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Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice.
FASEB J. 2014 Aug; 28(8):3671-8.FJ

Abstract

The ferritin core is composed of fine nanoparticulate Fe(3+) oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe(3+) polyoxohydroxide (nanoFe(3+)). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe(2+) sulfate (FeSO4), nanoFe(3+), or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe(3+) was investigated using isolated duodenal loops. Our data show that FeSO4 and nanoFe(3+) are equally bioavailable in WT mice, and at wk 8 the mean ± SEM hemoglobin increase was 18 ± 7 g/L in the FeSO4 group and 30 ± 5 g/L in the nanoFe(3+) group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe(3+) is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement.

Authors+Show Affiliations

Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK;Iron Metabolism Laboratory, Queensland Institute of Medical Research Berghofer Medical Research Institute, Brisbane, Queensland, Australia; and.Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK;Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK;Iron Metabolism Laboratory, Queensland Institute of Medical Research Berghofer Medical Research Institute, Brisbane, Queensland, Australia; and.Iron Metabolism Laboratory, Queensland Institute of Medical Research Berghofer Medical Research Institute, Brisbane, Queensland, Australia; and.Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK;Iron Metabolism Laboratory, Queensland Institute of Medical Research Berghofer Medical Research Institute, Brisbane, Queensland, Australia; and School of Chemistry and Molecular Bioscience and School of Medicine, University of Queensland, Brisbane, Queensland, Australia.Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, UK; dora.pereira@mrc-hnr.cam.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24776745

Citation

Aslam, Mohamad F., et al. "Ferroportin Mediates the Intestinal Absorption of Iron From a Nanoparticulate Ferritin Core Mimetic in Mice." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 28, no. 8, 2014, pp. 3671-8.
Aslam MF, Frazer DM, Faria N, et al. Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice. FASEB J. 2014;28(8):3671-8.
Aslam, M. F., Frazer, D. M., Faria, N., Bruggraber, S. F., Wilkins, S. J., Mirciov, C., Powell, J. J., Anderson, G. J., & Pereira, D. I. (2014). Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 28(8), 3671-8. https://doi.org/10.1096/fj.14-251520
Aslam MF, et al. Ferroportin Mediates the Intestinal Absorption of Iron From a Nanoparticulate Ferritin Core Mimetic in Mice. FASEB J. 2014;28(8):3671-8. PubMed PMID: 24776745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice. AU - Aslam,Mohamad F, AU - Frazer,David M, AU - Faria,Nuno, AU - Bruggraber,Sylvaine F A, AU - Wilkins,Sarah J, AU - Mirciov,Cornel, AU - Powell,Jonathan J, AU - Anderson,Greg J, AU - Pereira,Dora I A, Y1 - 2014/04/28/ PY - 2014/4/30/entrez PY - 2014/4/30/pubmed PY - 2014/10/7/medline KW - basolateral export KW - hepcidin KW - iron homeostasis KW - knockout mice KW - nanoiron SP - 3671 EP - 8 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J VL - 28 IS - 8 N2 - The ferritin core is composed of fine nanoparticulate Fe(3+) oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe(3+) polyoxohydroxide (nanoFe(3+)). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe(2+) sulfate (FeSO4), nanoFe(3+), or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe(3+) was investigated using isolated duodenal loops. Our data show that FeSO4 and nanoFe(3+) are equally bioavailable in WT mice, and at wk 8 the mean ± SEM hemoglobin increase was 18 ± 7 g/L in the FeSO4 group and 30 ± 5 g/L in the nanoFe(3+) group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe(3+) is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement. SN - 1530-6860 UR - https://www.unboundmedicine.com/medline/citation/24776745/Ferroportin_mediates_the_intestinal_absorption_of_iron_from_a_nanoparticulate_ferritin_core_mimetic_in_mice_ L2 - https://doi.org/10.1096/fj.14-251520 DB - PRIME DP - Unbound Medicine ER -