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Hfq protein deficiency in Escherichia coli affects ColE1-like but not λ plasmid DNA replication.
Plasmid. 2014 May; 73:10-5.P

Abstract

Hfq is a nucleic acid-binding protein involved in controlling several aspects of RNA metabolism. It achieves this regulatory function by modulating the translational activity and stability of different mRNAs, generally via interactions with stress-related small regulatory sRNAs. However, besides its role in the coordination of translation of bacterial mRNA, Hfq is also a nucleoid-associated DNA-binding protein. Motivated by the above property of Hfq, we investigated if hfq gene mutation has implications for the regulation of DNA replication. Efficiency of ColE1-like (pMB1- and p15A replicons) and bacteriophage λ-derived plasmids' replication has been investigated in wild-type strain and otherwise isogenic hfq mutant of Escherichia coli. Significant differences in plasmid amount and kinetics of plasmid DNA synthesis were observed between the two tested bacterial hosts for ColE1-like replicons, but not for λ plasmid. Furthermore, ColE1-like plasmids replicated more efficiently in wild-type cells than in the hfq mutant in the early exponential phase of growth, but less efficiently in late exponential and early stationary phases. Hfq levels in the wild-type host, estimated by Western-blotting, were increased at the latter phases relative to the former one. Moreover, effects of the hfq mutation on ColE1-like plasmid replication were impaired in the absence of the rom gene, coding for a protein enhancing RNA I-RNA II interactions during the control of the replication initiation. These results are discussed in the light of a potential mechanism by which Hfq protein may influence replication of some, but not all, replicons in E. coli.

Authors+Show Affiliations

Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland. Electronic address: grzegorz.cech@biol.ug.edu.pl.Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland.Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland.Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland.Univ Paris Diderot-Paris 7, Sorbone Paris Cité, 75013 Paris, France; Laboratoire Léon Brillouin UMR12 CEA CNRS, CEA-Saclay, 91191 Gif-sur-Yvette Cedex, France.Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdańsk, Poland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24811974

Citation

Cech, Grzegorz M., et al. "Hfq Protein Deficiency in Escherichia Coli Affects ColE1-like but Not Λ Plasmid DNA Replication." Plasmid, vol. 73, 2014, pp. 10-5.
Cech GM, Pakuła B, Kamrowska D, et al. Hfq protein deficiency in Escherichia coli affects ColE1-like but not λ plasmid DNA replication. Plasmid. 2014;73:10-5.
Cech, G. M., Pakuła, B., Kamrowska, D., Węgrzyn, G., Arluison, V., & Szalewska-Pałasz, A. (2014). Hfq protein deficiency in Escherichia coli affects ColE1-like but not λ plasmid DNA replication. Plasmid, 73, 10-5. https://doi.org/10.1016/j.plasmid.2014.04.005
Cech GM, et al. Hfq Protein Deficiency in Escherichia Coli Affects ColE1-like but Not Λ Plasmid DNA Replication. Plasmid. 2014;73:10-5. PubMed PMID: 24811974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hfq protein deficiency in Escherichia coli affects ColE1-like but not λ plasmid DNA replication. AU - Cech,Grzegorz M, AU - Pakuła,Bartosz, AU - Kamrowska,Dominika, AU - Węgrzyn,Grzegorz, AU - Arluison,Véronique, AU - Szalewska-Pałasz,Agnieszka, Y1 - 2014/05/05/ PY - 2013/12/09/received PY - 2014/04/21/revised PY - 2014/04/23/accepted PY - 2014/5/10/entrez PY - 2014/5/9/pubmed PY - 2015/1/7/medline KW - ColE1 and lambda plasmids KW - DNA replication KW - Hfq Sm-like protein KW - Nucleoid KW - Small noncoding RNA SP - 10 EP - 5 JF - Plasmid JO - Plasmid VL - 73 N2 - Hfq is a nucleic acid-binding protein involved in controlling several aspects of RNA metabolism. It achieves this regulatory function by modulating the translational activity and stability of different mRNAs, generally via interactions with stress-related small regulatory sRNAs. However, besides its role in the coordination of translation of bacterial mRNA, Hfq is also a nucleoid-associated DNA-binding protein. Motivated by the above property of Hfq, we investigated if hfq gene mutation has implications for the regulation of DNA replication. Efficiency of ColE1-like (pMB1- and p15A replicons) and bacteriophage λ-derived plasmids' replication has been investigated in wild-type strain and otherwise isogenic hfq mutant of Escherichia coli. Significant differences in plasmid amount and kinetics of plasmid DNA synthesis were observed between the two tested bacterial hosts for ColE1-like replicons, but not for λ plasmid. Furthermore, ColE1-like plasmids replicated more efficiently in wild-type cells than in the hfq mutant in the early exponential phase of growth, but less efficiently in late exponential and early stationary phases. Hfq levels in the wild-type host, estimated by Western-blotting, were increased at the latter phases relative to the former one. Moreover, effects of the hfq mutation on ColE1-like plasmid replication were impaired in the absence of the rom gene, coding for a protein enhancing RNA I-RNA II interactions during the control of the replication initiation. These results are discussed in the light of a potential mechanism by which Hfq protein may influence replication of some, but not all, replicons in E. coli. SN - 1095-9890 UR - https://www.unboundmedicine.com/medline/citation/24811974/Hfq_protein_deficiency_in_Escherichia_coli_affects_ColE1_like_but_not_λ_plasmid_DNA_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(14)00018-3 DB - PRIME DP - Unbound Medicine ER -