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Antibodies in the diagnosis of coeliac disease: a biopsy-controlled, international, multicentre study of 376 children with coeliac disease and 695 controls.
PLoS One. 2014; 9(5):e97853.Plos

Abstract

Diagnosis of coeliac disease (CD) relies on a combination of clinical, genetic, serological and duodenal morphological findings. The ESPGHAN suggested that biopsy may not be necessary in all cases. New guidelines include omission of biopsy if the concentration of CD-specific antibodies exceeds 10 times the upper limit of normal (10 ULN) and other criteria are met. We analysed the 10 ULN criterion and investigated multiple antibody-assays. Serum was collected from 1071 children with duodenal biopsy (376 CD patients, 695 disease-controls). IgA-antibodies to tissue transglutaminase (IgA-aTTG), IgG-antibodies to deamidated gliadin peptides (IgG-aDGL) and IgA-endomysium antibodies (IgA-EMA) were measured centrally. We considered 3 outcomes for antibody test procedures utilizing IgA-aTTG and/or IgG-aDGL: positive (≥10 ULN, recommend gluten-free diet), negative (<1 ULN, no gluten-free diet) or unclear (perform biopsy). Positive (PPV) and negative (NPV) predictive values were based on clear test results. We required that they and their lower confidence bounds (LCB) be simultaneously very high (LCB >90% and PPV/NPV >95%). These stringent conditions were met for appropriate antibody-procedures over a prevalence range of 9-57%. By combining IgG-aDGL with IgA-aTTG, one could do without assaying total IgA. The PPV of IgG-aDGL was estimated to be extremely high, although more studies are necessary to narrow down the LCB. The proportion of patients requiring a biopsy was <11%. The procedures were either equivalent or even better in children <2 years compared to older children. All 310 of the IgA-aTTG positive children were also IgA-EMA positive. Antibody-assays could render biopsies unnecessary in most children, if experienced paediatric gastroenterologists evaluate the case. This suggestion only applies to the kits used here and should be verified for other available assays. Confirming IgA-aTTG positivity (≥10 ULN) by EMA-testing is unnecessary if performed on the same blood sample. Prospective studies are needed.

Authors+Show Affiliations

Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, Medical Faculty of the University and University Hospital, Leipzig, Germany.Institute for Medical Informatics, Statistics & Epidemiology of the University, Leipzig, Leipzig, Germany.Coordination Centre for Clinical Trials of the University, Leipzig, Germany.Children's Hospital of the Clinical Centre "Sankt Georg", Leipzig, Germany.Translational Gastroenterology Unit, Experimental Medicine, University of Oxford, John Radcliffe Hospital, Oxford, England.No affiliation info availableUniversity Children's Hospital, Graz, Austria.University Children's Hospital, Tübingen, Germany.Laboratory Medicine, Immunology, University Hospitals Leuven, Catholic University, Leuven, Belgium.University Children's Hospital, Gieβen, Germany.University Children's Hospital, Leipzig, Germany.Allergy and Clinical Immunology Unit, Azienda Ospedaliera "San Maria degli Angeli", Pordenone, Italy.EUROIMMUN AG Labormedizinische Diagnostika AG, Lübeck, Germany.Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, Medical Faculty of the University and University Hospital, Leipzig, Germany.

