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Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jun 01; 960:214-21.JC

Abstract

Peptide drugs play a critical role in therapeutic treatment. However, as the complexity of plasma, determination of peptide drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a daunting task. To solve this problem, hydrophilic interaction liquid chromatography-solid phase extraction (HILIC-SPE) directly combined with protein precipitation (PPT) was developed for the selective extraction of triptorelin from plasma. The extracts were analyzed by reversed-phase liquid chromatography (RPLC). Proteins, phospholipids and highly polar interferences could be removed from plasma by the efficient combination of PPT, HILIC-SPE and RPLC-MS/MS. This method was evaluated by matrix effect, recovery and process efficiency at different concentration levels (50, 500 and 5,000 ng/mL) of triptorelin. Furthermore, the performance of HILIC-SPE was compared with that of reversed-phase C18 SPE and hydrophilic lipophilic balance (Oasis HLB) SPE. Among them, HILIC-SPE provided the minimum matrix effect (ranging from 96.02% to 103.41%), the maximum recovery (ranging from 80.68% to 90.54%) and the satisfactory process efficiency (ranging from 82.83% to 92.95%). The validated method was successfully applied to determine triptorelin in rat plasma.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Department of Radiation Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: zhangxiuli@dicp.ac.cn.

Department of Radiation Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China. Electronic address: zoulijuan1963@sina.com.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

MeSH

AnimalsChemical PrecipitationChromatography, LiquidDrug StabilityHydrophobic and Hydrophilic InteractionsPhospholipidsProteinsRatsSensitivity and SpecificitySolid Phase ExtractionTandem Mass SpectrometryTriptorelin Pamoate

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24820974

Citation

Wang, Jixia, et al. "Hydrophilic Interaction Liquid Chromatography-solid Phase Extraction Directly Combined With Protein Precipitation for the Determination of Triptorelin in Plasma." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 960, 2014, pp. 214-21.

Wang J, Kong S, Yan J, et al. Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2014;960:214-21.

Wang, J., Kong, S., Yan, J., Jin, G., Guo, Z., Shen, A., Xu, J., Zhang, X., Zou, L., & Liang, X. (2014). Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 960, 214-21. https://doi.org/10.1016/j.jchromb.2014.04.036

Wang J, et al. Hydrophilic Interaction Liquid Chromatography-solid Phase Extraction Directly Combined With Protein Precipitation for the Determination of Triptorelin in Plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jun 1;960:214-21. PubMed PMID: 24820974.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma. AU - Wang,Jixia, AU - Kong,Song, AU - Yan,Jingyu, AU - Jin,Gaowa, AU - Guo,Zhimou, AU - Shen,Aijin, AU - Xu,Junyan, AU - Zhang,Xiuli, AU - Zou,Lijuan, AU - Liang,Xinmiao, Y1 - 2014/04/28/ PY - 2014/01/23/received PY - 2014/04/15/revised PY - 2014/04/19/accepted PY - 2014/5/14/entrez PY - 2014/5/14/pubmed PY - 2015/1/13/medline KW - HILIC-SPE KW - PPT KW - Plasma KW - RPLC–MS/MS KW - Triptorelin SP - 214 EP - 21 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 960 N2 - Peptide drugs play a critical role in therapeutic treatment. However, as the complexity of plasma, determination of peptide drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a daunting task. To solve this problem, hydrophilic interaction liquid chromatography-solid phase extraction (HILIC-SPE) directly combined with protein precipitation (PPT) was developed for the selective extraction of triptorelin from plasma. The extracts were analyzed by reversed-phase liquid chromatography (RPLC). Proteins, phospholipids and highly polar interferences could be removed from plasma by the efficient combination of PPT, HILIC-SPE and RPLC-MS/MS. This method was evaluated by matrix effect, recovery and process efficiency at different concentration levels (50, 500 and 5,000 ng/mL) of triptorelin. Furthermore, the performance of HILIC-SPE was compared with that of reversed-phase C18 SPE and hydrophilic lipophilic balance (Oasis HLB) SPE. Among them, HILIC-SPE provided the minimum matrix effect (ranging from 96.02% to 103.41%), the maximum recovery (ranging from 80.68% to 90.54%) and the satisfactory process efficiency (ranging from 82.83% to 92.95%). The validated method was successfully applied to determine triptorelin in rat plasma. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24820974/Hydrophilic_interaction_liquid_chromatography_solid_phase_extraction_directly_combined_with_protein_precipitation_for_the_determination_of_triptorelin_in_plasma_ DB - PRIME DP - Unbound Medicine ER -