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Characterization of enterohemorrhagic Escherichia coli O111 and O157 strains isolated from outbreak patients in Japan.
J Clin Microbiol. 2014 Aug; 52(8):2757-63.JC

Abstract

In April and May 2011, there was a serious food-poisoning outbreak in Japan caused by enterohemorrhagic Escherichia coli (EHEC) strains O111:H8 and O157:H7 from raw beef dishes at branches of a barbecue restaurant. This outbreak involved 181 infected patients, including 34 hemolytic-uremic syndrome (HUS) cases (19%). Among the 34 HUS patients, 21 developed acute encephalopathy (AE) and 5 died. Patient stool specimens yielded E. coli O111 and O157 strains. We also detected both EHEC O111 stx2 and stx-negative E. coli O111 strains in a stock of meat block from the restaurant. Pulsed-field gel electrophoresis (PFGE) and multilocus variable-number tandem-repeat analysis (MLVA) showed that the stx-negative E. coli O111 isolates were closely related to EHEC O111 stx2 isolates. Although the EHEC O157 strains had diverse stx gene profiles (stx1, stx2, and stx1 stx2), the PFGE and MLVA analyses indicated that these isolates originated from a single clone. Deletion of the Stx2-converting prophage from the EHEC O111 stx2 isolates was frequently observed during in vitro growth, suggesting that strain conversion from an EHEC O111 stx2 to an stx-negative strain may have occurred during infection.

Authors+Show Affiliations

Toyama Institute of Health, Toyama, Japan masanori.watahiki@pref.toyama.lg.jp.Toyama Institute of Health, Toyama, Japan.Toyama Institute of Health, Toyama, Japan.Toyama Institute of Health, Toyama, Japan.Toyama Institute of Health, Toyama, Japan.Toyama Institute of Health, Toyama, Japan.Fukui Prefectural Institute of Public Health and Environment Science, Fukui, Japan.Ishikawa Prefectural Institute of Public Health and Environmental Science, Ishikawa, Japan.Yokohama City Institute of Health, Kanagawa, Japan.National Institute of Infectious Diseases, Tokyo, Japan.National Institute of Infectious Diseases, Tokyo, Japan.National Institute of Infectious Diseases, Tokyo, Japan.National Institute of Infectious Diseases, Tokyo, Japan.National Institute of Infectious Diseases, Tokyo, Japan.National Institute of Infectious Diseases, Tokyo, Japan.Toyama Institute of Health, Toyama, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24829231

Citation

Watahiki, Masanori, et al. "Characterization of Enterohemorrhagic Escherichia Coli O111 and O157 Strains Isolated From Outbreak Patients in Japan." Journal of Clinical Microbiology, vol. 52, no. 8, 2014, pp. 2757-63.
Watahiki M, Isobe J, Kimata K, et al. Characterization of enterohemorrhagic Escherichia coli O111 and O157 strains isolated from outbreak patients in Japan. J Clin Microbiol. 2014;52(8):2757-63.
Watahiki, M., Isobe, J., Kimata, K., Shima, T., Kanatani, J., Shimizu, M., Nagata, A., Kawakami, K., Yamada, M., Izumiya, H., Iyoda, S., Morita-Ishihara, T., Mitobe, J., Terajima, J., Ohnishi, M., & Sata, T. (2014). Characterization of enterohemorrhagic Escherichia coli O111 and O157 strains isolated from outbreak patients in Japan. Journal of Clinical Microbiology, 52(8), 2757-63. https://doi.org/10.1128/JCM.00420-14
Watahiki M, et al. Characterization of Enterohemorrhagic Escherichia Coli O111 and O157 Strains Isolated From Outbreak Patients in Japan. J Clin Microbiol. 2014;52(8):2757-63. PubMed PMID: 24829231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of enterohemorrhagic Escherichia coli O111 and O157 strains isolated from outbreak patients in Japan. AU - Watahiki,Masanori, AU - Isobe,Junko, AU - Kimata,Keiko, AU - Shima,Tomoko, AU - Kanatani,Jun-ichi, AU - Shimizu,Miwako, AU - Nagata,Akihiro, AU - Kawakami,Keiko, AU - Yamada,Mikiko, AU - Izumiya,Hidemasa, AU - Iyoda,Sunao, AU - Morita-Ishihara,Tomoko, AU - Mitobe,Jiro, AU - Terajima,Jun, AU - Ohnishi,Makoto, AU - Sata,Tetsutaro, Y1 - 2014/05/14/ PY - 2014/5/16/entrez PY - 2014/5/16/pubmed PY - 2015/5/21/medline SP - 2757 EP - 63 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 52 IS - 8 N2 - In April and May 2011, there was a serious food-poisoning outbreak in Japan caused by enterohemorrhagic Escherichia coli (EHEC) strains O111:H8 and O157:H7 from raw beef dishes at branches of a barbecue restaurant. This outbreak involved 181 infected patients, including 34 hemolytic-uremic syndrome (HUS) cases (19%). Among the 34 HUS patients, 21 developed acute encephalopathy (AE) and 5 died. Patient stool specimens yielded E. coli O111 and O157 strains. We also detected both EHEC O111 stx2 and stx-negative E. coli O111 strains in a stock of meat block from the restaurant. Pulsed-field gel electrophoresis (PFGE) and multilocus variable-number tandem-repeat analysis (MLVA) showed that the stx-negative E. coli O111 isolates were closely related to EHEC O111 stx2 isolates. Although the EHEC O157 strains had diverse stx gene profiles (stx1, stx2, and stx1 stx2), the PFGE and MLVA analyses indicated that these isolates originated from a single clone. Deletion of the Stx2-converting prophage from the EHEC O111 stx2 isolates was frequently observed during in vitro growth, suggesting that strain conversion from an EHEC O111 stx2 to an stx-negative strain may have occurred during infection. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/24829231/Characterization_of_enterohemorrhagic_Escherichia_coli_O111_and_O157_strains_isolated_from_outbreak_patients_in_Japan_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=24829231 DB - PRIME DP - Unbound Medicine ER -