Tags

Type your tag names separated by a space and hit enter

OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.).
Plant J. 2014 Jul; 79(1):106-17.PJ

Abstract

Members of the ATP Binding Cassette B/Multidrug-Resistance/P-glyco-protein (ABCB/MDR/PGP) subfamily were shown to function primarily in Oryza sativa (rice) auxin transport; however, none of the rice ABCB transporters have been functionally characterized. Here, we describe that a knock-down of OsABCB14 confers decreased auxin concentrations and polar auxin transport rates, conferring insensitivity to 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-acetic acid (IAA). OsABCB14 displays enhanced specific auxin influx activity in yeast and protoplasts prepared from rice knock-down alleles. OsABCB14 is localized at the plasma membrane, pointing to an important directionality under physiological conditions. osabcb14 mutants were surprisingly found to be insensitive to iron deficiency treatment (-Fe). Their Fe concentration is higher and upregulation of Fe deficiency-responsive genes is lower in osabcb14 mutants than in wild-type rice (Nipponbare, NIP). Taken together, our results strongly support the role of OsABCB14 as an auxin influx transporter involved in Fe homeostasis. The functional characterization of OsABCB14 provides insights in monocot auxin transport and its relationship to Fe nutrition.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Xu Y
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China.
Zhang S
No affiliation info available
Guo H
No affiliation info available
Wang S
No affiliation info available
Xu L
No affiliation info available
Li C
No affiliation info available
Qian Q
No affiliation info available
Chen F
No affiliation info available
Geisler M
No affiliation info available
Qi Y
No affiliation info available
Jiang de A
No affiliation info available

MeSH

Biological TransportGene Expression Regulation, PlantGenes, ReporterHomeostasisIndoleacetic AcidsIronMembrane Transport ProteinsMutagenesis, InsertionalOryzaPlant Growth RegulatorsPlant ProteinsPlant RootsPlant ShootsPlants, Genetically ModifiedProtoplasts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24798203

Citation

Xu, Yanxia, et al. "OsABCB14 Functions in Auxin Transport and Iron Homeostasis in Rice (Oryza Sativa L.)." The Plant Journal : for Cell and Molecular Biology, vol. 79, no. 1, 2014, pp. 106-17.
Xu Y, Zhang S, Guo H, et al. OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.). Plant J. 2014;79(1):106-17.
Xu, Y., Zhang, S., Guo, H., Wang, S., Xu, L., Li, C., Qian, Q., Chen, F., Geisler, M., Qi, Y., & Jiang, d. e. . A. (2014). OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.). The Plant Journal : for Cell and Molecular Biology, 79(1), 106-17. https://doi.org/10.1111/tpj.12544
Xu Y, et al. OsABCB14 Functions in Auxin Transport and Iron Homeostasis in Rice (Oryza Sativa L.). Plant J. 2014;79(1):106-17. PubMed PMID: 24798203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.). AU - Xu,Yanxia, AU - Zhang,Saina, AU - Guo,Haipeng, AU - Wang,Suikang, AU - Xu,Ligen, AU - Li,Chuanyou, AU - Qian,Qian, AU - Chen,Fan, AU - Geisler,Markus, AU - Qi,Yanhua, AU - Jiang,De An, Y1 - 2014/06/13/ PY - 2013/11/18/received PY - 2014/03/15/revised PY - 2014/04/24/accepted PY - 2014/5/7/entrez PY - 2014/5/7/pubmed PY - 2015/2/25/medline KW - Oryza sativa L KW - OsABCB14 KW - iron homeostasis KW - polar auxin transport KW - rice SP - 106 EP - 17 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 79 IS - 1 N2 - Members of the ATP Binding Cassette B/Multidrug-Resistance/P-glyco-protein (ABCB/MDR/PGP) subfamily were shown to function primarily in Oryza sativa (rice) auxin transport; however, none of the rice ABCB transporters have been functionally characterized. Here, we describe that a knock-down of OsABCB14 confers decreased auxin concentrations and polar auxin transport rates, conferring insensitivity to 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-acetic acid (IAA). OsABCB14 displays enhanced specific auxin influx activity in yeast and protoplasts prepared from rice knock-down alleles. OsABCB14 is localized at the plasma membrane, pointing to an important directionality under physiological conditions. osabcb14 mutants were surprisingly found to be insensitive to iron deficiency treatment (-Fe). Their Fe concentration is higher and upregulation of Fe deficiency-responsive genes is lower in osabcb14 mutants than in wild-type rice (Nipponbare, NIP). Taken together, our results strongly support the role of OsABCB14 as an auxin influx transporter involved in Fe homeostasis. The functional characterization of OsABCB14 provides insights in monocot auxin transport and its relationship to Fe nutrition. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/24798203/OsABCB14_functions_in_auxin_transport_and_iron_homeostasis_in_rice__Oryza_sativa_L___ DB - PRIME DP - Unbound Medicine ER -