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Effect of acetaminophen exposure in Oncorhynchus mykiss gills and liver: detoxification mechanisms, oxidative defence system and peroxidative damage.
Environ Toxicol Pharmacol. 2014 May; 37(3):1221-8.ET

Abstract

The increasing presence of pharmaceutical drugs in nature is cause of concern due to the occurrence of oxidative stress in non-target species. Acetaminophen is widely used in human medicine as an analgesic and antipyretic drug, and it is one of the most sold non-prescription drugs. The present study aimed to assess the toxic effects of acetaminophen (APAP) in Oncorhynchus mykiss following acute and chronic exposures in realistic levels. In order to evaluate the APAP effects in the rainbow trout, gills and liver were analyzed with biochemical biomarkers, such as catalase (CAT), total and selenium-dependent glutathione peroxidase (GPx), glutathione reductase (GRed) and glutathione-S-transferases (GSTs) activity and also lipid peroxidation levels (TBARS). The results obtained in all tests indicate that a significant response of oxidative stress was established, along with the increase of APAP concentrations. The establishment of an oxidative stress scenario occurred with the involvement of all tested biomarkers, sustaining a generalized set of pro-oxidative effects elicited by APAP. Additionally, the occurrence of oxidative damage strongly suggests the impairment of the antioxidant defense mechanism of O. mykiss. It is important to note that the occurrence of oxidative deleterious effects and peroxidative damages occurred for concentrations similar to those already reported for several freshwater ecosystems. The importance of these assumptions is further discussed under the scope of ecological relevance of the assessment of effects caused by pharmaceuticals in non-target organisms.

Authors+Show Affiliations

Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Rua dos Bragas 289, 450-123 Porto, Portugal; Faculdade de Ciências da Universidade do Porto (FCUP), Departamento de Biologia, Rua do Campo Alegre S/N, 4169-007 Porto, Portugal.Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Rua dos Bragas 289, 450-123 Porto, Portugal; Faculdade de Ciências da Saúde da Universidade Fernando Pessoa (FCS-UFP), Rua Carlos da Maia 296, 4200-150, Porto, Portugal.Faculdade de Ciências da Universidade do Porto (FCUP), Departamento de Biologia, Rua do Campo Alegre S/N, 4169-007 Porto, Portugal; Centro de Estudos do Ambiente e do Mar (CESAM), Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.Centro de Estudos do Ambiente e do Mar (CESAM), Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.Centro de Estudos do Ambiente e do Mar (CESAM), Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address: nunes.b@ua.pt.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24816177

Citation

Ramos, A S., et al. "Effect of Acetaminophen Exposure in Oncorhynchus Mykiss Gills and Liver: Detoxification Mechanisms, Oxidative Defence System and Peroxidative Damage." Environmental Toxicology and Pharmacology, vol. 37, no. 3, 2014, pp. 1221-8.
Ramos AS, Correia AT, Antunes SC, et al. Effect of acetaminophen exposure in Oncorhynchus mykiss gills and liver: detoxification mechanisms, oxidative defence system and peroxidative damage. Environ Toxicol Pharmacol. 2014;37(3):1221-8.
Ramos, A. S., Correia, A. T., Antunes, S. C., Gonçalves, F., & Nunes, B. (2014). Effect of acetaminophen exposure in Oncorhynchus mykiss gills and liver: detoxification mechanisms, oxidative defence system and peroxidative damage. Environmental Toxicology and Pharmacology, 37(3), 1221-8. https://doi.org/10.1016/j.etap.2014.04.005
Ramos AS, et al. Effect of Acetaminophen Exposure in Oncorhynchus Mykiss Gills and Liver: Detoxification Mechanisms, Oxidative Defence System and Peroxidative Damage. Environ Toxicol Pharmacol. 2014;37(3):1221-8. PubMed PMID: 24816177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of acetaminophen exposure in Oncorhynchus mykiss gills and liver: detoxification mechanisms, oxidative defence system and peroxidative damage. AU - Ramos,A S, AU - Correia,A T, AU - Antunes,S C, AU - Gonçalves,F, AU - Nunes,B, Y1 - 2014/04/13/ PY - 2013/12/13/received PY - 2014/04/01/revised PY - 2014/04/02/accepted PY - 2014/5/13/entrez PY - 2014/5/13/pubmed PY - 2015/1/30/medline KW - Biomarkers KW - Oxidative stress KW - Paracetamol KW - Pharmaceuticals KW - Rainbow trout SP - 1221 EP - 8 JF - Environmental toxicology and pharmacology JO - Environ Toxicol Pharmacol VL - 37 IS - 3 N2 - The increasing presence of pharmaceutical drugs in nature is cause of concern due to the occurrence of oxidative stress in non-target species. Acetaminophen is widely used in human medicine as an analgesic and antipyretic drug, and it is one of the most sold non-prescription drugs. The present study aimed to assess the toxic effects of acetaminophen (APAP) in Oncorhynchus mykiss following acute and chronic exposures in realistic levels. In order to evaluate the APAP effects in the rainbow trout, gills and liver were analyzed with biochemical biomarkers, such as catalase (CAT), total and selenium-dependent glutathione peroxidase (GPx), glutathione reductase (GRed) and glutathione-S-transferases (GSTs) activity and also lipid peroxidation levels (TBARS). The results obtained in all tests indicate that a significant response of oxidative stress was established, along with the increase of APAP concentrations. The establishment of an oxidative stress scenario occurred with the involvement of all tested biomarkers, sustaining a generalized set of pro-oxidative effects elicited by APAP. Additionally, the occurrence of oxidative damage strongly suggests the impairment of the antioxidant defense mechanism of O. mykiss. It is important to note that the occurrence of oxidative deleterious effects and peroxidative damages occurred for concentrations similar to those already reported for several freshwater ecosystems. The importance of these assumptions is further discussed under the scope of ecological relevance of the assessment of effects caused by pharmaceuticals in non-target organisms. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/24816177/Effect_of_acetaminophen_exposure_in_Oncorhynchus_mykiss_gills_and_liver:_detoxification_mechanisms_oxidative_defence_system_and_peroxidative_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(14)00089-1 DB - PRIME DP - Unbound Medicine ER -