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Determinants of human myelin basic protein that induce encephalitogenic T cells in Lewis rats.
J Immunol. 1989 Dec 01; 143(11):3512-6.JI

Abstract

Due to critical amino acid changes in the 72-89 sequence, the determinant of human (Hu) basic protein (BP) that induces experimental autoimmune encephalomyelitis (EAE) in Lewis rats most likely differs from rat and guinea pig BP. To discern encephalitogenic sequence(s), the immunodominant epitopes recognized by Hu-BP-specific T cell lines were identified using synthetic peptides that corresponded to the Hu-BP sequence. The Hu-BP-reactive T cell line contained two distinct specificities, one directed at the 87-99 (Hu) sequence restricted by I-E, and the second directed at the 55-74 (Hu) sequence restricted by I-A. T cells specific for the 87-99 determinant recognized both Hu- and Rt-BP, were highly encephalitogenic, and accounted for the experimental autoimmune encephalomyelitis-inducing activity of the Hu-BP line. T cells directed at the S55-74 (Hu) sequence did not recognize Rt-BP and were not encephalitogenic. The same TCR V genes (homologous to the mouse V alpha 2 and V beta 8 families) that we showed previously were utilized preferentially in response to the I-A restricted 72-89 encephalitogenic sequence were also present in T cell lines specific for both the S55-74 and S87-99 epitopes. These data indicate that encephalitogenic activity of BP in Lewis rats is related to discrete T cell epitopes that are present on or cross-react with rat-BP. Furthermore it would appear that genes in the TCR V alpha 2 and V beta 8 families are widely used in response to different BP epitopes restricted by either I-A or I-E molecules.

Authors+Show Affiliations

Neuroimmunology Research, VA Medical Center, Portland, OR 97201.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2479681

Citation

Vandenbark, A A., et al. "Determinants of Human Myelin Basic Protein That Induce Encephalitogenic T Cells in Lewis Rats." Journal of Immunology (Baltimore, Md. : 1950), vol. 143, no. 11, 1989, pp. 3512-6.
Vandenbark AA, Hashim GA, Celnik B, et al. Determinants of human myelin basic protein that induce encephalitogenic T cells in Lewis rats. J Immunol. 1989;143(11):3512-6.
Vandenbark, A. A., Hashim, G. A., Celnik, B., Galang, A., Li, X. B., Heber-Katz, E., & Offner, H. (1989). Determinants of human myelin basic protein that induce encephalitogenic T cells in Lewis rats. Journal of Immunology (Baltimore, Md. : 1950), 143(11), 3512-6.
Vandenbark AA, et al. Determinants of Human Myelin Basic Protein That Induce Encephalitogenic T Cells in Lewis Rats. J Immunol. 1989 Dec 1;143(11):3512-6. PubMed PMID: 2479681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determinants of human myelin basic protein that induce encephalitogenic T cells in Lewis rats. AU - Vandenbark,A A, AU - Hashim,G A, AU - Celnik,B, AU - Galang,A, AU - Li,X B, AU - Heber-Katz,E, AU - Offner,H, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 3512 EP - 6 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 143 IS - 11 N2 - Due to critical amino acid changes in the 72-89 sequence, the determinant of human (Hu) basic protein (BP) that induces experimental autoimmune encephalomyelitis (EAE) in Lewis rats most likely differs from rat and guinea pig BP. To discern encephalitogenic sequence(s), the immunodominant epitopes recognized by Hu-BP-specific T cell lines were identified using synthetic peptides that corresponded to the Hu-BP sequence. The Hu-BP-reactive T cell line contained two distinct specificities, one directed at the 87-99 (Hu) sequence restricted by I-E, and the second directed at the 55-74 (Hu) sequence restricted by I-A. T cells specific for the 87-99 determinant recognized both Hu- and Rt-BP, were highly encephalitogenic, and accounted for the experimental autoimmune encephalomyelitis-inducing activity of the Hu-BP line. T cells directed at the S55-74 (Hu) sequence did not recognize Rt-BP and were not encephalitogenic. The same TCR V genes (homologous to the mouse V alpha 2 and V beta 8 families) that we showed previously were utilized preferentially in response to the I-A restricted 72-89 encephalitogenic sequence were also present in T cell lines specific for both the S55-74 and S87-99 epitopes. These data indicate that encephalitogenic activity of BP in Lewis rats is related to discrete T cell epitopes that are present on or cross-react with rat-BP. Furthermore it would appear that genes in the TCR V alpha 2 and V beta 8 families are widely used in response to different BP epitopes restricted by either I-A or I-E molecules. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2479681/Determinants_of_human_myelin_basic_protein_that_induce_encephalitogenic_T_cells_in_Lewis_rats_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2479681 DB - PRIME DP - Unbound Medicine ER -