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Potential toxic effect of trifloxystrobin on cellular microstructure, mRNA expression and antioxidant enzymes in Chlorella vulgaris.
Environ Toxicol Pharmacol. 2014 May; 37(3):1040-7.ET

Abstract

This study investigated the effects of trifloxystrobin that one strobilurin used widely in the world as an effective fungicidal agent to control Asian soybean rust on aquatic unicellular algae Chlorella vulgaris. We determined the potential toxic effect of trifloxystrobin on C. vulgaris, and found median inhibition concentration (IC(50)) value 255.58 (95% confidence interval, 207.81-330.29)μgL(-1). In addition, the algal cells were obviously depressed or shrunk at different concentrations by electron microscopy. In the study, a real-time polymerase chain reaction (PCR) assay showed changes in transcript abundances of three photosynthetic genes, psaB, psbC, and rbcL, and one energy gene, ATPs. The results showed that trifloxystrobin reduced the transcript abundances of the three genes and enhanced expression of ATPs after 48 and 96 h. The lowest abundances of psaB, psbC and rbcL transcripts in response to trifloxystrobin exposure were 58%, 79% and 60% of those of the control, respectively. For the potential toxic influences, trifloxystrobin could decrease the soluble protein and total antioxidant contents (T-AOC), and increase superoxide dismutase (SOD) and peroxidase (POD) activity with a gradual concentration-response relationship. Overall, the present study demonstrated that trifloxystrobin could affect the activities of antioxidant enzymes, disrupts photosynthesis in C. vulgaris, and damage cellular structure.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shen YF
College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
Liu L
College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
Gong YX
College of Veterinary Medicine, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
Zhu B
College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
Liu GL
College of Science, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
Wang GX
College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China. Electronic address: wanggaoxue@126.com.

MeSH

AcetatesAnti-Infective AgentsChlorella vulgarisChloroplast Proton-Translocating ATPasesGene Expression Regulation, PlantIminesMethacrylatesMicroscopy, Electron, ScanningOxidative StressPeroxidasesPhotosystem I Protein ComplexPhotosystem II Protein ComplexPlant ProteinsRNA, MessengerRibulose-Bisphosphate CarboxylaseStrobilurinsSuperoxide Dismutase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24762415

Citation

Shen, Yu-Feng, et al. "Potential Toxic Effect of Trifloxystrobin On Cellular Microstructure, mRNA Expression and Antioxidant Enzymes in Chlorella Vulgaris." Environmental Toxicology and Pharmacology, vol. 37, no. 3, 2014, pp. 1040-7.
Shen YF, Liu L, Gong YX, et al. Potential toxic effect of trifloxystrobin on cellular microstructure, mRNA expression and antioxidant enzymes in Chlorella vulgaris. Environ Toxicol Pharmacol. 2014;37(3):1040-7.
Shen, Y. F., Liu, L., Gong, Y. X., Zhu, B., Liu, G. L., & Wang, G. X. (2014). Potential toxic effect of trifloxystrobin on cellular microstructure, mRNA expression and antioxidant enzymes in Chlorella vulgaris. Environmental Toxicology and Pharmacology, 37(3), 1040-7. https://doi.org/10.1016/j.etap.2014.04.006
Shen YF, et al. Potential Toxic Effect of Trifloxystrobin On Cellular Microstructure, mRNA Expression and Antioxidant Enzymes in Chlorella Vulgaris. Environ Toxicol Pharmacol. 2014;37(3):1040-7. PubMed PMID: 24762415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential toxic effect of trifloxystrobin on cellular microstructure, mRNA expression and antioxidant enzymes in Chlorella vulgaris. AU - Shen,Yu-Feng, AU - Liu,Lei, AU - Gong,Yu-Xin, AU - Zhu,Bin, AU - Liu,Guang-Lu, AU - Wang,Gao-Xue, Y1 - 2014/04/13/ PY - 2013/10/30/received PY - 2014/04/03/revised PY - 2014/04/03/accepted PY - 2014/4/26/entrez PY - 2014/4/26/pubmed PY - 2015/1/30/medline KW - Chlorella vulgaris KW - Microstructure KW - Oxidative damage KW - Trifloxystrobin SP - 1040 EP - 7 JF - Environmental toxicology and pharmacology JO - Environ Toxicol Pharmacol VL - 37 IS - 3 N2 - This study investigated the effects of trifloxystrobin that one strobilurin used widely in the world as an effective fungicidal agent to control Asian soybean rust on aquatic unicellular algae Chlorella vulgaris. We determined the potential toxic effect of trifloxystrobin on C. vulgaris, and found median inhibition concentration (IC(50)) value 255.58 (95% confidence interval, 207.81-330.29)μgL(-1). In addition, the algal cells were obviously depressed or shrunk at different concentrations by electron microscopy. In the study, a real-time polymerase chain reaction (PCR) assay showed changes in transcript abundances of three photosynthetic genes, psaB, psbC, and rbcL, and one energy gene, ATPs. The results showed that trifloxystrobin reduced the transcript abundances of the three genes and enhanced expression of ATPs after 48 and 96 h. The lowest abundances of psaB, psbC and rbcL transcripts in response to trifloxystrobin exposure were 58%, 79% and 60% of those of the control, respectively. For the potential toxic influences, trifloxystrobin could decrease the soluble protein and total antioxidant contents (T-AOC), and increase superoxide dismutase (SOD) and peroxidase (POD) activity with a gradual concentration-response relationship. Overall, the present study demonstrated that trifloxystrobin could affect the activities of antioxidant enzymes, disrupts photosynthesis in C. vulgaris, and damage cellular structure. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/24762415/Potential_toxic_effect_of_trifloxystrobin_on_cellular_microstructure_mRNA_expression_and_antioxidant_enzymes_in_Chlorella_vulgaris_ DB - PRIME DP - Unbound Medicine ER -