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Identification and characterization of suppressor mutants of spl11- mediated cell death in rice.
Mol Plant Microbe Interact. 2014 Jun; 27(6):528-36.MP

Abstract

Lesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe oryzae. Spl11 encodes an E3 ubiquitin ligase and is a negative regulator of PCD in rice. To study the regulation of Spl11-mediated PCD, we performed a genetic screen and identified three spl11 cell-death suppressor (sds) mutants. These suppressors were characterized for their resistance to X. oryzae pv. oryzae and M. oryzae and for their expression of defense-related genes. The suppression of the cell-death phenotypes was generally correlated with reduced expression of defense-related genes. When rice was challenged with avirulent isolates of M. oryzae, the disease phenotype was unaffected in the sds mutants, indicating that the suppression might be Spl11-mediated pathway specific and may only be involved in basal defense. In addition, we mapped one of the suppressor mutations to a 140-kb interval on the long arm of rice chromosome 1. Identification and characterization of these sds mutants should facilitate our efforts to elucidate the Spl11-mediated PCD pathway.

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Publisher Full Text

Authors

Shirsekar GS
No affiliation info available
Vega-Sanchez ME
No affiliation info available
Bordeos A
No affiliation info available
Baraoidan M
No affiliation info available
Swisshelm A
No affiliation info available
Fan J
No affiliation info available
Park CH
No affiliation info available
Leung H
No affiliation info available
Wang GL
No affiliation info available

MeSH

Cell DeathChromosome MappingGene Expression Regulation, PlantMagnaportheMutationOryzaPhenotypePlant DiseasesPlant ImmunityPlant LeavesPlant ProteinsUbiquitin-Protein LigasesXanthomonas

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24794921

Citation

Shirsekar, Gautam S., et al. "Identification and Characterization of Suppressor Mutants of Spl11- Mediated Cell Death in Rice." Molecular Plant-microbe Interactions : MPMI, vol. 27, no. 6, 2014, pp. 528-36.
Shirsekar GS, Vega-Sanchez ME, Bordeos A, et al. Identification and characterization of suppressor mutants of spl11- mediated cell death in rice. Mol Plant Microbe Interact. 2014;27(6):528-36.
Shirsekar, G. S., Vega-Sanchez, M. E., Bordeos, A., Baraoidan, M., Swisshelm, A., Fan, J., Park, C. H., Leung, H., & Wang, G. L. (2014). Identification and characterization of suppressor mutants of spl11- mediated cell death in rice. Molecular Plant-microbe Interactions : MPMI, 27(6), 528-36. https://doi.org/10.1094/MPMI-08-13-0259-R
Shirsekar GS, et al. Identification and Characterization of Suppressor Mutants of Spl11- Mediated Cell Death in Rice. Mol Plant Microbe Interact. 2014;27(6):528-36. PubMed PMID: 24794921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of suppressor mutants of spl11- mediated cell death in rice. AU - Shirsekar,Gautam S, AU - Vega-Sanchez,Miguel E, AU - Bordeos,Alicia, AU - Baraoidan,Marietta, AU - Swisshelm,Andrew, AU - Fan,Jiangbo, AU - Park,Chan Ho, AU - Leung,Hei, AU - Wang,Guo-Liang, PY - 2014/5/6/entrez PY - 2014/5/6/pubmed PY - 2014/8/8/medline SP - 528 EP - 36 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 27 IS - 6 N2 - Lesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe oryzae. Spl11 encodes an E3 ubiquitin ligase and is a negative regulator of PCD in rice. To study the regulation of Spl11-mediated PCD, we performed a genetic screen and identified three spl11 cell-death suppressor (sds) mutants. These suppressors were characterized for their resistance to X. oryzae pv. oryzae and M. oryzae and for their expression of defense-related genes. The suppression of the cell-death phenotypes was generally correlated with reduced expression of defense-related genes. When rice was challenged with avirulent isolates of M. oryzae, the disease phenotype was unaffected in the sds mutants, indicating that the suppression might be Spl11-mediated pathway specific and may only be involved in basal defense. In addition, we mapped one of the suppressor mutations to a 140-kb interval on the long arm of rice chromosome 1. Identification and characterization of these sds mutants should facilitate our efforts to elucidate the Spl11-mediated PCD pathway. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/24794921/Identification_and_characterization_of_suppressor_mutants_of_spl11__mediated_cell_death_in_rice_ L2 - https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-13-0259-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -