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Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering.
Int J Nanomedicine 2014; 9:1991-2003IJ

Abstract

The aim of this study was to develop a kind of osteogenic biodegradable composite graft consisting of human placenta-derived mesenchymal stem cell (hPMSC) material for site-specific repair of bone defects and attenuation of clinical symptoms. The novel nano- to micro-structured biodegradable hybrid fibers were prepared by electrospinning. The characteristics of the hybrid membranes were investigated by a range of methods, including Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry. Morphological study with scanning electron microscopy showed that the average fiber diameter and the number of nanoscale pores on each individual fiber surface decreased with increasing concentration of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC). The prepared polylactide (PLA)/PCEC fibrous membranes favored hPMSC attachment and proliferation by providing an interconnected, porous, three-dimensional mimicked extracellular environment. What is more, hPMSCs cultured on the electrospun hybrid PLA/PCEC fibrous scaffolds could be effectively differentiated into bone-associated cells by positive alizarin red staining. Given the good cellular response and excellent osteogenic potential in vitro, the electrospun PLA/PCEC fibrous scaffolds could be one of the most promising candidates for bone tissue engineering.

Authors+Show Affiliations

State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China ; Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24790439

Citation

Wang, YueLong, et al. "Preparation and Characterization of Polylactide/poly(ε-caprolactone)-poly(ethylene Glycol)-poly(ε-caprolactone) Hybrid Fibers for Potential Application in Bone Tissue Engineering." International Journal of Nanomedicine, vol. 9, 2014, pp. 1991-2003.
Wang Y, Guo G, Chen H, et al. Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering. Int J Nanomedicine. 2014;9:1991-2003.
Wang, Y., Guo, G., Chen, H., Gao, X., Fan, R., Zhang, D., & Zhou, L. (2014). Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering. International Journal of Nanomedicine, 9, pp. 1991-2003. doi:10.2147/IJN.S55318.
Wang Y, et al. Preparation and Characterization of Polylactide/poly(ε-caprolactone)-poly(ethylene Glycol)-poly(ε-caprolactone) Hybrid Fibers for Potential Application in Bone Tissue Engineering. Int J Nanomedicine. 2014;9:1991-2003. PubMed PMID: 24790439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering. AU - Wang,YueLong, AU - Guo,Gang, AU - Chen,HaiFeng, AU - Gao,Xiang, AU - Fan,RangRang, AU - Zhang,DongMei, AU - Zhou,LiangXue, Y1 - 2014/04/17/ PY - 2014/5/3/entrez PY - 2014/5/3/pubmed PY - 2014/12/15/medline KW - PCEC KW - PLA KW - bone tissue engineering KW - electrospinning KW - hPMSCs SP - 1991 EP - 2003 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 9 N2 - The aim of this study was to develop a kind of osteogenic biodegradable composite graft consisting of human placenta-derived mesenchymal stem cell (hPMSC) material for site-specific repair of bone defects and attenuation of clinical symptoms. The novel nano- to micro-structured biodegradable hybrid fibers were prepared by electrospinning. The characteristics of the hybrid membranes were investigated by a range of methods, including Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry. Morphological study with scanning electron microscopy showed that the average fiber diameter and the number of nanoscale pores on each individual fiber surface decreased with increasing concentration of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC). The prepared polylactide (PLA)/PCEC fibrous membranes favored hPMSC attachment and proliferation by providing an interconnected, porous, three-dimensional mimicked extracellular environment. What is more, hPMSCs cultured on the electrospun hybrid PLA/PCEC fibrous scaffolds could be effectively differentiated into bone-associated cells by positive alizarin red staining. Given the good cellular response and excellent osteogenic potential in vitro, the electrospun PLA/PCEC fibrous scaffolds could be one of the most promising candidates for bone tissue engineering. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/24790439/Preparation_and_characterization_of_polylactide/poly_ε_caprolactone__poly_ethylene_glycol__poly_ε_caprolactone__hybrid_fibers_for_potential_application_in_bone_tissue_engineering_ L2 - https://dx.doi.org/10.2147/IJN.S55318 DB - PRIME DP - Unbound Medicine ER -