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Determination of toxins involved in ciguatera fish poisoning in the Pacific by LC/MS.
J AOAC Int. 2014 Mar-Apr; 97(2):398-402.JA

Abstract

Ciguatera fish poisoning is the most extensive and difficult to control of the seafood poisonings. To facilitate monitoring of fish toxicity, toxin profiles were investigated by an LC/MS/MS method using 14 reference toxins on eight representative species of fish collected in four different areas of the Pacific. Snappers and groupers from Okinawa contained ciguatoxin-1B (CTX1B) and two deoxy congeners at variable but species-specific ratios, while red snapper, Lutjanus bohar, from Minamitorishima, and amberjack, Seriola dumerili, from Hawaii, contained both CTX1B-type and CTX3C-type toxins. Spotted knifejaw, Oplegnathus punctatus, from Okinawan waters, contained mainly CTX4A and CTX4B, but the same species caught at Miyazaki was contaminated primarily with the CTX3C-type toxins. Otherwise, the toxin profiles were consistently species-specific in fish collected from various locations around Okinawa over 20 years. The LC/MS/MS and mouse bioassay results agreed well, indicating the LC/MS/MS method is a promising alternative to the mouse bioassay. Pure CTX1B and CTX3C were prepared for use in future LC/MS/MS analysis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24830151

Citation

Yogi, Kentaro, et al. "Determination of Toxins Involved in Ciguatera Fish Poisoning in the Pacific By LC/MS." Journal of AOAC International, vol. 97, no. 2, 2014, pp. 398-402.
Yogi K, Sakugawa S, Oshiro N, et al. Determination of toxins involved in ciguatera fish poisoning in the Pacific by LC/MS. J AOAC Int. 2014;97(2):398-402.
Yogi, K., Sakugawa, S., Oshiro, N., Ikehara, T., Sugiyama, K., & Yasumoto, T. (2014). Determination of toxins involved in ciguatera fish poisoning in the Pacific by LC/MS. Journal of AOAC International, 97(2), 398-402.
Yogi K, et al. Determination of Toxins Involved in Ciguatera Fish Poisoning in the Pacific By LC/MS. J AOAC Int. 2014 Mar-Apr;97(2):398-402. PubMed PMID: 24830151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of toxins involved in ciguatera fish poisoning in the Pacific by LC/MS. AU - Yogi,Kentaro, AU - Sakugawa,Satsuki, AU - Oshiro,Naomasa, AU - Ikehara,Tsuyoshi, AU - Sugiyama,Kiminori, AU - Yasumoto,Takeshi, PY - 2014/5/17/entrez PY - 2014/5/17/pubmed PY - 2014/6/3/medline SP - 398 EP - 402 JF - Journal of AOAC International JO - J AOAC Int VL - 97 IS - 2 N2 - Ciguatera fish poisoning is the most extensive and difficult to control of the seafood poisonings. To facilitate monitoring of fish toxicity, toxin profiles were investigated by an LC/MS/MS method using 14 reference toxins on eight representative species of fish collected in four different areas of the Pacific. Snappers and groupers from Okinawa contained ciguatoxin-1B (CTX1B) and two deoxy congeners at variable but species-specific ratios, while red snapper, Lutjanus bohar, from Minamitorishima, and amberjack, Seriola dumerili, from Hawaii, contained both CTX1B-type and CTX3C-type toxins. Spotted knifejaw, Oplegnathus punctatus, from Okinawan waters, contained mainly CTX4A and CTX4B, but the same species caught at Miyazaki was contaminated primarily with the CTX3C-type toxins. Otherwise, the toxin profiles were consistently species-specific in fish collected from various locations around Okinawa over 20 years. The LC/MS/MS and mouse bioassay results agreed well, indicating the LC/MS/MS method is a promising alternative to the mouse bioassay. Pure CTX1B and CTX3C were prepared for use in future LC/MS/MS analysis. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/24830151/Determination_of_toxins_involved_in_ciguatera_fish_poisoning_in_the_Pacific_by_LC/MS_ L2 - https://academic.oup.com/jaoac/article-lookup/doi/10.5740/jaoacint.sgeyogi DB - PRIME DP - Unbound Medicine ER -