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24830313

Citation

Wolf, Johannes, et al. "Antibodies in the Diagnosis of Coeliac Disease: a Biopsy-controlled, International, Multicentre Study of 376 Children With Coeliac Disease and 695 Controls." PloS One, vol. 9, no. 5, 2014, pp. e97853.
Wolf J, Hasenclever D, Petroff D, et al. Antibodies in the diagnosis of coeliac disease: a biopsy-controlled, international, multicentre study of 376 children with coeliac disease and 695 controls. PLoS One. 2014;9(5):e97853.
Wolf, J., Hasenclever, D., Petroff, D., Richter, T., Uhlig, H. H., Laaβ, M. W., Hauer, A., Stern, M., Bossuyt, X., de Laffolie, J., Flemming, G., Villalta, D., Schlumberger, W., & Mothes, T. (2014). Antibodies in the diagnosis of coeliac disease: a biopsy-controlled, international, multicentre study of 376 children with coeliac disease and 695 controls. PloS One, 9(5), e97853. https://doi.org/10.1371/journal.pone.0097853
Wolf J, et al. Antibodies in the Diagnosis of Coeliac Disease: a Biopsy-controlled, International, Multicentre Study of 376 Children With Coeliac Disease and 695 Controls. PLoS One. 2014;9(5):e97853. PubMed PMID: 24830313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibodies in the diagnosis of coeliac disease: a biopsy-controlled, international, multicentre study of 376 children with coeliac disease and 695 controls. AU - Wolf,Johannes, AU - Hasenclever,Dirk, AU - Petroff,David, AU - Richter,Thomas, AU - Uhlig,Holm H, AU - Laaβ,Martin W, AU - Hauer,Almuthe, AU - Stern,Martin, AU - Bossuyt,Xavier, AU - de Laffolie,Jan, AU - Flemming,Gunter, AU - Villalta,Danilo, AU - Schlumberger,Wolfgang, AU - Mothes,Thomas, Y1 - 2014/05/15/ PY - 2014/02/20/received PY - 2014/04/25/accepted PY - 2014/5/17/entrez PY - 2014/5/17/pubmed PY - 2015/6/16/medline SP - e97853 EP - e97853 JF - PloS one JO - PLoS One VL - 9 IS - 5 N2 - Diagnosis of coeliac disease (CD) relies on a combination of clinical, genetic, serological and duodenal morphological findings. The ESPGHAN suggested that biopsy may not be necessary in all cases. New guidelines include omission of biopsy if the concentration of CD-specific antibodies exceeds 10 times the upper limit of normal (10 ULN) and other criteria are met. We analysed the 10 ULN criterion and investigated multiple antibody-assays. Serum was collected from 1071 children with duodenal biopsy (376 CD patients, 695 disease-controls). IgA-antibodies to tissue transglutaminase (IgA-aTTG), IgG-antibodies to deamidated gliadin peptides (IgG-aDGL) and IgA-endomysium antibodies (IgA-EMA) were measured centrally. We considered 3 outcomes for antibody test procedures utilizing IgA-aTTG and/or IgG-aDGL: positive (≥10 ULN, recommend gluten-free diet), negative (<1 ULN, no gluten-free diet) or unclear (perform biopsy). Positive (PPV) and negative (NPV) predictive values were based on clear test results. We required that they and their lower confidence bounds (LCB) be simultaneously very high (LCB >90% and PPV/NPV >95%). These stringent conditions were met for appropriate antibody-procedures over a prevalence range of 9-57%. By combining IgG-aDGL with IgA-aTTG, one could do without assaying total IgA. The PPV of IgG-aDGL was estimated to be extremely high, although more studies are necessary to narrow down the LCB. The proportion of patients requiring a biopsy was <11%. The procedures were either equivalent or even better in children <2 years compared to older children. All 310 of the IgA-aTTG positive children were also IgA-EMA positive. Antibody-assays could render biopsies unnecessary in most children, if experienced paediatric gastroenterologists evaluate the case. This suggestion only applies to the kits used here and should be verified for other available assays. Confirming IgA-aTTG positivity (≥10 ULN) by EMA-testing is unnecessary if performed on the same blood sample. Prospective studies are needed. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24830313/Antibodies_in_the_diagnosis_of_coeliac_disease:_a_biopsy_controlled_international_multicentre_study_of_376_children_with_coeliac_disease_and_695_controls_ L2 - https://dx.plos.org/10.1371/journal.pone.0097853 DB - PRIME DP - Unbound Medicine ER -