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iTRAQ-based quantitative proteomics study on the neuroprotective effects of extract of Acanthopanax senticosus harm on SH-SY5Y cells overexpressing A53T mutant α-synuclein.
Neurochem Int. 2014 Jun; 72:37-47.NI

Abstract

Extract of Acanthopanax senticosus harms (EAS) has been shown to have neuroprotective effects on Parkinson's disease (PD) cell model against α-synuclein overexpression and toxicity. However, studies of its anti-PD mechanism are challenging, owing to the complex pathophysiology of PD, and complexity of EAS with multiple constituents acting on different proteomic pathways. Here, we have investigated the proteomic profiles and potential biomarkers in a cell model of A53T mutant α-synuclein (A53T-α-Syn) overexpression after treatment of EAS. Using an iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomics research approach, we identified 3425 modulated proteins, out of which 84 were found to be altered by A53T-α-Syn and considered as potential biomarkers. After treatment with EAS, the group showed the tendency to correct the abnormal expressions of 16 proteins out of 84 potential biomarkers, which were associated with the formation of Lewy body, mitochondrial energy metabolism, protein synthesis and apoptosis, etc. This study indicated that EAS might be a promising candidate for prevention or treatment of PD by regulating the related proteomic pathways in A53T-α-Syn transgenic SH-SY5Y cells.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li XZ
Chinese Medicine Toxicological Laboratory, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
Zhang SN
Chinese Medicine Toxicological Laboratory, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
Wang KX
Chinese Medicine Toxicological Laboratory, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
Liu SM
Chinese Medicine Toxicological Laboratory, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China; Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China. Electronic address: keji-liu@163.com.
Lu F
Chinese Medicine Toxicological Laboratory, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China. Electronic address: lufang_19790501@163.com.

MeSH

Cell LineEleutherococcusEnergy MetabolismHumansMutationNeuroprotective AgentsPlant ExtractsProteomicsTransfectionalpha-Synuclein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24795107

Citation

Li, Xu-zhao, et al. "ITRAQ-based Quantitative Proteomics Study On the Neuroprotective Effects of Extract of Acanthopanax Senticosus Harm On SH-SY5Y Cells Overexpressing A53T Mutant Α-synuclein." Neurochemistry International, vol. 72, 2014, pp. 37-47.
Li XZ, Zhang SN, Wang KX, et al. ITRAQ-based quantitative proteomics study on the neuroprotective effects of extract of Acanthopanax senticosus harm on SH-SY5Y cells overexpressing A53T mutant α-synuclein. Neurochem Int. 2014;72:37-47.
Li, X. Z., Zhang, S. N., Wang, K. X., Liu, S. M., & Lu, F. (2014). ITRAQ-based quantitative proteomics study on the neuroprotective effects of extract of Acanthopanax senticosus harm on SH-SY5Y cells overexpressing A53T mutant α-synuclein. Neurochemistry International, 72, 37-47. https://doi.org/10.1016/j.neuint.2014.04.012
Li XZ, et al. ITRAQ-based Quantitative Proteomics Study On the Neuroprotective Effects of Extract of Acanthopanax Senticosus Harm On SH-SY5Y Cells Overexpressing A53T Mutant Α-synuclein. Neurochem Int. 2014;72:37-47. PubMed PMID: 24795107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - iTRAQ-based quantitative proteomics study on the neuroprotective effects of extract of Acanthopanax senticosus harm on SH-SY5Y cells overexpressing A53T mutant α-synuclein. AU - Li,Xu-zhao, AU - Zhang,Shuai-nan, AU - Wang,Ke-xin, AU - Liu,Shu-min, AU - Lu,Fang, Y1 - 2014/04/30/ PY - 2014/02/19/received PY - 2014/04/04/revised PY - 2014/04/08/accepted PY - 2014/5/6/entrez PY - 2014/5/6/pubmed PY - 2015/1/13/medline KW - A53T mutant α-synuclein KW - Acanthopanax senticosus harms KW - Parkinson’s disease KW - Proteomics KW - iTRAQ SP - 37 EP - 47 JF - Neurochemistry international JO - Neurochem Int VL - 72 N2 - Extract of Acanthopanax senticosus harms (EAS) has been shown to have neuroprotective effects on Parkinson's disease (PD) cell model against α-synuclein overexpression and toxicity. However, studies of its anti-PD mechanism are challenging, owing to the complex pathophysiology of PD, and complexity of EAS with multiple constituents acting on different proteomic pathways. Here, we have investigated the proteomic profiles and potential biomarkers in a cell model of A53T mutant α-synuclein (A53T-α-Syn) overexpression after treatment of EAS. Using an iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomics research approach, we identified 3425 modulated proteins, out of which 84 were found to be altered by A53T-α-Syn and considered as potential biomarkers. After treatment with EAS, the group showed the tendency to correct the abnormal expressions of 16 proteins out of 84 potential biomarkers, which were associated with the formation of Lewy body, mitochondrial energy metabolism, protein synthesis and apoptosis, etc. This study indicated that EAS might be a promising candidate for prevention or treatment of PD by regulating the related proteomic pathways in A53T-α-Syn transgenic SH-SY5Y cells. SN - 1872-9754 UR - https://www.unboundmedicine.com/medline/citation/24795107/iTRAQ_based_quantitative_proteomics_study_on_the_neuroprotective_effects_of_extract_of_Acanthopanax_senticosus_harm_on_SH_SY5Y_cells_overexpressing_A53T_mutant_α_synuclein_ DB - PRIME DP - Unbound Medicine ER -