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Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis.
Fertil Steril. 2014 Jul; 102(1):151-159.e5.FS

Abstract

OBJECTIVE

To study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis.

DESIGN

Prospective cohort study.

SETTING

University ART center.

PATIENT(S)

Women with and without endometriosis undergoing ART (n=28).

INTERVENTION(S)

Follicular fluid (FF), granulosa cells (GCs), immature oocytes (IOs), and ART data on sibling cohort oocytes in groups A and B were compared.

MAIN OUTCOME MEASURE(S)

ART live birth outcomes, maturation, and aging among in vitro matured (IVM) oocytes, nitrate levels in FF, and nitrotyrosine (NT) footprints and apoptosis in the GCs.

RESULT(S)

Clinical characteristics and ART live birth outcomes were no different between groups A and B. Women from group A had significantly lower peak serum E2 (2,068.8±244.6 pg/mL vs. 2,756.2±205.0 pg/mL) and higher apoptosis (80.0% vs. 22.2%) and NT staining (70.0% vs. 22.2%) in GCs compared with group B. Fewer IOs underwent IVM to MII (0.6±0.3) in group A compared with group B (1.4±0.2). IVM oocytes had significantly higher incidence of cortical granule loss (83.3% vs. 24.0%) and spindle disruption (66.7% vs. 16.0%) and higher zona pellucida dissolution timing (133.8±9.4 s vs. 90.5±5.8 s) in group A compared with group B. FF nitrate levels were significantly higher in women who failed to conceive in group A (478.2±43.1 nmol/L) compared with those that did conceive (173.3±19.0 nmol/L).

CONCLUSION(S)

Increased protein nitration, GC apoptosis, resistance to IVM, and oocyte aging indicate the involvement of oxidative dysregulation of NO in the pathophysiology of altered follicular milieu and poor oocyte quality in women with endometriosis.

Authors+Show Affiliations

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan; Division of Reproductive Endocrinology and Infertility, University of California Davis Medical Center, Sacramento, California; Californa IVF Fertility Center, Davis and Sacramento, California. Electronic address: pravin.goud@ucdmc.ucdavis.edu.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan; California National Primate Research Center, University of California, Davis, California.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Georgia Regents University, Augusta, Georgia.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24825428

Citation

Goud, Pravin T., et al. "Dynamics of Nitric Oxide, Altered Follicular Microenvironment, and Oocyte Quality in Women With Endometriosis." Fertility and Sterility, vol. 102, no. 1, 2014, pp. 151-159.e5.
Goud PT, Goud AP, Joshi N, et al. Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis. Fertil Steril. 2014;102(1):151-159.e5.
Goud, P. T., Goud, A. P., Joshi, N., Puscheck, E., Diamond, M. P., & Abu-Soud, H. M. (2014). Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis. Fertility and Sterility, 102(1), 151-e5. https://doi.org/10.1016/j.fertnstert.2014.03.053
Goud PT, et al. Dynamics of Nitric Oxide, Altered Follicular Microenvironment, and Oocyte Quality in Women With Endometriosis. Fertil Steril. 2014;102(1):151-159.e5. PubMed PMID: 24825428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis. AU - Goud,Pravin T, AU - Goud,Anuradha P, AU - Joshi,Narendra, AU - Puscheck,Elizabeth, AU - Diamond,Michael P, AU - Abu-Soud,Husam M, Y1 - 2014/05/10/ PY - 2013/12/30/received PY - 2014/03/26/revised PY - 2014/03/27/accepted PY - 2014/5/15/entrez PY - 2014/5/16/pubmed PY - 2014/8/30/medline KW - Endometriosis KW - nitric oxide KW - oocyte aging KW - oocyte quality KW - peroxynitrite KW - superoxide SP - 151 EP - 159.e5 JF - Fertility and sterility JO - Fertil. Steril. VL - 102 IS - 1 N2 - OBJECTIVE: To study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis. DESIGN: Prospective cohort study. SETTING: University ART center. PATIENT(S): Women with and without endometriosis undergoing ART (n=28). INTERVENTION(S): Follicular fluid (FF), granulosa cells (GCs), immature oocytes (IOs), and ART data on sibling cohort oocytes in groups A and B were compared. MAIN OUTCOME MEASURE(S): ART live birth outcomes, maturation, and aging among in vitro matured (IVM) oocytes, nitrate levels in FF, and nitrotyrosine (NT) footprints and apoptosis in the GCs. RESULT(S): Clinical characteristics and ART live birth outcomes were no different between groups A and B. Women from group A had significantly lower peak serum E2 (2,068.8±244.6 pg/mL vs. 2,756.2±205.0 pg/mL) and higher apoptosis (80.0% vs. 22.2%) and NT staining (70.0% vs. 22.2%) in GCs compared with group B. Fewer IOs underwent IVM to MII (0.6±0.3) in group A compared with group B (1.4±0.2). IVM oocytes had significantly higher incidence of cortical granule loss (83.3% vs. 24.0%) and spindle disruption (66.7% vs. 16.0%) and higher zona pellucida dissolution timing (133.8±9.4 s vs. 90.5±5.8 s) in group A compared with group B. FF nitrate levels were significantly higher in women who failed to conceive in group A (478.2±43.1 nmol/L) compared with those that did conceive (173.3±19.0 nmol/L). CONCLUSION(S): Increased protein nitration, GC apoptosis, resistance to IVM, and oocyte aging indicate the involvement of oxidative dysregulation of NO in the pathophysiology of altered follicular milieu and poor oocyte quality in women with endometriosis. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/24825428/Dynamics_of_nitric_oxide_altered_follicular_microenvironment_and_oocyte_quality_in_women_with_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(14)00308-2 DB - PRIME DP - Unbound Medicine ER -