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Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease.
Scand J Immunol. 1989 Dec; 30(6):665-72.SJ

Abstract

Expression of the gamma/delta T-cell receptor (TcR) for antigen on CD3+ intraepithelial lymphocytes (IEL) was studied in situ by two-colour immunofluorescence on jejunal tissue sections from 24 patients with coeliac disease and 17 controls. The proportion of intraepithelial TcR gamma/delta+ cells was significantly increased (P less than 0.002) in untreated (median 20%, range 11-53%) as well as in treated (gluten-free diet) coeliac disease (median 23%, range 16-55%) compared with controls (median 2%, range 0-39%). Although TcR alpha/beta+ IEL dominated both in controls and coeliac disease, T cells expressing the TcR gamma/delta were preferentially located within the epithelium rather than in the lamina propria. Paired staining for TcR gamma/delta and CD8 revealed that most (approximately 90%) intraepithelial TcR gamma/delta+ lymphocytes in coeliac disease were CD8-. A remarkably large fraction (median 67%, range 58-94%) of intraepithelial TcR gamma/delta+ cells expressed the V delta 1/J delta 1-encoded epitope revealed by monoclonal antibody delta TCS1. Our results suggested that increase of the intraepithelial TcR gamma/delta+ CD8- subset of T cells is particularly related to coeliac disease.

Authors+Show Affiliations

Institute of Pathology, University of Oslo, Rikshospitalet, Norway.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2481336

Citation

Halstensen, T S., et al. "Intraepithelial T Cells of the TcR Gamma/delta+ CD8- and V Delta 1/J Delta 1+ Phenotypes Are Increased in Coeliac Disease." Scandinavian Journal of Immunology, vol. 30, no. 6, 1989, pp. 665-72.
Halstensen TS, Scott H, Brandtzaeg P. Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease. Scand J Immunol. 1989;30(6):665-72.
Halstensen, T. S., Scott, H., & Brandtzaeg, P. (1989). Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease. Scandinavian Journal of Immunology, 30(6), 665-72.
Halstensen TS, Scott H, Brandtzaeg P. Intraepithelial T Cells of the TcR Gamma/delta+ CD8- and V Delta 1/J Delta 1+ Phenotypes Are Increased in Coeliac Disease. Scand J Immunol. 1989;30(6):665-72. PubMed PMID: 2481336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease. AU - Halstensen,T S, AU - Scott,H, AU - Brandtzaeg,P, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 665 EP - 72 JF - Scandinavian journal of immunology JO - Scand J Immunol VL - 30 IS - 6 N2 - Expression of the gamma/delta T-cell receptor (TcR) for antigen on CD3+ intraepithelial lymphocytes (IEL) was studied in situ by two-colour immunofluorescence on jejunal tissue sections from 24 patients with coeliac disease and 17 controls. The proportion of intraepithelial TcR gamma/delta+ cells was significantly increased (P less than 0.002) in untreated (median 20%, range 11-53%) as well as in treated (gluten-free diet) coeliac disease (median 23%, range 16-55%) compared with controls (median 2%, range 0-39%). Although TcR alpha/beta+ IEL dominated both in controls and coeliac disease, T cells expressing the TcR gamma/delta were preferentially located within the epithelium rather than in the lamina propria. Paired staining for TcR gamma/delta and CD8 revealed that most (approximately 90%) intraepithelial TcR gamma/delta+ lymphocytes in coeliac disease were CD8-. A remarkably large fraction (median 67%, range 58-94%) of intraepithelial TcR gamma/delta+ cells expressed the V delta 1/J delta 1-encoded epitope revealed by monoclonal antibody delta TCS1. Our results suggested that increase of the intraepithelial TcR gamma/delta+ CD8- subset of T cells is particularly related to coeliac disease. SN - 0300-9475 UR - https://www.unboundmedicine.com/medline/citation/2481336/Intraepithelial_T_cells_of_the_TcR_gamma/delta+_CD8__and_V_delta_1/J_delta_1+_phenotypes_are_increased_in_coeliac_disease_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0300-9475&date=1989&volume=30&issue=6&spage=665 DB - PRIME DP - Unbound Medicine ER -