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Discriminating between the effects of founding events and reproductive mode on the genetic structure of Triops populations (Branchiopoda: Notostraca).
PLoS One. 2014; 9(5):e97473.Plos

Abstract

Crustaceans that initially colonize a freshwater temporary pond can strongly bias the subsequent genetic composition of the population, causing nearby populations to be genetically distinct. In addition, these crustaceans have various reproductive modes that can influence genetic differentiation and diversity within and between populations. We report on two species of tadpole shrimp, Triops newberryi and Triops longicaudatus "short", with different reproductive modes. Reproduction in the tadpole shrimp can occur clonally (parthenogenesis), with self fertilization (hermaphroditism), or through outcrossing of hermaphrodites with males (androdioecy). For all these reproductive modes, population genetic theory predicts decreased genetic diversity and increased population differentiation. Here we use mitochondrial control region (mtCR) sequences and nuclear microsatellite loci to determine if the difference in reproductive mode affects the high genetic structure typical of persistent founder effects. Previous authors indicated that T. newberryi is androdioecious because populations are composed of hermaphrodites and males, and T. longicaudatus "short" is hermaphroditic or parthenogenetic because males are absent. In our data, T. newberryi and T. longicaudatus "short" populations were highly structured genetically over short geographic distances for mtCR sequences and microsatellite loci (T. newberryi: ΦST = 0.644, FST = 0.252, respectively; T. l. "short": invariant mtCR sequences, FST = 0.600). Differences between the two Triops species in a number of diversity measures were generally consistent with expectations from population genetic theory regarding reproductive mode; however, three of four comparisons were not statistically significant. We conclude the high genetic differentiation between populations is likely due to founder effects and results suggest both species are composed of selfing hermaphrodites with some level of outcrossing; the presence of males in T. newberryi does not appreciably reduce inbreeding. We cannot exclude the possibility that males in T. newberryi are non-reproductive individuals and the two species have the same mating system.

Authors+Show Affiliations

Department of Fish, Wildlife & Conservation Ecology and Molecular Biology Program, New Mexico State University, Las Cruces, New Mexico, United States of America.Department of Fish, Wildlife & Conservation Ecology and Molecular Biology Program, New Mexico State University, Las Cruces, New Mexico, United States of America; Unit of Molecular Zoology, Chair of Zoology, Department of Animal Science, Technische Universität München, Freising, Germany.Unit of Molecular Zoology, Chair of Zoology, Department of Animal Science, Technische Universität München, Freising, Germany.Department of Fish, Wildlife & Conservation Ecology and Molecular Biology Program, New Mexico State University, Las Cruces, New Mexico, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24824220

Citation

Horn, Rebekah L., et al. "Discriminating Between the Effects of Founding Events and Reproductive Mode On the Genetic Structure of Triops Populations (Branchiopoda: Notostraca)." PloS One, vol. 9, no. 5, 2014, pp. e97473.
Horn RL, Kuehn R, Drechsel V, et al. Discriminating between the effects of founding events and reproductive mode on the genetic structure of Triops populations (Branchiopoda: Notostraca). PLoS ONE. 2014;9(5):e97473.
Horn, R. L., Kuehn, R., Drechsel, V., & Cowley, D. E. (2014). Discriminating between the effects of founding events and reproductive mode on the genetic structure of Triops populations (Branchiopoda: Notostraca). PloS One, 9(5), e97473. https://doi.org/10.1371/journal.pone.0097473
Horn RL, et al. Discriminating Between the Effects of Founding Events and Reproductive Mode On the Genetic Structure of Triops Populations (Branchiopoda: Notostraca). PLoS ONE. 2014;9(5):e97473. PubMed PMID: 24824220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discriminating between the effects of founding events and reproductive mode on the genetic structure of Triops populations (Branchiopoda: Notostraca). AU - Horn,Rebekah L, AU - Kuehn,Ralph, AU - Drechsel,Victoria, AU - Cowley,David E, Y1 - 2014/05/13/ PY - 2014/01/02/received PY - 2014/04/19/accepted PY - 2014/5/15/entrez PY - 2014/5/16/pubmed PY - 2015/6/2/medline SP - e97473 EP - e97473 JF - PloS one JO - PLoS ONE VL - 9 IS - 5 N2 - Crustaceans that initially colonize a freshwater temporary pond can strongly bias the subsequent genetic composition of the population, causing nearby populations to be genetically distinct. In addition, these crustaceans have various reproductive modes that can influence genetic differentiation and diversity within and between populations. We report on two species of tadpole shrimp, Triops newberryi and Triops longicaudatus "short", with different reproductive modes. Reproduction in the tadpole shrimp can occur clonally (parthenogenesis), with self fertilization (hermaphroditism), or through outcrossing of hermaphrodites with males (androdioecy). For all these reproductive modes, population genetic theory predicts decreased genetic diversity and increased population differentiation. Here we use mitochondrial control region (mtCR) sequences and nuclear microsatellite loci to determine if the difference in reproductive mode affects the high genetic structure typical of persistent founder effects. Previous authors indicated that T. newberryi is androdioecious because populations are composed of hermaphrodites and males, and T. longicaudatus "short" is hermaphroditic or parthenogenetic because males are absent. In our data, T. newberryi and T. longicaudatus "short" populations were highly structured genetically over short geographic distances for mtCR sequences and microsatellite loci (T. newberryi: ΦST = 0.644, FST = 0.252, respectively; T. l. "short": invariant mtCR sequences, FST = 0.600). Differences between the two Triops species in a number of diversity measures were generally consistent with expectations from population genetic theory regarding reproductive mode; however, three of four comparisons were not statistically significant. We conclude the high genetic differentiation between populations is likely due to founder effects and results suggest both species are composed of selfing hermaphrodites with some level of outcrossing; the presence of males in T. newberryi does not appreciably reduce inbreeding. We cannot exclude the possibility that males in T. newberryi are non-reproductive individuals and the two species have the same mating system. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24824220/Discriminating_between_the_effects_of_founding_events_and_reproductive_mode_on_the_genetic_structure_of_Triops_populations__Branchiopoda:_Notostraca__ L2 - http://dx.plos.org/10.1371/journal.pone.0097473 DB - PRIME DP - Unbound Medicine ER -