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Signal amplification in capillary electrophoresis based chemiluminescent immunoassays by using an antibody-gold nanoparticle-DNAzyme assembly.
Talanta. 2014 Jun; 124:14-20.T

Abstract

A signal amplification strategy based on antibody-gold nanoparticle-DNAzyme assembly in capillary electrophoresis based chemiluminescent immunoassays (CE-CLIA) was developed. In this CE-CLIA, antibody-AuNP-G-quadruplex/hemin was incubated with limited amount of antigen, and the formed immunocomplex and unreacted antibody-AuNP-G-quadruplex/hemin were then separated by CE and detected by CL. Due to the strong CL catalytic ability of G-quadruplex/hemin DNAzyme and a high loading ratio of DNAzyme on each AuNP, the assay was very sensitive. By taking carbohydrate antigen 19-9 (CA19-9), one of the most important carbohydrate tumor marker as the model analyte, the proposed CE-CLIA method for CA19-9 detection showed a linear range from 0.025 to 1.00 U/mL with a detection limit of 0.016 U/mL (signal/noise=3), which was more sensitive than the methods previously reported for CA19-9 quantification. The method was applied to quantify CA19-9 in human serum samples, and analytical results were in a good agreement with those obtained by using an established ELISA method.

Authors+Show Affiliations

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. Electronic address: zhaoshulin001@163.com.Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.Department of Chemistry and Biochemistry, Jackson State University, 1400 Lynch St., Jackson, MS 39217, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24767440

Citation

Shi, Min, et al. "Signal Amplification in Capillary Electrophoresis Based Chemiluminescent Immunoassays By Using an Antibody-gold nanoparticle-DNAzyme Assembly." Talanta, vol. 124, 2014, pp. 14-20.
Shi M, Zhao S, Huang Y, et al. Signal amplification in capillary electrophoresis based chemiluminescent immunoassays by using an antibody-gold nanoparticle-DNAzyme assembly. Talanta. 2014;124:14-20.
Shi, M., Zhao, S., Huang, Y., Zhao, L., & Liu, Y. M. (2014). Signal amplification in capillary electrophoresis based chemiluminescent immunoassays by using an antibody-gold nanoparticle-DNAzyme assembly. Talanta, 124, 14-20. https://doi.org/10.1016/j.talanta.2014.02.032
Shi M, et al. Signal Amplification in Capillary Electrophoresis Based Chemiluminescent Immunoassays By Using an Antibody-gold nanoparticle-DNAzyme Assembly. Talanta. 2014;124:14-20. PubMed PMID: 24767440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Signal amplification in capillary electrophoresis based chemiluminescent immunoassays by using an antibody-gold nanoparticle-DNAzyme assembly. AU - Shi,Min, AU - Zhao,Shulin, AU - Huang,Yong, AU - Zhao,Limin, AU - Liu,Yi-Ming, Y1 - 2014/02/28/ PY - 2013/10/29/received PY - 2014/02/09/revised PY - 2014/02/15/accepted PY - 2014/4/29/entrez PY - 2014/4/29/pubmed PY - 2015/4/22/medline KW - Capillary electrophoresis KW - Carbohydrate antigen 19-9 KW - Chemiluminescent immunoassays KW - Gold nanoparticles KW - Signal amplification SP - 14 EP - 20 JF - Talanta JO - Talanta VL - 124 N2 - A signal amplification strategy based on antibody-gold nanoparticle-DNAzyme assembly in capillary electrophoresis based chemiluminescent immunoassays (CE-CLIA) was developed. In this CE-CLIA, antibody-AuNP-G-quadruplex/hemin was incubated with limited amount of antigen, and the formed immunocomplex and unreacted antibody-AuNP-G-quadruplex/hemin were then separated by CE and detected by CL. Due to the strong CL catalytic ability of G-quadruplex/hemin DNAzyme and a high loading ratio of DNAzyme on each AuNP, the assay was very sensitive. By taking carbohydrate antigen 19-9 (CA19-9), one of the most important carbohydrate tumor marker as the model analyte, the proposed CE-CLIA method for CA19-9 detection showed a linear range from 0.025 to 1.00 U/mL with a detection limit of 0.016 U/mL (signal/noise=3), which was more sensitive than the methods previously reported for CA19-9 quantification. The method was applied to quantify CA19-9 in human serum samples, and analytical results were in a good agreement with those obtained by using an established ELISA method. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/24767440/Signal_amplification_in_capillary_electrophoresis_based_chemiluminescent_immunoassays_by_using_an_antibody_gold_nanoparticle_DNAzyme_assembly_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(14)00130-1 DB - PRIME DP - Unbound Medicine ER -