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Evaluation of three recombinant Leishmania infantum antigens in human and canine visceral leishmaniasis diagnosis.
Acta Trop. 2014 Sep; 137:25-30.AT

Abstract

Visceral leishmaniasis (VL) is a neglected disease and is fatal if untreated. Dogs serve as reservoirs for Leishmania infantum (syn. L. chagasi) due to their susceptibility to infection and high skin parasitism. Therefore, VL control in Brazil involves the elimination of seropositive dogs, among other actions. However, the most frequently used serological tests have limitations regarding sensitivity and specificity. In this study, we have selected three Leishmania antigens (C1, C8 and C9) and have produced them as recombinant proteins using pET-28a-TEV vector and Escherichia coli BL-21 as expression system. When tested in ELISA with human samples, the C9 antigen was the one showing the most promising results, with 68% sensitivity and 78% specificity. When testing canine samples, the C1, C8 and C9 antigens showed a sensitivity range from 70% to 80% and specificity range from 60% to 90%. The C1 antigen presented higher sensitivity (80%) and the C8 antigen presented higher specificity (90%). Due to it, we decided to mix and test C1 and C8 antigens together, resulting in the C18 antigen. The mix also yielded high percentages of detected symptomatic and asymptomatic dogs however it did not improve the performance of the diagnostic. Comparison of our tests with the tests recommended by the Brazilian Ministry of Health revealed that our antigens' sensitivities and the percentage of detected asymptomatic dogs were much higher. Our results suggest that the C1, C8, C18 and C9 recombinant proteins are good antigens to diagnose canine visceral leishmaniasis and could potentially be used in screening tests. To diagnose human visceral leishmaniasis, the C9 antigen presented reasonable results, but more optimization must be performed for this antigen to provide better performance.

Authors+Show Affiliations

Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Avenida Antônio Carlos, 6627, Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: helida@icb.ufmg.br.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24801885

Citation

Fonseca, Aliani Moura, et al. "Evaluation of Three Recombinant Leishmania Infantum Antigens in Human and Canine Visceral Leishmaniasis Diagnosis." Acta Tropica, vol. 137, 2014, pp. 25-30.
Fonseca AM, Faria AR, Rodrigues FT, et al. Evaluation of three recombinant Leishmania infantum antigens in human and canine visceral leishmaniasis diagnosis. Acta Trop. 2014;137:25-30.
Fonseca, A. M., Faria, A. R., Rodrigues, F. T., Nagem, R. A., Magalhães, R. D., Cunha, J. L., Bartholomeu, D. C., & de Andrade, H. M. (2014). Evaluation of three recombinant Leishmania infantum antigens in human and canine visceral leishmaniasis diagnosis. Acta Tropica, 137, 25-30. https://doi.org/10.1016/j.actatropica.2014.04.028
Fonseca AM, et al. Evaluation of Three Recombinant Leishmania Infantum Antigens in Human and Canine Visceral Leishmaniasis Diagnosis. Acta Trop. 2014;137:25-30. PubMed PMID: 24801885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of three recombinant Leishmania infantum antigens in human and canine visceral leishmaniasis diagnosis. AU - Fonseca,Aliani Moura, AU - Faria,Angélica Rosa, AU - Rodrigues,Fernandes Tenório Gomes, AU - Nagem,Ronaldo Alves Pinto, AU - Magalhães,Rubens Daniel Miserani, AU - Cunha,João Luís Reis, AU - Bartholomeu,Daniella Castanheira, AU - de Andrade,Hélida Monteiro, Y1 - 2014/05/04/ PY - 2013/10/22/received PY - 2014/04/16/revised PY - 2014/04/24/accepted PY - 2014/5/8/entrez PY - 2014/5/8/pubmed PY - 2015/3/3/medline KW - Antigens KW - Diagnosis KW - Leishmania infantum KW - Recombinant proteins KW - Visceral leishmaniasis SP - 25 EP - 30 JF - Acta tropica JO - Acta Trop VL - 137 N2 - Visceral leishmaniasis (VL) is a neglected disease and is fatal if untreated. Dogs serve as reservoirs for Leishmania infantum (syn. L. chagasi) due to their susceptibility to infection and high skin parasitism. Therefore, VL control in Brazil involves the elimination of seropositive dogs, among other actions. However, the most frequently used serological tests have limitations regarding sensitivity and specificity. In this study, we have selected three Leishmania antigens (C1, C8 and C9) and have produced them as recombinant proteins using pET-28a-TEV vector and Escherichia coli BL-21 as expression system. When tested in ELISA with human samples, the C9 antigen was the one showing the most promising results, with 68% sensitivity and 78% specificity. When testing canine samples, the C1, C8 and C9 antigens showed a sensitivity range from 70% to 80% and specificity range from 60% to 90%. The C1 antigen presented higher sensitivity (80%) and the C8 antigen presented higher specificity (90%). Due to it, we decided to mix and test C1 and C8 antigens together, resulting in the C18 antigen. The mix also yielded high percentages of detected symptomatic and asymptomatic dogs however it did not improve the performance of the diagnostic. Comparison of our tests with the tests recommended by the Brazilian Ministry of Health revealed that our antigens' sensitivities and the percentage of detected asymptomatic dogs were much higher. Our results suggest that the C1, C8, C18 and C9 recombinant proteins are good antigens to diagnose canine visceral leishmaniasis and could potentially be used in screening tests. To diagnose human visceral leishmaniasis, the C9 antigen presented reasonable results, but more optimization must be performed for this antigen to provide better performance. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/24801885/Evaluation_of_three_recombinant_Leishmania_infantum_antigens_in_human_and_canine_visceral_leishmaniasis_diagnosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(14)00151-X DB - PRIME DP - Unbound Medicine ER -