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Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil.
Antonie Van Leeuwenhoek. 2014 Aug; 106(2):227-33.AV

Abstract

Staphylococci are one of the most prevalent microorganisms in bovine mastitis. Staphylococcus spp. are widespread in the environment, and can infect animals and humans as opportunistic pathogens. The objective of this study was to determine the frequency of methicillin-resistance (MR) among coagulase-negative staphylococci (CoNS) previously obtained from milk of mastitic cows in Brazil and to characterize the antimicrobial resistance phenotype/genotype and the SCCmec type of MRCoNS isolates. Identification of MRCoNS was based on both biochemical and molecular methods. Susceptibility testing for eleven antimicrobials was performed by disk-diffusion agar. Antimicrobial resistance genes and SCCmec were investigated by specific PCRs. Twenty-six MRCoNS were detected (20 % of total CoNS), obtained from 24 animals, and were identified as follows: S. epidermidis (7 isolates), S. chromogenes (7), S. warneri (6), S. hyicus (5) and S. simulans (1). All MRCoNS isolates carried mecA while the mecC gene was not detected in any CoNS. The SCCmec IVa was demonstrated in nine MRCoNS, while the remaining 17 isolates harbored non-typeable SCCmec cassettes. In addition to oxacillin and cefoxitin resistance, MRCoNS showed resistance to tetracycline (n = 7), streptomycin (n = 6), tobramycin (n = 6), and gentamicin (n = 4), and harbored the genes tet(K) (n = 7), str (n = 3), ant(4') (n = 6) and aac(6')-aph(2″) (n = 4), respectively. In addition, seven strains showed intermediate resistance to clindamycin and two to streptomycin, of which two harboured the lnu(B) and lsa(E) genes and two the aad(E) gene, respectively. One isolate presented intermediate erythromycin and clindamycin resistance and harbored an erm(C) gene with an uncommon 89-bp deletion rendering a premature stop codon. MRCoNS can be implicated in mastitis of cows and they constitute a reservoir of resistance genes that can be transferred to other pathogenic bacteria.

Authors+Show Affiliations

Department of Microbiology and Immunology, UNESP, Botucatu, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24817534

Citation

Silva, Nathalia C C., et al. "Characterization of Methicillin-resistant Coagulase-negative Staphylococci in Milk From Cows With Mastitis in Brazil." Antonie Van Leeuwenhoek, vol. 106, no. 2, 2014, pp. 227-33.
Silva NC, Guimarães FF, de P Manzi M, et al. Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil. Antonie Van Leeuwenhoek. 2014;106(2):227-33.
Silva, N. C., Guimarães, F. F., de P Manzi, M., Gómez-Sanz, E., Gómez, P., Araújo-Júnior, J. P., Langoni, H., Rall, V. L., & Torres, C. (2014). Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil. Antonie Van Leeuwenhoek, 106(2), 227-33. https://doi.org/10.1007/s10482-014-0185-5
Silva NC, et al. Characterization of Methicillin-resistant Coagulase-negative Staphylococci in Milk From Cows With Mastitis in Brazil. Antonie Van Leeuwenhoek. 2014;106(2):227-33. PubMed PMID: 24817534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil. AU - Silva,Nathalia C C, AU - Guimarães,Felipe F, AU - de P Manzi,Marcela, AU - Gómez-Sanz,Elena, AU - Gómez,Paula, AU - Araújo-Júnior,Joao P, AU - Langoni,Helio, AU - Rall,Vera L M, AU - Torres,Carmen, Y1 - 2014/05/10/ PY - 2014/01/19/received PY - 2014/04/22/accepted PY - 2014/5/13/entrez PY - 2014/5/13/pubmed PY - 2015/8/20/medline SP - 227 EP - 33 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 106 IS - 2 N2 - Staphylococci are one of the most prevalent microorganisms in bovine mastitis. Staphylococcus spp. are widespread in the environment, and can infect animals and humans as opportunistic pathogens. The objective of this study was to determine the frequency of methicillin-resistance (MR) among coagulase-negative staphylococci (CoNS) previously obtained from milk of mastitic cows in Brazil and to characterize the antimicrobial resistance phenotype/genotype and the SCCmec type of MRCoNS isolates. Identification of MRCoNS was based on both biochemical and molecular methods. Susceptibility testing for eleven antimicrobials was performed by disk-diffusion agar. Antimicrobial resistance genes and SCCmec were investigated by specific PCRs. Twenty-six MRCoNS were detected (20 % of total CoNS), obtained from 24 animals, and were identified as follows: S. epidermidis (7 isolates), S. chromogenes (7), S. warneri (6), S. hyicus (5) and S. simulans (1). All MRCoNS isolates carried mecA while the mecC gene was not detected in any CoNS. The SCCmec IVa was demonstrated in nine MRCoNS, while the remaining 17 isolates harbored non-typeable SCCmec cassettes. In addition to oxacillin and cefoxitin resistance, MRCoNS showed resistance to tetracycline (n = 7), streptomycin (n = 6), tobramycin (n = 6), and gentamicin (n = 4), and harbored the genes tet(K) (n = 7), str (n = 3), ant(4') (n = 6) and aac(6')-aph(2″) (n = 4), respectively. In addition, seven strains showed intermediate resistance to clindamycin and two to streptomycin, of which two harboured the lnu(B) and lsa(E) genes and two the aad(E) gene, respectively. One isolate presented intermediate erythromycin and clindamycin resistance and harbored an erm(C) gene with an uncommon 89-bp deletion rendering a premature stop codon. MRCoNS can be implicated in mastitis of cows and they constitute a reservoir of resistance genes that can be transferred to other pathogenic bacteria. SN - 1572-9699 UR - https://www.unboundmedicine.com/medline/citation/24817534/Characterization_of_methicillin_resistant_coagulase_negative_staphylococci_in_milk_from_cows_with_mastitis_in_Brazil_ L2 - https://doi.org/10.1007/s10482-014-0185-5 DB - PRIME DP - Unbound Medicine ER -