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The use of an indirect fluorescent antibody test for detecting Pneumocystis carinii.
Arch Pathol Lab Med. 1989 Sep; 113(9):1062-5.AP

Abstract

Pneumocystis carinii pneumonia is a major cause of morbidity and mortality in immunocompromised patients. An indirect fluorescent antibody (IFA) test has been developed using monoclonal antibodies specific for antigens on the surface of P carinii. We tested the sensitivity and specificity of this IFA test for detecting P carinii in respiratory specimens of immunocompromised patients with pulmonary symptoms undergoing bronchoscopy. Both the bronchial wash and bronchoalveolar lavage specimens of patients with and without P carinii pneumonia were studied. The bronchoalveolar lavage and bronchial wash specimens were examined using modified Wright-Giemsa and methenamine silver stains. In addition, aliquots of the specimen were fixed and stained with IFA and read with a fluorescent microscope. Fifty-nine patients were found to have P carinii organisms. The bronchial wash specimen has been shown to be less sensitive than the bronchoalveolar lavage specimen for detecting the presence of P carinii. In the bronchial wash specimen from these 59 patients, only 60% had positive modified Wright-Giemsa stains, and 70% had positive methenamine silver stains. The IFA stain was positive in 93% of the specimens tested (significantly higher than the other two stains). There was only one false-positive IFA test result among the 54 patients tested with negative results. We found the IFA stain to be superior to conventional stains when examining less-than-adequate specimens, such as those from bronchial washes.

Authors+Show Affiliations

Department of Medicine, University of Cincinnati Medical Center, OH.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2476103

Citation

Baughman, R P., et al. "The Use of an Indirect Fluorescent Antibody Test for Detecting Pneumocystis Carinii." Archives of Pathology & Laboratory Medicine, vol. 113, no. 9, 1989, pp. 1062-5.
Baughman RP, Strohofer SS, Clinton BA, et al. The use of an indirect fluorescent antibody test for detecting Pneumocystis carinii. Arch Pathol Lab Med. 1989;113(9):1062-5.
Baughman, R. P., Strohofer, S. S., Clinton, B. A., Nickol, A. D., & Frame, P. T. (1989). The use of an indirect fluorescent antibody test for detecting Pneumocystis carinii. Archives of Pathology & Laboratory Medicine, 113(9), 1062-5.
Baughman RP, et al. The Use of an Indirect Fluorescent Antibody Test for Detecting Pneumocystis Carinii. Arch Pathol Lab Med. 1989;113(9):1062-5. PubMed PMID: 2476103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of an indirect fluorescent antibody test for detecting Pneumocystis carinii. AU - Baughman,R P, AU - Strohofer,S S, AU - Clinton,B A, AU - Nickol,A D, AU - Frame,P T, PY - 1989/9/1/pubmed PY - 1989/9/1/medline PY - 1989/9/1/entrez SP - 1062 EP - 5 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 113 IS - 9 N2 - Pneumocystis carinii pneumonia is a major cause of morbidity and mortality in immunocompromised patients. An indirect fluorescent antibody (IFA) test has been developed using monoclonal antibodies specific for antigens on the surface of P carinii. We tested the sensitivity and specificity of this IFA test for detecting P carinii in respiratory specimens of immunocompromised patients with pulmonary symptoms undergoing bronchoscopy. Both the bronchial wash and bronchoalveolar lavage specimens of patients with and without P carinii pneumonia were studied. The bronchoalveolar lavage and bronchial wash specimens were examined using modified Wright-Giemsa and methenamine silver stains. In addition, aliquots of the specimen were fixed and stained with IFA and read with a fluorescent microscope. Fifty-nine patients were found to have P carinii organisms. The bronchial wash specimen has been shown to be less sensitive than the bronchoalveolar lavage specimen for detecting the presence of P carinii. In the bronchial wash specimen from these 59 patients, only 60% had positive modified Wright-Giemsa stains, and 70% had positive methenamine silver stains. The IFA stain was positive in 93% of the specimens tested (significantly higher than the other two stains). There was only one false-positive IFA test result among the 54 patients tested with negative results. We found the IFA stain to be superior to conventional stains when examining less-than-adequate specimens, such as those from bronchial washes. SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/2476103/The_use_of_an_indirect_fluorescent_antibody_test_for_detecting_Pneumocystis_carinii_ DB - PRIME DP - Unbound Medicine ER -