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Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome.
Gen Comp Endocrinol. 2014 Jun 01; 202:76-86.GC

Abstract

Neurochemical signaling is a major component of physiological/behavioral control throughout the animal kingdom. Gas transmitters are perhaps the most ancient class of molecules used by nervous systems for chemical communication. Three gases are generally recognized as being produced by neurons: nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). As part of an ongoing effort to identify and characterize the neurochemical signaling systems of the copepod Calanus finmarchicus, the biomass dominant zooplankton in much of the North Atlantic Ocean, we have mined a de novo assembled transcriptome for sequences encoding the neuronal biosynthetic enzymes of these gases, i.e. nitric oxide synthase (NOS), heme oxygenase (HO) and cystathionine β-synthase (CBS), respectively. Using Drosophila proteins as queries, two NOS-, one HO-, and one CBS-encoding transcripts were identified. Reverse BLAST and structural analyses of the deduced proteins suggest that each is a true member of its respective enzyme family. RNA-Seq data collected from embryos, early nauplii, late nauplii, early copepodites, late copepodites and adults revealed the expression of each transcript to be stage specific: one NOS restricted primarily to the embryo and the other was absent in the embryo but expressed in all other stages, no CBS expression in the embryo, but present in all other stages, and HO expressed across all developmental stages. Given the importance of gas transmitters in the regulatory control of a number of physiological processes, these data open opportunities for investigating the roles these proteins play under different life-stage and environmental conditions in this ecologically important species.

Authors+Show Affiliations

Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA. Electronic address: crabman@pbrc.hawaii.edu.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24747481

Citation

Christie, Andrew E., et al. "Diffusible Gas Transmitter Signaling in the Copepod Crustacean Calanus Finmarchicus: Identification of the Biosynthetic Enzymes of Nitric Oxide (NO), Carbon Monoxide (CO) and Hydrogen Sulfide (H2S) Using a De Novo Assembled Transcriptome." General and Comparative Endocrinology, vol. 202, 2014, pp. 76-86.
Christie AE, Fontanilla TM, Roncalli V, et al. Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome. Gen Comp Endocrinol. 2014;202:76-86.
Christie, A. E., Fontanilla, T. M., Roncalli, V., Cieslak, M. C., & Lenz, P. H. (2014). Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome. General and Comparative Endocrinology, 202, 76-86. https://doi.org/10.1016/j.ygcen.2014.04.003
Christie AE, et al. Diffusible Gas Transmitter Signaling in the Copepod Crustacean Calanus Finmarchicus: Identification of the Biosynthetic Enzymes of Nitric Oxide (NO), Carbon Monoxide (CO) and Hydrogen Sulfide (H2S) Using a De Novo Assembled Transcriptome. Gen Comp Endocrinol. 2014 Jun 1;202:76-86. PubMed PMID: 24747481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome. AU - Christie,Andrew E, AU - Fontanilla,Tiana M, AU - Roncalli,Vittoria, AU - Cieslak,Matthew C, AU - Lenz,Petra H, Y1 - 2014/04/18/ PY - 2014/01/27/received PY - 2014/03/26/revised PY - 2014/04/01/accepted PY - 2014/4/22/entrez PY - 2014/4/22/pubmed PY - 2014/11/12/medline KW - Cystathionine β-synthase (CBS) KW - Heme oxygenase (HO) KW - Illumina sequencing KW - Neuromodulator KW - Neurotransmitter KW - Nitric oxide synthase (NOS) SP - 76 EP - 86 JF - General and comparative endocrinology JO - Gen Comp Endocrinol VL - 202 N2 - Neurochemical signaling is a major component of physiological/behavioral control throughout the animal kingdom. Gas transmitters are perhaps the most ancient class of molecules used by nervous systems for chemical communication. Three gases are generally recognized as being produced by neurons: nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). As part of an ongoing effort to identify and characterize the neurochemical signaling systems of the copepod Calanus finmarchicus, the biomass dominant zooplankton in much of the North Atlantic Ocean, we have mined a de novo assembled transcriptome for sequences encoding the neuronal biosynthetic enzymes of these gases, i.e. nitric oxide synthase (NOS), heme oxygenase (HO) and cystathionine β-synthase (CBS), respectively. Using Drosophila proteins as queries, two NOS-, one HO-, and one CBS-encoding transcripts were identified. Reverse BLAST and structural analyses of the deduced proteins suggest that each is a true member of its respective enzyme family. RNA-Seq data collected from embryos, early nauplii, late nauplii, early copepodites, late copepodites and adults revealed the expression of each transcript to be stage specific: one NOS restricted primarily to the embryo and the other was absent in the embryo but expressed in all other stages, no CBS expression in the embryo, but present in all other stages, and HO expressed across all developmental stages. Given the importance of gas transmitters in the regulatory control of a number of physiological processes, these data open opportunities for investigating the roles these proteins play under different life-stage and environmental conditions in this ecologically important species. SN - 1095-6840 UR - https://www.unboundmedicine.com/medline/citation/24747481/Diffusible_gas_transmitter_signaling_in_the_copepod_crustacean_Calanus_finmarchicus:_identification_of_the_biosynthetic_enzymes_of_nitric_oxide__NO__carbon_monoxide__CO__and_hydrogen_sulfide__H2S__using_a_de_novo_assembled_transcriptome_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-6480(14)00123-3 DB - PRIME DP - Unbound Medicine ER -