild boars and in four out of 16 (25%) ticks. DNA of none of the other pathogens was found in any wild boar, but Rickettsia spp., B. burgdorferi s.l., and Cand. N. mikurensis were found in one of the investigated ticks each. Sequences of the partial 16S rRNA gene of A. phagocytophilum from one spleen and two ticks showed 100% similarity to GenBank entries from human anaplasmosis cases (accession nos. U02521 and AY886761). The sequence from the third tick was 100% similar to sequences obtained from Ixodes ricinus and roe deer from the same study area previously. Detecting a potentially human pathogenic A. phagocytophilum variant in wild boar confirms previous findings and is of public health interest. To our knowledge, this is the first report of A. phagocytophilum in wild boars in Germany. Whether wild boars support the enzootic cycle of A. phagocytophilum variants involved in human disease requires further attention in future systematic studies.

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Authors+Show Affiliations

Silaghi C
Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München , Munich, Germany .
Pfister K
No affiliation info available
Overzier E
No affiliation info available

MeSH

Anaplasma phagocytophilumAnaplasmataceaeAnaplasmataceae InfectionsAnimalsArachnid VectorsBabesiaBabesiosisBase SequenceBorrelia burgdorferi GroupDNA, BacterialEhrlichiosisGermanyHumansIxodesLyme DiseaseMolecular Sequence DataRickettsiaSequence Analysis, DNASus scrofa

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24745766

Citation

Silaghi, Cornelia, et al. "Molecular Investigation for Bacterial and Protozoan Tick-borne Pathogens in Wild Boars (Sus Scrofa) From Southern Germany." Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), vol. 14, no. 5, 2014, pp. 371-3.
Silaghi C, Pfister K, Overzier E. Molecular investigation for bacterial and protozoan tick-borne pathogens in wild boars (Sus scrofa) from southern Germany. Vector Borne Zoonotic Dis. 2014;14(5):371-3.
Silaghi, C., Pfister, K., & Overzier, E. (2014). Molecular investigation for bacterial and protozoan tick-borne pathogens in wild boars (Sus scrofa) from southern Germany. Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 14(5), 371-3. https://doi.org/10.1089/vbz.2013.1495
Silaghi C, Pfister K, Overzier E. Molecular Investigation for Bacterial and Protozoan Tick-borne Pathogens in Wild Boars (Sus Scrofa) From Southern Germany. Vector Borne Zoonotic Dis. 2014;14(5):371-3. PubMed PMID: 24745766.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular investigation for bacterial and protozoan tick-borne pathogens in wild boars (Sus scrofa) from southern Germany. AU - Silaghi,Cornelia, AU - Pfister,Kurt, AU - Overzier,Evelyn, Y1 - 2014/04/18/ PY - 2014/4/22/entrez PY - 2014/4/22/pubmed PY - 2015/4/17/medline SP - 371 EP - 3 JF - Vector borne and zoonotic diseases (Larchmont, N.Y.) JO - Vector Borne Zoonotic Dis VL - 14 IS - 5 N2 - Wild boars (Sus scrofa) have been suggested to be involved in the enzootic cycle of the tick-borne pathogen Anaplasma phagocytophilum. This observation raises the question whether they serve as reservoir hosts for A. phagocytophilum and potentially for other tick-borne pathogens of public health relevance. The aim of this study was to investigate wild boars and their ticks from a forest site in southern Germany for the presence of A. phagocytophilum, Candidatus Neoehrlichia mikurensis, Rickettsia spp., Borrelia burgdorferi sensu lato (s.l.), Borrelia spp. of the relapsing fever group, and Babesia spp. Therefore, 24 wild boars collected from October, 2010, to February, 2013, were investigated by molecular methods. DNA of A. phagocytophilum was detected in three out of 24 (12.5%) wild boars and in four out of 16 (25%) ticks. DNA of none of the other pathogens was found in any wild boar, but Rickettsia spp., B. burgdorferi s.l., and Cand. N. mikurensis were found in one of the investigated ticks each. Sequences of the partial 16S rRNA gene of A. phagocytophilum from one spleen and two ticks showed 100% similarity to GenBank entries from human anaplasmosis cases (accession nos. U02521 and AY886761). The sequence from the third tick was 100% similar to sequences obtained from Ixodes ricinus and roe deer from the same study area previously. Detecting a potentially human pathogenic A. phagocytophilum variant in wild boar confirms previous findings and is of public health interest. To our knowledge, this is the first report of A. phagocytophilum in wild boars in Germany. Whether wild boars support the enzootic cycle of A. phagocytophilum variants involved in human disease requires further attention in future systematic studies. SN - 1557-7759 UR - https://www.unboundmedicine.com/medline/citation/24745766/Molecular_investigation_for_bacterial_and_protozoan_tick_borne_pathogens_in_wild_boars__Sus_scrofa__from_southern_Germany_ DB - PRIME DP - Unbound Medicine ER -