Tags

Type your tag names separated by a space and hit enter

Polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB gene are associated with carotid plaques but not with carotid intima-media thickness in patients with diabetes mellitus type 2.
Vasa 2014; 43(3):171-80VASA

Abstract

BACKGROUND

Apolipoprotein B is a key structural component of all the atherogenic lipoproteins (LDL, VLDL and IDL). Genetic variations of the ApoB gene may affect plasma ApoB and lipid levels, thus influencing atherogenesis. The present study was designed to investigate the association of polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB gene with plasma ApoB level, lipid levels and the different ultrasound phenotypes of carotid atherosclerosis in patients with diabetes mellitus type 2.

PATIENTS AND METHODS

595 patients with diabetes (399 on statin therapy and 196 without) and 200 healthy controls were enrolled in the study. The carotid intima-media thickness (CIMT) and plaque characteristics (presence and structure) were assessed ultrasonographically. Biochemical analyses were performed using standard biochemical methods. Both XbaI (rs693) and EcoRI (rs1042031) genotypes were determined by real-time PCR.

RESULTS

Genotype distributions and allele frequencies of the XbaI and EcoRI polymorphisms were not statistically significantly different between diabetic patients and controls. No statistically significant difference in lipid parameters, ApoA1, ApoB, hs-CRP and fibrinogen as well as CIMT was observed in diabetic patients regarding XbaI and EcoRI polymorphisms, even after adjustment for statin treatment. The risk of having plaques on carotid arteries was higher in homozygous carriers of the mutant X + allele (OR = 1.74, p = 0.03) and lower in diabetics carrying mutant E- alleles (OR = 0.48, p = 0.02). Neither XbaI nor EcoRI polymorphism was associated with CIMT or presence of unstable plaques in diabetic patients. Plasma ApoB level was not independently associated with any of the ultrasonographic parameters of carotid atherosclerosis.

CONCLUSIONS

Both XbaI and EcoRI polymorphisms were associated with presence of plaques on carotid arteries but not with CIMT or presence of unstable plaques. Plasma ApoB level was not independently associated with ultrasonographic phenotypes of carotid atherosclerosis in patients with diabetes mellitus.

Authors+Show Affiliations

Institute of Histology and Embryology, Medical Faculty, University of Ljubljana, Slovenia.General Hospital Rakičan, Murska Sobota, Slovenia.Department of Natural and Medical Sciences, Faculty of Health Sciences, University of Primorska, Slovenia.General Hospital Slovenj Gradec, Slovenia.General Hospital Slovenj Gradec, Slovenia.Institute of Histology and Embryology, Medical Faculty, University of Ljubljana, Slovenia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24797048

Citation

Nikolajevic Starcevic, Jovana, et al. "Polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB Gene Are Associated With Carotid Plaques but Not With Carotid Intima-media Thickness in Patients With Diabetes Mellitus Type 2." VASA. Zeitschrift Fur Gefasskrankheiten, vol. 43, no. 3, 2014, pp. 171-80.
Nikolajevic Starcevic J, Santl Letonja M, Praznikar ZJ, et al. Polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB gene are associated with carotid plaques but not with carotid intima-media thickness in patients with diabetes mellitus type 2. VASA. 2014;43(3):171-80.
Nikolajevic Starcevic, J., Santl Letonja, M., Praznikar, Z. J., Makuc, J., Vujkovac, A. C., & Petrovic, D. (2014). Polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB gene are associated with carotid plaques but not with carotid intima-media thickness in patients with diabetes mellitus type 2. VASA. Zeitschrift Fur Gefasskrankheiten, 43(3), pp. 171-80. doi:10.1024/0301-1526/a000346.
Nikolajevic Starcevic J, et al. Polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB Gene Are Associated With Carotid Plaques but Not With Carotid Intima-media Thickness in Patients With Diabetes Mellitus Type 2. VASA. 2014;43(3):171-80. PubMed PMID: 24797048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB gene are associated with carotid plaques but not with carotid intima-media thickness in patients with diabetes mellitus type 2. AU - Nikolajevic Starcevic,Jovana, AU - Santl Letonja,Marija, AU - Praznikar,Zala J, AU - Makuc,Jana, AU - Vujkovac,Andreja C, AU - Petrovic,Daniel, PY - 2014/5/7/entrez PY - 2014/5/7/pubmed PY - 2014/7/9/medline KW - Diabetes mellitus KW - apolipoprotein B KW - carotid atherosclerosis KW - polymorphism SP - 171 EP - 80 JF - VASA. Zeitschrift fur Gefasskrankheiten JO - VASA VL - 43 IS - 3 N2 - BACKGROUND: Apolipoprotein B is a key structural component of all the atherogenic lipoproteins (LDL, VLDL and IDL). Genetic variations of the ApoB gene may affect plasma ApoB and lipid levels, thus influencing atherogenesis. The present study was designed to investigate the association of polymorphisms XbaI (rs693) and EcoRI (rs1042031) of the ApoB gene with plasma ApoB level, lipid levels and the different ultrasound phenotypes of carotid atherosclerosis in patients with diabetes mellitus type 2. PATIENTS AND METHODS: 595 patients with diabetes (399 on statin therapy and 196 without) and 200 healthy controls were enrolled in the study. The carotid intima-media thickness (CIMT) and plaque characteristics (presence and structure) were assessed ultrasonographically. Biochemical analyses were performed using standard biochemical methods. Both XbaI (rs693) and EcoRI (rs1042031) genotypes were determined by real-time PCR. RESULTS: Genotype distributions and allele frequencies of the XbaI and EcoRI polymorphisms were not statistically significantly different between diabetic patients and controls. No statistically significant difference in lipid parameters, ApoA1, ApoB, hs-CRP and fibrinogen as well as CIMT was observed in diabetic patients regarding XbaI and EcoRI polymorphisms, even after adjustment for statin treatment. The risk of having plaques on carotid arteries was higher in homozygous carriers of the mutant X + allele (OR = 1.74, p = 0.03) and lower in diabetics carrying mutant E- alleles (OR = 0.48, p = 0.02). Neither XbaI nor EcoRI polymorphism was associated with CIMT or presence of unstable plaques in diabetic patients. Plasma ApoB level was not independently associated with any of the ultrasonographic parameters of carotid atherosclerosis. CONCLUSIONS: Both XbaI and EcoRI polymorphisms were associated with presence of plaques on carotid arteries but not with CIMT or presence of unstable plaques. Plasma ApoB level was not independently associated with ultrasonographic phenotypes of carotid atherosclerosis in patients with diabetes mellitus. SN - 0301-1526 UR - https://www.unboundmedicine.com/medline/citation/24797048/Polymorphisms_XbaI__rs693__and_EcoRI__rs1042031__of_the_ApoB_gene_are_associated_with_carotid_plaques_but_not_with_carotid_intima_media_thickness_in_patients_with_diabetes_mellitus_type_2_ L2 - http://econtent.hogrefe.com/doi/full/10.1024/0301-1526/a000346?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -