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Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blowfly.
Aust J Biol Sci. 1988; 41(2):269-78.AJ

Abstract

Isoelectric focusing was used to characterize proteolytic enzymes in homogenate and excretory-secretory preparations of the larvae of L. cuprina, the sheep blowfly. Zymogram overlays showed that the larvae produce a number of highly active proteases which have a wide range of isoelectric points and molecular weights. The alkaline and neutral pI proteases were inhibited by phenylmethyl-sulfonylfluoride, leupeptin and aprotinin; this indicated the presence of serine in the active site. Pepstatin and the metal chelating agent ethylenediaminetetraacetic acid had no effect on the activity of any of the proteases. Optimal pH for activity of the proteases was between 7 and 8. In addition, the proteases were found to be heat labile. Digestion of collagen fibrils confirmed the existence of collagenolytic activity in the excretory-secretory enzyme preparations. It is suggested that these enzymes may be involved in the nutrition of the larvae and in the pathogenesis of the lesion on the skin.

Authors+Show Affiliations

Bowles VM
School of Agriculture, La Trobe University, Bundoora, Vic.
Carnegie PR
No affiliation info available
Sandeman RM
No affiliation info available

MeSH

AnimalsAprotininCollagenDipteraGelatinHydrogen-Ion ConcentrationIsoelectric PointLarvaLeupeptinsMicrobial CollagenaseMolecular WeightPeptide HydrolasesPhenylmethylsulfonyl FluorideProtease Inhibitors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2480778

Citation

Bowles, V M., et al. "Characterization of Proteolytic and Collagenolytic Enzymes From the Larvae of Lucilia Cuprina, the Sheep Blowfly." Australian Journal of Biological Sciences, vol. 41, no. 2, 1988, pp. 269-78.
Bowles VM, Carnegie PR, Sandeman RM. Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blowfly. Aust J Biol Sci. 1988;41(2):269-78.
Bowles, V. M., Carnegie, P. R., & Sandeman, R. M. (1988). Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blowfly. Australian Journal of Biological Sciences, 41(2), 269-78.
Bowles VM, Carnegie PR, Sandeman RM. Characterization of Proteolytic and Collagenolytic Enzymes From the Larvae of Lucilia Cuprina, the Sheep Blowfly. Aust J Biol Sci. 1988;41(2):269-78. PubMed PMID: 2480778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blowfly. AU - Bowles,V M, AU - Carnegie,P R, AU - Sandeman,R M, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 269 EP - 78 JF - Australian journal of biological sciences JO - Aust J Biol Sci VL - 41 IS - 2 N2 - Isoelectric focusing was used to characterize proteolytic enzymes in homogenate and excretory-secretory preparations of the larvae of L. cuprina, the sheep blowfly. Zymogram overlays showed that the larvae produce a number of highly active proteases which have a wide range of isoelectric points and molecular weights. The alkaline and neutral pI proteases were inhibited by phenylmethyl-sulfonylfluoride, leupeptin and aprotinin; this indicated the presence of serine in the active site. Pepstatin and the metal chelating agent ethylenediaminetetraacetic acid had no effect on the activity of any of the proteases. Optimal pH for activity of the proteases was between 7 and 8. In addition, the proteases were found to be heat labile. Digestion of collagen fibrils confirmed the existence of collagenolytic activity in the excretory-secretory enzyme preparations. It is suggested that these enzymes may be involved in the nutrition of the larvae and in the pathogenesis of the lesion on the skin. SN - 0004-9417 UR - https://www.unboundmedicine.com/medline/citation/2480778/Characterization_of_proteolytic_and_collagenolytic_enzymes_from_the_larvae_of_Lucilia_cuprina_the_sheep_blowfly_ DB - PRIME DP - Unbound Medicine ER -