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Zamilon, a novel virophage with Mimiviridae host specificity.
PLoS One. 2014; 9(4):e94923.Plos

Abstract

Virophages, which are potentially important ecological regulators, have been discovered in association with members of the order Megavirales. Sputnik virophages target the Mimiviridae, Mavirus was identified with the Cafeteria roenbergensis virus, and virophage genomes reconstructed by metagenomic analyses may be associated with the Phycodnaviridae. Despite the fact that the Sputnik virophages were isolated with viruses belonging to group A of the Mimiviridae, they can grow in amoebae infected by Mimiviridae from groups A, B or C. In this study we describe Zamilon, the first virophage isolated with a member of group C of the Mimiviridae family. By co-culturing amoebae with purified Zamilon, we found that the virophage is able to multiply with members of groups B and C of the Mimiviridae family but not with viruses from group A. Zamilon has a 17,276 bp DNA genome that potentially encodes 20 genes. Most of these genes are closely related to genes from the Sputnik virophage, yet two are more related to Megavirus chiliensis genes, a group B Mimiviridae, and one to Moumouvirus monve transpoviron.

Authors+Show Affiliations

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France; Pôle des Maladies Infectieuses, Assistance Publique-Hôpitaux de Marseille, Marseille, France.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France; Pôle des Maladies Infectieuses, Assistance Publique-Hôpitaux de Marseille, Marseille, France.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS-IRD 7278, IFR48, Faculté de Médecine, Aix-Marseille Université, Marseille, France; Pôle des Maladies Infectieuses, Assistance Publique-Hôpitaux de Marseille, Marseille, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24747414

Citation

Gaia, Morgan, et al. "Zamilon, a Novel Virophage With Mimiviridae Host Specificity." PloS One, vol. 9, no. 4, 2014, pp. e94923.
Gaia M, Benamar S, Boughalmi M, et al. Zamilon, a novel virophage with Mimiviridae host specificity. PLoS One. 2014;9(4):e94923.
Gaia, M., Benamar, S., Boughalmi, M., Pagnier, I., Croce, O., Colson, P., Raoult, D., & La Scola, B. (2014). Zamilon, a novel virophage with Mimiviridae host specificity. PloS One, 9(4), e94923. https://doi.org/10.1371/journal.pone.0094923
Gaia M, et al. Zamilon, a Novel Virophage With Mimiviridae Host Specificity. PLoS One. 2014;9(4):e94923. PubMed PMID: 24747414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Zamilon, a novel virophage with Mimiviridae host specificity. AU - Gaia,Morgan, AU - Benamar,Samia, AU - Boughalmi,Mondher, AU - Pagnier,Isabelle, AU - Croce,Olivier, AU - Colson,Philippe, AU - Raoult,Didier, AU - La Scola,Bernard, Y1 - 2014/04/18/ PY - 2013/10/15/received PY - 2014/03/20/accepted PY - 2014/4/22/entrez PY - 2014/4/22/pubmed PY - 2015/1/16/medline SP - e94923 EP - e94923 JF - PloS one JO - PLoS One VL - 9 IS - 4 N2 - Virophages, which are potentially important ecological regulators, have been discovered in association with members of the order Megavirales. Sputnik virophages target the Mimiviridae, Mavirus was identified with the Cafeteria roenbergensis virus, and virophage genomes reconstructed by metagenomic analyses may be associated with the Phycodnaviridae. Despite the fact that the Sputnik virophages were isolated with viruses belonging to group A of the Mimiviridae, they can grow in amoebae infected by Mimiviridae from groups A, B or C. In this study we describe Zamilon, the first virophage isolated with a member of group C of the Mimiviridae family. By co-culturing amoebae with purified Zamilon, we found that the virophage is able to multiply with members of groups B and C of the Mimiviridae family but not with viruses from group A. Zamilon has a 17,276 bp DNA genome that potentially encodes 20 genes. Most of these genes are closely related to genes from the Sputnik virophage, yet two are more related to Megavirus chiliensis genes, a group B Mimiviridae, and one to Moumouvirus monve transpoviron. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24747414/Zamilon_a_novel_virophage_with_Mimiviridae_host_specificity_ L2 - https://dx.plos.org/10.1371/journal.pone.0094923 DB - PRIME DP - Unbound Medicine ER -