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Occurrence of Clostridium difficile PCR-ribotype 027 and it's closely related PCR-ribotype 176 in hospitals in Poland in 2008-2010.
Anaerobe. 2014 Aug; 28:13-7.A

Abstract

Since 2003, a rising incidence of Clostridium difficile infection (CDI) in North America and Europe has coincided with outbreaks of C. difficile PCR ribotype 027. This ribotype was not observed in Poland until 2008. In the period 2008-2010, outbreaks of antibiotic-associated diarrhoea occurred in three different hospitals in Poland. Of 30 C. difficile isolates available for microbiological characterisation, 17 (56%) were positive for binary toxin genes and belonged to PCR ribotype 027 (n = 7) and its closely related PCR ribotype 176 (n = 10). All 17 binary toxin-positive C. difficile strains demonstrated high-level resistance to fluoroquinolones (minimum inhibitory concentration (MIC) ≥ 32 mg/L), including ciprofloxacin, gatifloxacin, and moxifloxacin, as well as erythromycin and clindamycin (MIC ≥ 256 mg/L for both). Of 14 patients from whom clinical information was available, 50% had a severe form of CDI, defined by fever (>38.5 °C), decreased kidney function, and high leucocyte count. We conclude that outbreaks of CDI associated with hypervirulent strains belonging to PCR ribotypes 027 and 176 occurred in hospitals in Poland. Further studies evaluating the clinical impact of type 176 are urgently needed.

Authors+Show Affiliations

Department of Medical Microbiology, Medical University of Warsaw, 5 Chałubiński Street, 02-004 Warsaw, Poland.Department of Medical Microbiology, Medical University of Warsaw, 5 Chałubiński Street, 02-004 Warsaw, Poland.Department of Diagnostic Laboratory, Clinical Hospital of the Transfiguration, Poznań, Poland.Department of Diagnostic Laboratory, Clinical Hospital of the Transfiguration, Poznań, Poland.Diagnostic Laboratory, Provincial Hospital in Płock, Poland.Department of Internal Medicine and Cardiology, Infant Jesus Teaching Hospital in Warsaw, Medical University of Warsaw, Poland.Department of Internal Medicine and Cardiology, Infant Jesus Teaching Hospital in Warsaw, Medical University of Warsaw, Poland.Department of Medical Microbiology, Medical University of Warsaw, 5 Chałubiński Street, 02-004 Warsaw, Poland.Department of Medical Microbiology, Medical University of Warsaw, 5 Chałubiński Street, 02-004 Warsaw, Poland.Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands.Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands.bioMérieux R&D Microbiology, La Balme Les Grottes, France.Department of Medical Microbiology, Medical University of Warsaw, 5 Chałubiński Street, 02-004 Warsaw, Poland. Electronic address: hanna.pituch@wum.edu.pl.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24799338

Citation

Obuch-Woszczatyński, Piotr, et al. "Occurrence of Clostridium Difficile PCR-ribotype 027 and It's Closely Related PCR-ribotype 176 in Hospitals in Poland in 2008-2010." Anaerobe, vol. 28, 2014, pp. 13-7.
Obuch-Woszczatyński P, Lachowicz D, Schneider A, et al. Occurrence of Clostridium difficile PCR-ribotype 027 and it's closely related PCR-ribotype 176 in hospitals in Poland in 2008-2010. Anaerobe. 2014;28:13-7.
Obuch-Woszczatyński, P., Lachowicz, D., Schneider, A., Mól, A., Pawłowska, J., Ożdżeńska-Milke, E., Pruszczyk, P., Wultańska, D., Młynarczyk, G., Harmanus, C., Kuijper, E. J., van Belkum, A., & Pituch, H. (2014). Occurrence of Clostridium difficile PCR-ribotype 027 and it's closely related PCR-ribotype 176 in hospitals in Poland in 2008-2010. Anaerobe, 28, 13-7. https://doi.org/10.1016/j.anaerobe.2014.04.007
Obuch-Woszczatyński P, et al. Occurrence of Clostridium Difficile PCR-ribotype 027 and It's Closely Related PCR-ribotype 176 in Hospitals in Poland in 2008-2010. Anaerobe. 2014;28:13-7. PubMed PMID: 24799338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of Clostridium difficile PCR-ribotype 027 and it's closely related PCR-ribotype 176 in hospitals in Poland in 2008-2010. AU - Obuch-Woszczatyński,Piotr, AU - Lachowicz,Dominika, AU - Schneider,Anna, AU - Mól,Anna, AU - Pawłowska,Jolanta, AU - Ożdżeńska-Milke,Ewa, AU - Pruszczyk,Piotr, AU - Wultańska,Dorota, AU - Młynarczyk,Grażyna, AU - Harmanus,Celine, AU - Kuijper,Ed J, AU - van Belkum,Alex, AU - Pituch,Hanna, Y1 - 2014/05/02/ PY - 2013/12/14/received PY - 2014/04/03/revised PY - 2014/04/17/accepted PY - 2014/5/7/entrez PY - 2014/5/7/pubmed PY - 2015/4/2/medline KW - Clostridium difficile infection KW - Outbreak KW - PCR ribotype 027 KW - PCR ribotype 176 SP - 13 EP - 7 JF - Anaerobe JO - Anaerobe VL - 28 N2 - Since 2003, a rising incidence of Clostridium difficile infection (CDI) in North America and Europe has coincided with outbreaks of C. difficile PCR ribotype 027. This ribotype was not observed in Poland until 2008. In the period 2008-2010, outbreaks of antibiotic-associated diarrhoea occurred in three different hospitals in Poland. Of 30 C. difficile isolates available for microbiological characterisation, 17 (56%) were positive for binary toxin genes and belonged to PCR ribotype 027 (n = 7) and its closely related PCR ribotype 176 (n = 10). All 17 binary toxin-positive C. difficile strains demonstrated high-level resistance to fluoroquinolones (minimum inhibitory concentration (MIC) ≥ 32 mg/L), including ciprofloxacin, gatifloxacin, and moxifloxacin, as well as erythromycin and clindamycin (MIC ≥ 256 mg/L for both). Of 14 patients from whom clinical information was available, 50% had a severe form of CDI, defined by fever (>38.5 °C), decreased kidney function, and high leucocyte count. We conclude that outbreaks of CDI associated with hypervirulent strains belonging to PCR ribotypes 027 and 176 occurred in hospitals in Poland. Further studies evaluating the clinical impact of type 176 are urgently needed. SN - 1095-8274 UR - https://www.unboundmedicine.com/medline/citation/24799338/Occurrence_of_Clostridium_difficile_PCR_ribotype_027_and_it's_closely_related_PCR_ribotype_176_in_hospitals_in_Poland_in_2008_2010_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(14)00039-0 DB - PRIME DP - Unbound Medicine ER -