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Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis.
J Appl Genet. 2014 Aug; 55(3):313-8.JA

Abstract

Hybrids derived from wheat (Triticum aestivum L.) × rye (Secale cereale L.) have been widely studied because of their important roles in wheat cultivar improvement. Repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 are usually used as probes in fluorescence in situ hybridization (FISH) analysis of wheat, rye, and hybrids derived from wheat × rye. Usually, some of these repetitive sequences for FISH analysis were needed to be amplified from a bacterial plasmid, extracted from bacterial cells, and labeled by nick translation. Therefore, the conventional procedure of probe preparation using these repetitive sequences is time-consuming and labor-intensive. In this study, some appropriate oligonucleotide probes have been developed which can replace the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 in FISH analysis of wheat, rye, and hybrids derived from wheat × rye. These oligonucleotides can be synthesized easily and cheaply. Therefore, FISH analysis of wheat and hybrids derived from wheat × rye using these oligonucleotide probes becomes easier and more economical.

Authors+Show Affiliations

State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People's Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24782110

Citation

Tang, Zongxiang, et al. "Oligonucleotides Replacing the Roles of Repetitive Sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH Analysis." Journal of Applied Genetics, vol. 55, no. 3, 2014, pp. 313-8.
Tang Z, Yang Z, Fu S. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet. 2014;55(3):313-8.
Tang, Z., Yang, Z., & Fu, S. (2014). Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. Journal of Applied Genetics, 55(3), 313-8. https://doi.org/10.1007/s13353-014-0215-z
Tang Z, Yang Z, Fu S. Oligonucleotides Replacing the Roles of Repetitive Sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH Analysis. J Appl Genet. 2014;55(3):313-8. PubMed PMID: 24782110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. AU - Tang,Zongxiang, AU - Yang,Zujun, AU - Fu,Shulan, Y1 - 2014/04/30/ PY - 2014/02/27/received PY - 2014/04/04/accepted PY - 2014/04/04/revised PY - 2014/5/1/entrez PY - 2014/5/2/pubmed PY - 2015/4/7/medline SP - 313 EP - 8 JF - Journal of applied genetics JO - J Appl Genet VL - 55 IS - 3 N2 - Hybrids derived from wheat (Triticum aestivum L.) × rye (Secale cereale L.) have been widely studied because of their important roles in wheat cultivar improvement. Repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 are usually used as probes in fluorescence in situ hybridization (FISH) analysis of wheat, rye, and hybrids derived from wheat × rye. Usually, some of these repetitive sequences for FISH analysis were needed to be amplified from a bacterial plasmid, extracted from bacterial cells, and labeled by nick translation. Therefore, the conventional procedure of probe preparation using these repetitive sequences is time-consuming and labor-intensive. In this study, some appropriate oligonucleotide probes have been developed which can replace the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 in FISH analysis of wheat, rye, and hybrids derived from wheat × rye. These oligonucleotides can be synthesized easily and cheaply. Therefore, FISH analysis of wheat and hybrids derived from wheat × rye using these oligonucleotide probes becomes easier and more economical. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/24782110/Oligonucleotides_replacing_the_roles_of_repetitive_sequences_pAs1_pSc119_2_pTa_535_pTa71_CCS1_and_pAWRC_1_for_FISH_analysis_ L2 - https://dx.doi.org/10.1007/s13353-014-0215-z DB - PRIME DP - Unbound Medicine ER -