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Au nanoparticles/PAMAM dendrimer functionalized wired ethyleneamine-viologen as highly efficient interface for ultra-sensitive α-fetoprotein electrochemical immunosensor.
Biosens Bioelectron. 2014 Sep 15; 59:389-96.BB

Abstract

In this work, a novel electrochemical immunoassay system is developed for ultrasensitive detection of cancer biomarker, α-fetoprotein (AFP). This immunosensor is constructed by covalent immobilization of polyamidoamine (PAMAM) dendrimer-encapsulated gold nanoparticles (Au-PAMAM) sensing interface on a Au electrode surface, followed by sequential covalent immobilization of ethyleneamineviologen (Vio) electrochemical redox marker and AFP monoclonal antibody (mAb) on the surface of Au-PAMAM. The Au-PAMAM nanocomposite not only led to increase the electrode surface area and accelerate the electron transfer kinetics, but also it could provide a highly stable matrix for the convenient conjugation of biomolecules. Upon immunorecognition of the immobilized AFP to its antibody, the Vio peak current decreased due to the hindered electron transfer reaction on the electrode surface. Through the differential pulse voltammetry (DPV) experiments, it is found that the proposed method could detect AFP antigen at a wide linear range (0.001-45 ng mL(-1)) and a detection limit down to 130 fg mL(-1). The immunosensor exhibited high specificity for AFP detection, extremely short incubation time (5 min), good stability and acceptable reproducibility. Moreover, the fabricated immunosensor could accurately detect AFP concentration in human serum samples demonstrated by excellent correlations with standard ELISA immunoassay. In addition, electrochemical impedance spectroscopy technique was used as an efficient alternative detection system for AFP measurement with detection limit 0.5ngmL(-1) and concentration range up to 40 ng mL(-1). The present protocol is shown to be quite promising for clinical screening of cancer biomarkers and point-of-care diagnostics applications.

Authors+Show Affiliations

Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran.Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran.Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran.Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran; Research Center for Nanotechnology, University of Kurdistan, 66177-15175 Sanandaj, Iran. Electronic address: absalimi@uok.ac.ir.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24755256

Citation

Kavosi, Begard, et al. "Au nanoparticles/PAMAM Dendrimer Functionalized Wired Ethyleneamine-viologen as Highly Efficient Interface for Ultra-sensitive Α-fetoprotein Electrochemical Immunosensor." Biosensors & Bioelectronics, vol. 59, 2014, pp. 389-96.
Kavosi B, Hallaj R, Teymourian H, et al. Au nanoparticles/PAMAM dendrimer functionalized wired ethyleneamine-viologen as highly efficient interface for ultra-sensitive α-fetoprotein electrochemical immunosensor. Biosens Bioelectron. 2014;59:389-96.
Kavosi, B., Hallaj, R., Teymourian, H., & Salimi, A. (2014). Au nanoparticles/PAMAM dendrimer functionalized wired ethyleneamine-viologen as highly efficient interface for ultra-sensitive α-fetoprotein electrochemical immunosensor. Biosensors & Bioelectronics, 59, 389-96. https://doi.org/10.1016/j.bios.2014.03.049
Kavosi B, et al. Au nanoparticles/PAMAM Dendrimer Functionalized Wired Ethyleneamine-viologen as Highly Efficient Interface for Ultra-sensitive Α-fetoprotein Electrochemical Immunosensor. Biosens Bioelectron. 2014 Sep 15;59:389-96. PubMed PMID: 24755256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Au nanoparticles/PAMAM dendrimer functionalized wired ethyleneamine-viologen as highly efficient interface for ultra-sensitive α-fetoprotein electrochemical immunosensor. AU - Kavosi,Begard, AU - Hallaj,Rahman, AU - Teymourian,Hazhir, AU - Salimi,Abdollah, Y1 - 2014/03/30/ PY - 2013/12/25/received PY - 2014/03/07/revised PY - 2014/03/24/accepted PY - 2014/4/24/entrez PY - 2014/4/24/pubmed PY - 2015/1/13/medline KW - Au nanoparticles KW - Cancer biomarker KW - Electrochemical immunosensor KW - PAMAM dendrimer KW - α-Fetoprotein (AFP) SP - 389 EP - 96 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 59 N2 - In this work, a novel electrochemical immunoassay system is developed for ultrasensitive detection of cancer biomarker, α-fetoprotein (AFP). This immunosensor is constructed by covalent immobilization of polyamidoamine (PAMAM) dendrimer-encapsulated gold nanoparticles (Au-PAMAM) sensing interface on a Au electrode surface, followed by sequential covalent immobilization of ethyleneamineviologen (Vio) electrochemical redox marker and AFP monoclonal antibody (mAb) on the surface of Au-PAMAM. The Au-PAMAM nanocomposite not only led to increase the electrode surface area and accelerate the electron transfer kinetics, but also it could provide a highly stable matrix for the convenient conjugation of biomolecules. Upon immunorecognition of the immobilized AFP to its antibody, the Vio peak current decreased due to the hindered electron transfer reaction on the electrode surface. Through the differential pulse voltammetry (DPV) experiments, it is found that the proposed method could detect AFP antigen at a wide linear range (0.001-45 ng mL(-1)) and a detection limit down to 130 fg mL(-1). The immunosensor exhibited high specificity for AFP detection, extremely short incubation time (5 min), good stability and acceptable reproducibility. Moreover, the fabricated immunosensor could accurately detect AFP concentration in human serum samples demonstrated by excellent correlations with standard ELISA immunoassay. In addition, electrochemical impedance spectroscopy technique was used as an efficient alternative detection system for AFP measurement with detection limit 0.5ngmL(-1) and concentration range up to 40 ng mL(-1). The present protocol is shown to be quite promising for clinical screening of cancer biomarkers and point-of-care diagnostics applications. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24755256/Au_nanoparticles/PAMAM_dendrimer_functionalized_wired_ethyleneamine_viologen_as_highly_efficient_interface_for_ultra_sensitive_α_fetoprotein_electrochemical_immunosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00230-9 DB - PRIME DP - Unbound Medicine ER -