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UHPLC-ESI-MS/MS determination and pharmacokinetic study of two alkaloid components in rat plasma after oral administration of the extract of Corydalis bungeana Turcz.

Abstract

Corynoline and acetycorynoline are active compounds of Corydalis bungeana Turcz. with various pharmacological effects such as sedation, anti-leptospira and liver injury protection effects. A specific, simple and sensitive UHPLC-ESI-MS/MS method was developed and validated for the pharmacokinetic study of corynoline and acetycorynoline in rat plasma. Corynoline and acetycorynoline were extracted from plasma samples by liquid-liquid extraction. The analysis was carried out on an Agilent SB-C18 column (1.8 μm, 50 mm×2.1mm) with the mobile phase of acetonitrile-0.1% formic acid (80:20, v/v). The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source in positive mode. The current UHPLC-ESI-MS/MS assay was validated for linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability. The lower limits of quantification were 0.10 ng/mL for corynoline and 0.353 ng/mL for acetycorynoline. Intra-day and inter-day precision were less than 12.3% and accuracy ranged from 0.18% to 14.9%. The mean extraction recoveries of analytes and internal standard (IS) from rat plasma were all more than 76.2%. This validated method was successfully applied to pharmacokinetic study in rats after oral administration of corynoline, acetycorynoline and the extract of Corydalis bungeana Turcz.

Authors+Show Affiliations

College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China; Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, New Brunswick, NJ 08854, USA.College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China; Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, New Brunswick, NJ 08854, USA.College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China.College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China.College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China.Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. Electronic address: liugaofengwty@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24786221

Citation

Yang, Chunjuan, et al. "UHPLC-ESI-MS/MS Determination and Pharmacokinetic Study of Two Alkaloid Components in Rat Plasma After Oral Administration of the Extract of Corydalis Bungeana Turcz." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 960, 2014, pp. 59-66.
Yang C, Xiao Y, Wang Z, et al. UHPLC-ESI-MS/MS determination and pharmacokinetic study of two alkaloid components in rat plasma after oral administration of the extract of Corydalis bungeana Turcz. J Chromatogr B Analyt Technol Biomed Life Sci. 2014;960:59-66.
Yang, C., Xiao, Y., Wang, Z., Wang, S., Chen, L., Wu, L., & Liu, G. (2014). UHPLC-ESI-MS/MS determination and pharmacokinetic study of two alkaloid components in rat plasma after oral administration of the extract of Corydalis bungeana Turcz. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 960, 59-66. https://doi.org/10.1016/j.jchromb.2014.04.009
Yang C, et al. UHPLC-ESI-MS/MS Determination and Pharmacokinetic Study of Two Alkaloid Components in Rat Plasma After Oral Administration of the Extract of Corydalis Bungeana Turcz. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jun 1;960:59-66. PubMed PMID: 24786221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - UHPLC-ESI-MS/MS determination and pharmacokinetic study of two alkaloid components in rat plasma after oral administration of the extract of Corydalis bungeana Turcz. AU - Yang,Chunjuan, AU - Xiao,Yang, AU - Wang,Zhibin, AU - Wang,Shuhong, AU - Chen,Lijuan, AU - Wu,Lijun, AU - Liu,Gaofeng, Y1 - 2014/04/18/ PY - 2013/11/20/received PY - 2014/03/06/revised PY - 2014/04/07/accepted PY - 2014/5/3/entrez PY - 2014/5/3/pubmed PY - 2015/1/13/medline KW - Acetycorynoline KW - Corydalis bungeana Turcz. KW - Corynoline KW - Pharmacokinetics KW - UHPLC–ESI–MS/MS SP - 59 EP - 66 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 960 N2 - Corynoline and acetycorynoline are active compounds of Corydalis bungeana Turcz. with various pharmacological effects such as sedation, anti-leptospira and liver injury protection effects. A specific, simple and sensitive UHPLC-ESI-MS/MS method was developed and validated for the pharmacokinetic study of corynoline and acetycorynoline in rat plasma. Corynoline and acetycorynoline were extracted from plasma samples by liquid-liquid extraction. The analysis was carried out on an Agilent SB-C18 column (1.8 μm, 50 mm×2.1mm) with the mobile phase of acetonitrile-0.1% formic acid (80:20, v/v). The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source in positive mode. The current UHPLC-ESI-MS/MS assay was validated for linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability. The lower limits of quantification were 0.10 ng/mL for corynoline and 0.353 ng/mL for acetycorynoline. Intra-day and inter-day precision were less than 12.3% and accuracy ranged from 0.18% to 14.9%. The mean extraction recoveries of analytes and internal standard (IS) from rat plasma were all more than 76.2%. This validated method was successfully applied to pharmacokinetic study in rats after oral administration of corynoline, acetycorynoline and the extract of Corydalis bungeana Turcz. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24786221/UHPLC_ESI_MS/MS_determination_and_pharmacokinetic_study_of_two_alkaloid_components_in_rat_plasma_after_oral_administration_of_the_extract_of_Corydalis_bungeana_Turcz_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(14)00240-2 DB - PRIME DP - Unbound Medicine ER -