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Cresyl violet: a rapid, simple, easily interpretable stain for detecting Pneumocystis carinii in sputum.
South Med J. 1989 Aug; 82(8):957-9.SM

Abstract

Over a three-month period at the pathology laboratory of Jackson Memorial Hospital, 110 sputum samples from 62 hospitalized patients with suspected AIDS were examined for Pneumocystis carinii. Sputum specimens were either expectorated spontaneously (most patients) or expectorated after the inhalation of small amounts of nebulized normal saline. Each sputum sample was cytocentrifuged onto two slides. One slide was stained with Gomori methenamine-silver (GMS) and the other with cresyl violet (CV). Among the 62 study patients, 18 were proven to have no histologic evidence of P carinii pneumonia. Of the remaining 44 patients, P carinii organisms were found by GMS stain in 14 (32%) and by CV stain in 18 (41%). Among those with a positive CV stain, the diagnosis was made on the first sputum specimen in 14 patients and on the second specimen in the remaining four patients. CV stain is at least as sensitive as GMS in detecting P carinii cysts in the sputum of AIDS patients with P carinii pneumonia, and its diagnostic sensitivity may exceed 40% under field conditions. Further, CV stain is much simpler to prepare than GMS and much simpler to interpret than Giemsa. It could be easily adapted for general use to expedite the diagnosis and treatment of P carinii pneumonia.

Authors+Show Affiliations

Department of Medicine, University of Miami Medical Center, Fla.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2474858

Citation

Moas, C M., et al. "Cresyl Violet: a Rapid, Simple, Easily Interpretable Stain for Detecting Pneumocystis Carinii in Sputum." Southern Medical Journal, vol. 82, no. 8, 1989, pp. 957-9.
Moas CM, Evans DA, Stein-Streilein J, et al. Cresyl violet: a rapid, simple, easily interpretable stain for detecting Pneumocystis carinii in sputum. South Med J. 1989;82(8):957-9.
Moas, C. M., Evans, D. A., Stein-Streilein, J., Ganjei, P., & Pitchenik, A. E. (1989). Cresyl violet: a rapid, simple, easily interpretable stain for detecting Pneumocystis carinii in sputum. Southern Medical Journal, 82(8), 957-9.
Moas CM, et al. Cresyl Violet: a Rapid, Simple, Easily Interpretable Stain for Detecting Pneumocystis Carinii in Sputum. South Med J. 1989;82(8):957-9. PubMed PMID: 2474858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cresyl violet: a rapid, simple, easily interpretable stain for detecting Pneumocystis carinii in sputum. AU - Moas,C M, AU - Evans,D A, AU - Stein-Streilein,J, AU - Ganjei,P, AU - Pitchenik,A E, PY - 1989/8/1/pubmed PY - 1989/8/1/medline PY - 1989/8/1/entrez SP - 957 EP - 9 JF - Southern medical journal JO - South Med J VL - 82 IS - 8 N2 - Over a three-month period at the pathology laboratory of Jackson Memorial Hospital, 110 sputum samples from 62 hospitalized patients with suspected AIDS were examined for Pneumocystis carinii. Sputum specimens were either expectorated spontaneously (most patients) or expectorated after the inhalation of small amounts of nebulized normal saline. Each sputum sample was cytocentrifuged onto two slides. One slide was stained with Gomori methenamine-silver (GMS) and the other with cresyl violet (CV). Among the 62 study patients, 18 were proven to have no histologic evidence of P carinii pneumonia. Of the remaining 44 patients, P carinii organisms were found by GMS stain in 14 (32%) and by CV stain in 18 (41%). Among those with a positive CV stain, the diagnosis was made on the first sputum specimen in 14 patients and on the second specimen in the remaining four patients. CV stain is at least as sensitive as GMS in detecting P carinii cysts in the sputum of AIDS patients with P carinii pneumonia, and its diagnostic sensitivity may exceed 40% under field conditions. Further, CV stain is much simpler to prepare than GMS and much simpler to interpret than Giemsa. It could be easily adapted for general use to expedite the diagnosis and treatment of P carinii pneumonia. SN - 0038-4348 UR - https://www.unboundmedicine.com/medline/citation/2474858/Cresyl_violet:_a_rapid_simple_easily_interpretable_stain_for_detecting_Pneumocystis_carinii_in_sputum_ DB - PRIME DP - Unbound Medicine ER -