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Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function.

Abstract

AIM

Candida albicans is a pathogenic yeast, which forms a range of polarized and expanded cell shapes. We aimed to determine the correlation between honey extract (HFE) activity and changes in C. albicans cell cycle, morphology and subcellular organelles.

MATERIALS & METHODS

HFE anticandidal properties were investigated using flow cytometry and scanning electron microscopy.

RESULTS

Flow cytometry and scanning electron microscopy analyses indicated that HFE may inhibit the growth of the three phenotypes displayed by C. albicans and reduce infection by affecting membrane integrity. HFE affects hyphal transition by reducing the G0/G1 phase and increasing the G2/M phase. Conversely, yeast and pseudohyphae do not appear to be affected. Modifications of vacuolization and mitochondrial activity, during yeast-hypha transition establish the involvement of vacuole and mitochondria.

CONCLUSION

HFE improved mitochondrial functionality and reduced the vacuolization, modifying the branching process associated with virulence. It is hypothesized that HFE induces changes in cell cycle progress, membrane integrity, mitochondrial function and biogenesis.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Earth, Life & Environmental Sciences, Urbino, Italy.

    , , , , , ,

    Source

    Future microbiology 9:4 2014 pg 445-56

    MeSH

    Antifungal Agents
    Candida albicans
    Cell Cycle
    DNA, Fungal
    Flavonoids
    Flow Cytometry
    Honey
    Hyphae
    Microscopy, Electron, Scanning
    Mitochondria

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    24810344