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Production of an endoinulinase from Aspergillus niger AUMC 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme.
J Microbiol. 2014 May; 52(5):389-98.JM

Abstract

Two different substrates, sunflower (Helianthus annuus L.) tubers and lettuce (Lactuca sativa) roots, were tested. Using a mixture of both wastes resulted in higher production of endoinulinase than either waste alone. Also, ten fungal species grown on these substrates as inexpensive, carbon sources were screened for the best production of endoinulinase activities. Of these, Aspergillus niger AUMC 9375 was the most productive, when grown on the mixture using a 6:1 w/w ratio of sun flower: lettuce, and yielded the highest levels of inulinase at 50% moisture, 30°C, pH 5.0, with seven days of incubation, and with yeast extract as the best nitrogen source. Inulinase was purified to homogeneity by ion-exchange chromatography and gel-filtration giving a 51.11 fold purification. The mixture of sunflower tubers and lettuce roots has potential to be an effective and economical substrate for inulinase production. Inulinase was successfully immobilized with an immobilization yield of 71.28%. After incubation for 2 h at 60°C, the free enzyme activity decreased markedly to 10%, whereas that of the immobilized form decreased only to 87%. A reusability test demonstrated the durability of the immobilized inulinase for 10 cycles and in addition, that it could be stored for 32 days at 4°C. These results indicate that this inulinase, in the immobilized form, is a potential candidate for large-scale production of high purity fructose syrups.

Authors+Show Affiliations

Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo, 11757, Egypt, Manal_Maher2@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24810318

Citation

Housseiny, Manal M.. "Production of an Endoinulinase From Aspergillus Niger AUMC 9375, By Solid State Fermentation of Agricultural Wastes, With Purification and Characterization of the Free and Immobilized Enzyme." Journal of Microbiology (Seoul, Korea), vol. 52, no. 5, 2014, pp. 389-98.
Housseiny MM. Production of an endoinulinase from Aspergillus niger AUMC 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme. J Microbiol. 2014;52(5):389-98.
Housseiny, M. M. (2014). Production of an endoinulinase from Aspergillus niger AUMC 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme. Journal of Microbiology (Seoul, Korea), 52(5), 389-98. https://doi.org/10.1007/s12275-014-3561-y
Housseiny MM. Production of an Endoinulinase From Aspergillus Niger AUMC 9375, By Solid State Fermentation of Agricultural Wastes, With Purification and Characterization of the Free and Immobilized Enzyme. J Microbiol. 2014;52(5):389-98. PubMed PMID: 24810318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of an endoinulinase from Aspergillus niger AUMC 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme. A1 - Housseiny,Manal M, Y1 - 2014/05/09/ PY - 2013/10/25/received PY - 2013/12/02/accepted PY - 2013/11/29/revised PY - 2014/5/10/entrez PY - 2014/5/9/pubmed PY - 2014/12/17/medline SP - 389 EP - 98 JF - Journal of microbiology (Seoul, Korea) JO - J. Microbiol. VL - 52 IS - 5 N2 - Two different substrates, sunflower (Helianthus annuus L.) tubers and lettuce (Lactuca sativa) roots, were tested. Using a mixture of both wastes resulted in higher production of endoinulinase than either waste alone. Also, ten fungal species grown on these substrates as inexpensive, carbon sources were screened for the best production of endoinulinase activities. Of these, Aspergillus niger AUMC 9375 was the most productive, when grown on the mixture using a 6:1 w/w ratio of sun flower: lettuce, and yielded the highest levels of inulinase at 50% moisture, 30°C, pH 5.0, with seven days of incubation, and with yeast extract as the best nitrogen source. Inulinase was purified to homogeneity by ion-exchange chromatography and gel-filtration giving a 51.11 fold purification. The mixture of sunflower tubers and lettuce roots has potential to be an effective and economical substrate for inulinase production. Inulinase was successfully immobilized with an immobilization yield of 71.28%. After incubation for 2 h at 60°C, the free enzyme activity decreased markedly to 10%, whereas that of the immobilized form decreased only to 87%. A reusability test demonstrated the durability of the immobilized inulinase for 10 cycles and in addition, that it could be stored for 32 days at 4°C. These results indicate that this inulinase, in the immobilized form, is a potential candidate for large-scale production of high purity fructose syrups. SN - 1976-3794 UR - https://www.unboundmedicine.com/medline/citation/24810318/Production_of_an_endoinulinase_from_Aspergillus_niger_AUMC_9375_by_solid_state_fermentation_of_agricultural_wastes_with_purification_and_characterization_of_the_free_and_immobilized_enzyme_ L2 - https://dx.doi.org/10.1007/s12275-014-3561-y DB - PRIME DP - Unbound Medicine ER -