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[Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis].
Yao Xue Xue Bao. 2014 Jan; 49(1):142-7.YX

Abstract

This study is to investigate the effect of artesunate on transforming growth factor-beta1 (TGF-beta1) induced epithelial-mesenchymal transition (EMT) and its possible mechanism. After the in vitro cultured RLE-6TN cells were treated with TGF-beta1 then artesunate intervened on it, after 24 h, expression of the markers of mesenchymal cell was assayed using Western blotting and real-time PCR analysis. Western blotting was also used to detect the effect of TGF-beta1 on the Smad3 and Smad7 expressions of RLE-6TN cells. Morphological alterations were examined by phase-contrast microscope, and ultrastructure changes by electron microscope. Incubation of RLE-6TN cells with TGF-beta1 resulted in the up-regulation of the expression of the mesenchymal cell markers, after artesunate intervened on it, resulted in the down-regulation of the expression. Meanwhile, incubation with artesunate intervened on RLE-6TN cells could lead to the apparent down-regulation of the expression of Smad3 and up-regulation of Samd7 and the transition of RLE-6TN cells to mesenchymal-like by TGF-beta1 induction, after artesunate intervened on it, RLE-6TN cells to epithelial-like. TGF-beta1 induced epithelial-mesenchymal transition process; artesunate can inhibit TGF-beta1-induced epithelial-mesenchymal transition process, the possible mechanism is up-regulation of the expression of Smad7 and down-regulation of the expression of Smad3, meanwhile inhibits phosphorylation of Smad3.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

24783520

Citation

Wang, Chang-Ming, et al. "[Relationship Between Artesunate Influence On the Process of TGF-beta1 Induced Alveolar Epithelial Cells Transform Into Mesenchymal Cells and On Idiopathic Pulmonary Fibrosis]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 49, no. 1, 2014, pp. 142-7.
Wang CM, Chen J, Jiang M, et al. [Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis]. Yao Xue Xue Bao. 2014;49(1):142-7.
Wang, C. M., Chen, J., Jiang, M., Xuan, X. P., & Li, H. X. (2014). [Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 49(1), 142-7.
Wang CM, et al. [Relationship Between Artesunate Influence On the Process of TGF-beta1 Induced Alveolar Epithelial Cells Transform Into Mesenchymal Cells and On Idiopathic Pulmonary Fibrosis]. Yao Xue Xue Bao. 2014;49(1):142-7. PubMed PMID: 24783520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Relationship between artesunate influence on the process of TGF-beta1 induced alveolar epithelial cells transform into mesenchymal cells and on idiopathic pulmonary fibrosis]. AU - Wang,Chang-Ming, AU - Chen,Juan, AU - Jiang,Ming, AU - Xuan,Xiu-Ping, AU - Li,Hong-Xiu, PY - 2014/5/3/entrez PY - 2014/5/3/pubmed PY - 2016/2/3/medline SP - 142 EP - 7 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 49 IS - 1 N2 - This study is to investigate the effect of artesunate on transforming growth factor-beta1 (TGF-beta1) induced epithelial-mesenchymal transition (EMT) and its possible mechanism. After the in vitro cultured RLE-6TN cells were treated with TGF-beta1 then artesunate intervened on it, after 24 h, expression of the markers of mesenchymal cell was assayed using Western blotting and real-time PCR analysis. Western blotting was also used to detect the effect of TGF-beta1 on the Smad3 and Smad7 expressions of RLE-6TN cells. Morphological alterations were examined by phase-contrast microscope, and ultrastructure changes by electron microscope. Incubation of RLE-6TN cells with TGF-beta1 resulted in the up-regulation of the expression of the mesenchymal cell markers, after artesunate intervened on it, resulted in the down-regulation of the expression. Meanwhile, incubation with artesunate intervened on RLE-6TN cells could lead to the apparent down-regulation of the expression of Smad3 and up-regulation of Samd7 and the transition of RLE-6TN cells to mesenchymal-like by TGF-beta1 induction, after artesunate intervened on it, RLE-6TN cells to epithelial-like. TGF-beta1 induced epithelial-mesenchymal transition process; artesunate can inhibit TGF-beta1-induced epithelial-mesenchymal transition process, the possible mechanism is up-regulation of the expression of Smad7 and down-regulation of the expression of Smad3, meanwhile inhibits phosphorylation of Smad3. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/24783520/[Relationship_between_artesunate_influence_on_the_process_of_TGF_beta1_induced_alveolar_epithelial_cells_transform_into_mesenchymal_cells_and_on_idiopathic_pulmonary_fibrosis]_ L2 - https://antibodies.cancer.gov/detail/CPTC-TGFB1-1 DB - PRIME DP - Unbound Medicine ER -