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The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2.
Hippocampus. 2014 Sep; 24(9):1112-9.H

Abstract

The maintenance of established memories has recently been shown to involve the stabilization of GluA2-containing AMPA receptors (GluA2/AMPARs) at postsynaptic membranes. Previous studies have suggested that N-ethylmaleimide-sensitive factor (NSF) regulates the stabilization of AMPARs at the synaptic membrane. We therefore disrupted the interaction between GluA2 and NSF in the dorsal hippocampus and examined its effect on the maintenance of object location and contextual fear memory. We used two interference peptides, pep2m and pepR845A, that have been shown to block the binding of NSF to GluA2 and reduce GluA2 synaptic content. Either peptide disrupted consolidated memory, and these effects persisted for at least 5 or 28 days after peptide administration. Following peptide administration and long-term memory disruption, rats were able to acquire new memories. Memory acquisition or consolidation was not impaired when pepR845A was given immediately before the training sessions. Blocking GluA2 endocytosis with the peptide GluA23Y prevented the memory impairment effect of pepR845A. Taken together, our results indicate that the persistence of long-term memory depends on the maintenance of a steady-state level of synaptic GluA2/AMPARs, which requires the interaction of NSF with GluA2.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Migues PV
Psychology Department, McGill University, Montreal, Quebec, Canada.
Hardt O
No affiliation info available
Finnie P
No affiliation info available
Wang YW
No affiliation info available
Nader K
No affiliation info available

MeSH

AnimalsEndocytosisFearHippocampusLearningMemory, Long-TermN-Ethylmaleimide-Sensitive ProteinsNeuropsychological TestsNootropic AgentsOligopeptidesRats, Long-EvansRats, Sprague-DawleyReceptors, AMPARecognition, PsychologySpatial Memory

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24753224

Citation

Migues, Paola Virginia, et al. "The Maintenance of Long-term Memory in the Hippocampus Depends On the Interaction Between N-ethylmaleimide-sensitive Factor and GluA2." Hippocampus, vol. 24, no. 9, 2014, pp. 1112-9.
Migues PV, Hardt O, Finnie P, et al. The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2. Hippocampus. 2014;24(9):1112-9.
Migues, P. V., Hardt, O., Finnie, P., Wang, Y. W., & Nader, K. (2014). The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2. Hippocampus, 24(9), 1112-9. https://doi.org/10.1002/hipo.22295
Migues PV, et al. The Maintenance of Long-term Memory in the Hippocampus Depends On the Interaction Between N-ethylmaleimide-sensitive Factor and GluA2. Hippocampus. 2014;24(9):1112-9. PubMed PMID: 24753224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2. AU - Migues,Paola Virginia, AU - Hardt,Oliver, AU - Finnie,Peter, AU - Wang,Yu Wang, AU - Nader,Karim, Y1 - 2014/05/02/ PY - 2014/04/18/accepted PY - 2014/4/23/entrez PY - 2014/4/23/pubmed PY - 2015/5/12/medline KW - AMPA receptor trafficking KW - GluA2 KW - NSF KW - hippocampus KW - memory maintenance SP - 1112 EP - 9 JF - Hippocampus JO - Hippocampus VL - 24 IS - 9 N2 - The maintenance of established memories has recently been shown to involve the stabilization of GluA2-containing AMPA receptors (GluA2/AMPARs) at postsynaptic membranes. Previous studies have suggested that N-ethylmaleimide-sensitive factor (NSF) regulates the stabilization of AMPARs at the synaptic membrane. We therefore disrupted the interaction between GluA2 and NSF in the dorsal hippocampus and examined its effect on the maintenance of object location and contextual fear memory. We used two interference peptides, pep2m and pepR845A, that have been shown to block the binding of NSF to GluA2 and reduce GluA2 synaptic content. Either peptide disrupted consolidated memory, and these effects persisted for at least 5 or 28 days after peptide administration. Following peptide administration and long-term memory disruption, rats were able to acquire new memories. Memory acquisition or consolidation was not impaired when pepR845A was given immediately before the training sessions. Blocking GluA2 endocytosis with the peptide GluA23Y prevented the memory impairment effect of pepR845A. Taken together, our results indicate that the persistence of long-term memory depends on the maintenance of a steady-state level of synaptic GluA2/AMPARs, which requires the interaction of NSF with GluA2. SN - 1098-1063 UR - https://www.unboundmedicine.com/medline/citation/24753224/The_maintenance_of_long_term_memory_in_the_hippocampus_depends_on_the_interaction_between_N_ethylmaleimide_sensitive_factor_and_GluA2_ DB - PRIME DP - Unbound Medicine ER -