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Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis.
Tumour Biol 2014; 35(8):7743-54TB

Abstract

LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan-Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention.

Authors+Show Affiliations

Department of Surgical Oncology, Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24810925

Citation

Xie, Hai-Wei, et al. "Long Noncoding RNA SPRY4-IT1 Is Upregulated in Esophageal Squamous Cell Carcinoma and Associated With Poor Prognosis." Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, vol. 35, no. 8, 2014, pp. 7743-54.
Xie HW, Wu QQ, Zhu B, et al. Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis. Tumour Biol. 2014;35(8):7743-54.
Xie, H. W., Wu, Q. Q., Zhu, B., Chen, F. J., Ji, L., Li, S. Q., ... Cao, X. F. (2014). Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis. Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, 35(8), pp. 7743-54. doi:10.1007/s13277-014-2013-y.
Xie HW, et al. Long Noncoding RNA SPRY4-IT1 Is Upregulated in Esophageal Squamous Cell Carcinoma and Associated With Poor Prognosis. Tumour Biol. 2014;35(8):7743-54. PubMed PMID: 24810925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis. AU - Xie,Hai-Wei, AU - Wu,Qing-Quan, AU - Zhu,Bin, AU - Chen,Fang-Jun, AU - Ji,Lv, AU - Li,Su-Qing, AU - Wang,Chun-Mei, AU - Tong,Yu-Suo, AU - Tuo,Lei, AU - Wu,Ming, AU - Liu,Zhi-Hua, AU - Lv,Jin, AU - Shi,Wei-Hong, AU - Cao,Xiu-Feng, Y1 - 2014/05/09/ PY - 2014/01/27/received PY - 2014/04/23/accepted PY - 2014/5/10/entrez PY - 2014/5/9/pubmed PY - 2015/1/15/medline SP - 7743 EP - 54 JF - Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine JO - Tumour Biol. VL - 35 IS - 8 N2 - LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan-Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention. SN - 1423-0380 UR - https://www.unboundmedicine.com/medline/citation/24810925/Long_noncoding_RNA_SPRY4_IT1_is_upregulated_in_esophageal_squamous_cell_carcinoma_and_associated_with_poor_prognosis_ L2 - https://link.springer.com/article/10.1007/s13277-014-2013-y DB - PRIME DP - Unbound Medicine ER -